DNA analyses and their applications in plant breeding

Ovesná, Jaroslava; Poláková, Kateřina Machová; Leišová, L.

doi:10.17221/6108-cjgpb

Cited by 49 publications

(21 citation statements)

References 105 publications

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“…This coincides with the observations found in other monocots, viz., rice bean and barley by Muthusamy and Fernandez et al,respectively [42,43]. AFLP technology is another powerful tool for detection and evolution in germplasm collections and in the screening of biodiversity as well as for fingerprinting studies [44][45][46]. It is also clear from the present investigation that AFLP technique was able to differentiate intraspecific relations of the Curcuma species by a higher number of unique markers compared to RAPD and ISSR techniques.…”

Section: Discussionmentioning

confidence: 50%

Genetic Relationship of Curcuma Species from Northeast India Using PCR-Based Markers

et al. 2011

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Molecular genetic fingerprints of nine Curcuma species from Northeast India were developed using PCR-based markers. The aim involves elucidating there intra- and inter-specific genetic diversity important for utilization, management, and conservation. Twelve random amplified polymorphic DNA (RAPD), 19 Inter simple sequence repeats (ISSRs), and four amplified fragment length polymorphism (AFLP) primers produced 266 polymorphic fragments. ISSR confirmed maximum polymorphism of 98.55% whereas RAPD and AFLP showed 93.22 and 97.27%, respectively. Marker index and polymorphic information content varied in the range of 8.64-48.1, 19.75-48.14, and 25-28 and 0.17-0.48, 0.19-0.48, and 0.25-0.29 for RAPD, ISSR, and AFLP markers, respectively. The average value of number of observed alleles, number of effective alleles, mean Nei's gene diversity, and Shannon's information index were 1.93-1.98, 1.37-1.62, 0.23-0.36, and 0.38-0.50, respectively, for three DNA markers used. Dendrograms based on three molecular data using unweighted pair group method with arithmetic mean (UPGMA) was congruent and classified the Curcuma species into two major clusters. Cophenetic correlation coefficient between dendrogram and original similarity matrix were significant for RAPD (r = 0.96), ISSR (r = 0.94), and AFLP (r = 0.97). Clustering was further supported by principle coordinate analysis. High genetic polymorphism documented is significant for conservation and further improvement of Curcuma species.

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Section: Discussionmentioning

confidence: 50%

Genetic Relationship of Curcuma Species from Northeast India Using PCR-Based Markers

et al. 2011

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“…Among others, it is due to different sensitivity, reproducibility and reliability of the methods employed. The AFLP method is known to be highly sensitive and more reliable as compared to RAPD method (Ovesná et al 2002).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of spring barley genotypes with different susceptibility to Fusarium head blight using molecular markers

