DNA Analysis by Application of Pt Nanoparticle Electrochemical Amplification with Single Label Response

Kwon, Seong Jung; Bard, Allen J.

doi:10.1021/ja304074f

Cited by 181 publications

(118 citation statements)

References 28 publications

(46 reference statements)

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“…Because of the success in studying many systems using collisions, such as early work using electrocatalytic amplification as a sensitive means of detecting DNA (23), it was our hope to extend the field of collisions to more biologically relevant species, such as viruses. Cytomegaloviruses (CMVs) are the prototypic members of the β-herpesvirus family.…”

mentioning

confidence: 99%

Electrochemical detection of a single cytomegalovirus at an ultramicroelectrode and its antibody anchoring

Dick

Hilterbrand

Boika

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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143

132

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We report observations of stochastic collisions of murine cytomegalovirus (MCMV) on ultramicroelectrodes (UMEs), extending the observation of discrete collision events on UMEs to biologically relevant analytes. Adsorption of an antibody specific for a virion surface glycoprotein allowed differentiation of MCMV from MCMV bound by antibody from the collision frequency decrease and current magnitudes in the electrochemical collision experiments, which shows the efficacy of the method to size viral samples. To add selectivity to the technique, interactions between MCMV, a glycoprotein-specific primary antibody to MCMV, and polystyrene bead "anchors," which were functionalized with a secondary antibody specific to the Fc region of the primary antibody, were used to affect virus mobility. Bead aggregation was observed, and the extent of aggregation was measured using the electrochemical collision technique. Scanning electron microscopy and optical microscopy further supported aggregate shape and extent of aggregation with and without MCMV. This work extends the field of collisions to biologically relevant antigens and provides a novel foundation upon which qualitative sensor technology might be built for selective detection of viruses and other biologically relevant analytes.collisions | cytomegalovirus | electrochemistry | murine cytomegalovirus | virus O ver the past decade, the study of discrete collision events on ultramicroelectrodes (UMEs) has gained attention due to the interest in understanding stochastic phenomena by electrochemistry. By observing the collisions of small particles, there is the possibility that information can be deduced that is not available in ensemble measurements. The electrochemical study of single collision events has been applied to a wide range of hard nanoparticles (NPs), which include metal, metal oxide, and organic NPs [platinum (1), silver (2), gold (3), nickel (4), copper (5), iridium oxide (6), cerium oxide (7), titanium oxide (8), silicon oxide (9), indigo (10), polystyrene (11), and relatively large aggregates of fullerene (12)]. Recently, collisions of soft particles have been investigated, such as toluene droplets (13) and liposomes (14). Also, collisions of toluene and tri-n-propylamine droplets were observed simultaneously by both electrochemical and electrogenerated chemiluminescent (ECL) measurements (15).Similarly, a variety of techniques have been developed to observe these collision events. The interested reader can consult the references for a discussion on each of the techniques used to observe stochastic events electrochemically: blocking (9, 13), electrocatalytic amplification (1), open circuit potential (16), droplet blocking/reactor (13,14), and ECL (15,17,18). The simplest and most reproducible method of observing collisions is a technique termed blocking, which is so named because particles, which are brought to the electrode by a diffusion-limited flux and/or electrophoretic migration, irreversibly adsorb (1) to the electrode surface, blocking the flux of red...

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confidence: 99%

Electrochemical detection of a single cytomegalovirus at an ultramicroelectrode and its antibody anchoring

Dick

Hilterbrand

Boika

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

143

132

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“…In optic-based biosensors, single molecule detection, such as DNA, can be done [27]. This technology was later improved due to innovations like combination of biological materials.…”

Section: Technological Comparisonmentioning

confidence: 99%

Application of Electrochemical Methods in Biosensing Technologies

Dziąbowska¹,

Czaczyk²,

Nidzworski³

2018

Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis

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Introducing biochemical factor to electronic devices have created a new branch of science. Recent development in biosensing technology enabled progress in pathogens detection. Currently, wide range of biomarkers (enzymes, peptides, DNA, microorganisms, etc. ) recognize various target analytes, starting from basic metabolism changes to serious infections caused by pathogens. Improved sensitivity, selectivity and response time of sensors have instantly replaced traditional techniques. Easy handling, low production costs and miniaturization have met therapeutics need. Biosensing technologies are very strong point in telemedicine in public healthcare. This chapter will focus on electrochemical techniques for pathogens detection and show trending applications in biosensing technologies.

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“…In another study, Tang et al demonstrated antigen-antibody interaction via GOD conjugated hollow gold-silver microspheres (AuAgHSs) further coupled using Prussian blue NPs (an artificial catalase), on a graphene-based immunosensor [117]. Pesticidal study [96] Hba1c biosensor Determining glycated hemoglobin [97] Piezoelectric biosensors Detecting carbamate and organophosphate [98] Uric acid biosensors Diagnosis of various clinical abnormalities or illness (e.g., diagnosis of cardiovascular disease) [99,100] Glucose oxidase electrode biosensors Measuring of glucose in biological sample of diabetic patient [101] Quartz-crystal biosensors Detection of proteins at ultrahigh-sensitive level in liquids [102] Optical Polyacrylamide based hydrogel biosensors Immobilization of biomolecules [30] Silicon biosensor For biosensing, bioimaging, and in cancer therapy [103,104] Microfabricated biosensor In novel drug delivery (e.g., in optical corrections) [105] Fluorescence tagged/Genetically encoded biosensor Investigation of various biological process and molecular systems in the cell [27,[106][107][108] Electrochemical or optical Nanomaterials biosensors For diagnosis and in drug delivery [32,93,[109][110][111][112][113][114] …”

Section: Glucose Monitoringmentioning

confidence: 99%

Recent Advances and Applications of Biosensors in Novel Technology

Rajpoot¹

2017

Biosens J

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In recent time, several novel biosensors such as enzyme based, immunosensors, tissue-based, DNA biosensors, piezoelectric, and thermal biosensors have been explored with high sensitivity by many research groups. These biosensors exhibit many essential benefits, including operational simplicity, remarkable sensitivity, low-cost instrumentation, the potential of automation, inherent miniaturization. They have offered elegant paths for the detection of even trace amounts of analytical agents of biological significance existing from macromolecules (e.g., disease biomarkers and antigens) and small molecules (e.g., natural toxins and haptens) to cells, viruses, and bacteria. Many electrochemical techniques viz., amperometry and voltammetry, photoelectrochemistry, electrochemiluminescence, impedance, piezoelectricity, potentiometry, alternating current electrohydrodynamics, and field-effect transistor have been utilized in the development of immunoassays to attain high sensitivity for electrochemical signal transduction. Recent developments in biological methods and instrumentation after using fluorescence tag to various nanocarriers such as nanoparticles, nanowires, nanotubes, etc. have improved the sensitivity of biosensors. Utilization of nucleotides/aptamers, affibodies, molecule imprinted polymers, and peptide arrays offer great tools to prepare advanced biosensors. Merging of nanotechnology with biosensor systems enhanced the diagnostic capability. This review gives an outline of the advances in biosensors technology relating biomedical sciences.

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DNA Analysis by Application of Pt Nanoparticle Electrochemical Amplification with Single Label Response

Cited by 181 publications

References 28 publications

Electrochemical detection of a single cytomegalovirus at an ultramicroelectrode and its antibody anchoring

Electrochemical detection of a single cytomegalovirus at an ultramicroelectrode and its antibody anchoring

Application of Electrochemical Methods in Biosensing Technologies

Recent Advances and Applications of Biosensors in Novel Technology

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