DNA Alignment-Induced Linear Dichroism via Magnetic Field Assisted Electrospray: Implications for Optical Applications

Choi, Eunsik; Lee, Sang-Ho; Kang, Kyung‐Tae

doi:10.1021/acsanm.3c00163

Cited by 2 publications

(1 citation statement)

References 30 publications

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“…Applying a weaker electric field promotes the electrophoretic movement of DNA in electrophoretic gels, which can be used to align long DNA molecules due to the mechanical interaction between the DNA and the gel fibers [27]. Alignment of single-stranded DNA has been reported using a very strong magnetic field [28].…”

Section: Application Of Electric or Magnetic Fieldmentioning

confidence: 99%

Linear Dichroism Measurements for the Study of Protein-DNA Interactions

Takahashi,

Norden

2023

IJMS

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Linear dichroism (LD) is a differential polarized light absorption spectroscopy used for studying filamentous molecules such as DNA and protein filaments. In this study, we review the applications of LD for the analysis of DNA-protein interactions. LD signals can be measured in a solution by aligning the sample using flow-induced shear force or a strong electric field. The signal generated is related to the local orientation of chromophores, such as DNA bases, relative to the filament axis. LD can thus assess the tilt and roll of DNA bases and distinguish intercalating from groove-binding ligands. The intensity of the LD signal depends upon the degree of macroscopic orientation. Therefore, DNA shortening and bending can be detected by a decrease in LD signal intensity. As examples of LD applications, we present a kinetic study of DNA digestion by restriction enzymes and structural analyses of homologous recombination intermediates, i.e., RecA and Rad51 recombinase complexes with single-stranded DNA. LD shows that the DNA bases in these complexes are preferentially oriented perpendicular to the filament axis only in the presence of activators, suggesting the importance of organized base orientation for the reaction. LD measurements detect DNA bending by the CRP transcription activator protein, as well as by the UvrB DNA repair protein. LD can thus provide information about the structures of protein-DNA complexes under various conditions and in real time.

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Section: Application Of Electric or Magnetic Fieldmentioning

confidence: 99%

Linear Dichroism Measurements for the Study of Protein-DNA Interactions

Takahashi,

Norden

2023

IJMS

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Liquid Metal Electrodynamic Accumulation Microfluidics System for DNA Memory and Liquid Biopsy

Jeong,

Noh,

et al. 2023

Adv Funct Materials

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This study presents a liquid metal electrodynamic accumulation microfluidic (LEAM) device that effectively controls DNA dynamics using liquid metal (LM) electrodes and an applied electric field. The characteristics of LM in LEAM include electrochemical attack, and LM properties and behavior at high voltages. The findings demonstrate the ability of the LEAM system to control DNA dynamics and thermodynamics. Short‐sequence single stranded DNA (ssDNA) hybridization and displacement are performed using an exchange buffer solution. By scaling up the LEAM system for a droplet‐based approach, discrete manipulation of DNA samples is achieved. A three‐bit molecular ssDNA memory system using a molecular beacon system is demonstrated, and its application is extended as a diagnostic tool for detecting gene molecules such as micro‐RNA. This study highlights the potential of the LEAM system for use in various biomedical and biotechnological applications, owing to its effective handling of biomolecules.

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DNA Alignment-Induced Linear Dichroism via Magnetic Field Assisted Electrospray: Implications for Optical Applications

Cited by 2 publications

References 30 publications

Linear Dichroism Measurements for the Study of Protein-DNA Interactions

Linear Dichroism Measurements for the Study of Protein-DNA Interactions

Liquid Metal Electrodynamic Accumulation Microfluidics System for DNA Memory and Liquid Biopsy

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