Nesvadba¹,

Vyhnánek²,

Ježíšková³

et al. 2006

PLANT SOIL ENVIRON

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485Fusarium head blight (FHB) is a worldwide distributed cereal disease that is caused by fungi of the Fusarium genus. Infection of plants can result in yield reduction and accumulation of toxic secondary fungal metabolites, mycotoxins in kernels (Windels 2000, Takeda 2004). The number of papers dealing with FHB on barley is generally much lower than that of research studies on wheat. It is due to a much greater economic importance of wheat as a cereal crop, but as well as to the fact that FHB causes larger economic losses in wheat than in barley. Though the two crops are attacked by identical FHB pathogens, there are differences in their infection responses. In general, barley is less susceptible to FHB than wheat. Grain yield is affected by FHB more in wheat than in barley, however a higher mycotoxin concentration is observed in the latter due to outer cover layers (lemma and palea) that are parts of the kernel from harvest to end use (Steffenson 2003). Produced toxins are harmful to human health and can induce serious diseases. The mycotoxins are deactivated neither in malting nor in brewing processes. Moreover, they can influence beer flavour and cause gushEvaluation of spring barley genotypes with different susceptibility to Fusarium head blight using molecular markers ABSTRACTOne of cereal diseases that has gained a greater importance for growers, processing industry as well as for breeders in many regions of the world is Fusarium head blight (FHB) caused by fungi of the Fusarium genus. The objectives of this study were to test diversity among spring barley breeding lines exhibiting various sensitivity to FHB and to find RAPD markers and AFLP markers that will distinguish between susceptible and resistant and/or moderately resistant genotypes. A test of a set of spring barley genotypes artificially infected by fusaria in field trials was carried out. Based on the results from field and laboratory evaluation and deoxynivalenol (DON) content assessment, barley genotypes with different responses to FHB were selected. The genotypes were hybridized and doubled haploid (DH) lines were derived in F 1 generation using the in vitro androgenesis method. Initial parental components and derived DH lines were tested for FHB infection and DON content. A set of parental genotypes of spring barley was tested with 80 RAPD markers. A RAPD marker (H30) was detected which enabled to distinguish between very susceptible parental genotypes and other resistant or moderately resistant spring barley genotypes based on the fragment of about 1300 bp. This specific product was screened in 23 DH lines derived from crosses of parental genotypes of spring barley and detected in 10 DH lines. During the study, some DH lines were selected that exhibited improved resistance to Fusarium infection. A low infection level and low DON content was found in the line DH 4/2 derived from CI 4196 × Foster. The AFLP technique was used to analyse parental genotypes of spring barley. The detected markers can be further evaluated and employed to select ...

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“…Specific DNA profiles were generated for each line, which can make further evaluation of marker segregation possible. Especially SSR profiles of individual lines can be further used to monitor the share of parental genomes by backcrossing to select most promising lines (Ovesná et al 2002). Based on preliminary screening susceptible line PI 383933, resistant line PEC 210 and resistant cultivar Chevron were used for DH lines development.…”

Section: Resultsmentioning

confidence: 99%

The use of molecular markers for characterisation of spring barley for breeding to Fusarium head blight resistance

Špunarová¹,

Ovesná²,

Tvarůžek³

et al. 2005

PLANT SOIL ENVIRON

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Fusarium head blight (FHB) is a barley disease, which occurs every year in various areas of barley cultivation all over the world and the increasing incidence has been confirmed in the Czech Republic also during the last years. We aimed to emply AFLP (Amplified fragment length polymorphism) and SSR (Single sequence repeats) markers to describe diversity among breeding lines with a sufficient level of resistance towards FHB and to find marker(s) associated with the analysed traits. The number of eight accessions including five expected resistance donors and three sensitive lines were tested in the field and laboratory. The field values and the amount of deoxynivalenol were positively correlated (r = 0.92). The laboratory test and content DON manifested also a high correlation (r = 0.73). Several DH lines developed from androgenetic barley progenies of the F1 hybrids between the susceptible line PI 383933 and resistant line PEC 210 or the susceptible line PI 383933 and resistant cultivar Chevron were found resistant towards Fusarium infection in both the field and laboratory tests. Low infestation was found at line DH 37 from combination Chevron × PI 383933 and lines DH48, DH49, DH50 and DH55 from the combination PEC 210 × PI 383933. Cluster analyses based on 68 AFLP and 18 SSR markers demonstrate a genetic relationship among parental genotypes and DH lines. Some DH lines combined a sufficient degree of resistance against FHB and extract content (basic parameters malting quality). Statistically significant differences in malt-extract values were observed between groups of the DH lines possessing and not possessing the AFLP marker CAA/AGC 341bp. The markers will be further evaluated and optionally used for MAS.

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DNA analyses and their applications in plant breeding

Cited by 49 publications

References 105 publications

Genetic Relationship of Curcuma Species from Northeast India Using PCR-Based Markers

Genetic Relationship of Curcuma Species from Northeast India Using PCR-Based Markers

Evaluation of spring barley genotypes with different susceptibility to Fusarium head blight using molecular markers

The use of molecular markers for characterisation of spring barley for breeding to Fusarium head blight resistance

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