DNA adsorption measured with ultra-thin film organic field effect transistors

Stoliar, Pablo; Bystrenová, Eva; Quiroga, Santiago David; Annibale, Paolo; Facchini, M.; Spijkman, Mark-Jan; Setayesh, Sepas; Leeuw, Dago de; Biscarini, Fabio

doi:10.1016/j.bios.2009.02.003

Cited by 73 publications

(62 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Organic thin film transistors (OTFT) hosting antibodies, peptides or enzymes have been developed for applications in aqueous and physiological media and have been studied extensively in the past [1-5, 7, 12-17]. The bio-recognition elements can be either attached to the surface of the gate in a top-gate configuration ( Figure 1a) [2-3, 5, 7, 18]; or on the surface of the semiconductor in a bottom gate structure using a solid insulator layer ( Figure 1b) [19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

On the mode of operation in electrolyte-gated thin film transistors based on different substituted polythiophenes

Toss¹,

Suspène²,

Piro³

et al. 2014

Organic Electronics

View full text Add to dashboard Cite

Organic Thin Film Transistors (OTFT), gated through an aqueous electrolyte, have extensively been studied as sensors in various applications. These water-gated devices are known to work both as electrochemical (Organic ElectroChemical Transistor - OECT) and field-effect (Organic Field-Effect Transistor - OFET) devices. To properly model and predict the response of water-gated OTFT sensors it is important to distinguish between the mechanism, field-effect or electrochemical, by which the transistor is modulated and thus how the gate signal can be affected by the analyte. In this present study we explore three organic polymer semiconductors, poly-(3-hexyl-thiophene) (P3HT), poly-(3-carboxypentyl-thiphene) (P3CPT) and a co-polymer P3HT-co-poly-(3-ethoxypentanoic acid-thiophene) (monomer ratio 1:6, P3HT-COOH15) in water-gated OTFT structures. We report a set of transistor characteristics, including standard output parameters, impedance spectroscopy and current transients, to investigate the origin of the mode of operation in these water-gated OTFTs. Impedance characteristics, including both frequency and voltage dependence, were recorded for capacitor stacks corresponding to the gate/electrolyte/semiconductor/source structure. It is shown that P3HT as well as P3HT-COOH15 both can function as semiconductors in water gated OTFT devices operating in field-effect mode. P3CPT on the other hand shows typical signs of electrochemical mode of operation. The -COOH side group has been suggested as a possible anchoring site for biorecognition elements in EGOFET sensors, rendering P3HT-COOH15 a possible candidate for such applications.

Funding Agencies|European Union [248728]; Swedish Government (SFO-AFM); Onnersjo Foundation (Holmen); Knut and Alice Wallenberg Foundation (Power Papers); VINNOVA (PEA)

show abstract

Section: Introductionmentioning

confidence: 99%

On the mode of operation in electrolyte-gated thin film transistors based on different substituted polythiophenes

Toss¹,

Suspène²,

Piro³

et al. 2014

Organic Electronics

View full text Add to dashboard Cite

Funding Agencies|European Union [248728]; Swedish Government (SFO-AFM); Onnersjo Foundation (Holmen); Knut and Alice Wallenberg Foundation (Power Papers); VINNOVA (PEA)

show abstract

“…In this novel hybrid system graphene could be used as the electrical interconnect and as an e-beam tailored substrate, while origami can be used as template for material growth, 26,27 sensor, 28,29 or even as a template for specific single-molecule chemical reactions. 30 …”

Section: Introductionmentioning

confidence: 99%

ssDNA Binding Reveals the Atomic Structure of Graphene

et al. 2010

View full text Add to dashboard Cite

We used AFM to investigate the interaction of polyelectrolytes such as ssDNA and dsDNA molecules with graphene as a substrate. Graphene is an appropriate substrate due to its planarity, relatively large surfaces that are detectable via an optical microscope, and straightforward identification of the number of layers. We observe that in the absence of the screening ions deposited ssDNA will bind only to the graphene and not to the SiO 2 substrate, confirming that the binding energy is mainly due to the π-π stacking interaction. Furthermore, deposited ssDNA will map the graphene underlying structure. We also quantify the π-π stacking interaction by correlating the amount of deposited DNA with the graphene layer thickness. Our findings agree with reported electrostatic force microscopy (EFM) measurements. Finally, we inspected the suitability of using a graphene as a substrate for DNA origami-based nanostructures.

show abstract

“…Consequently DNA is used for gene therapy, biological sensor preparation, tumor targeting delivery host for targeted drugs, gene and functional nanoscale electronic device, programmable DNAdirected self-assembly lm and DNA-conjugated metal nanoparticles. [8][9][10][11][12][13][14][15] Besides DNA chips and DNA microarrays are used in molecular biology, pharmaceutical industry and clinical research to identify presence of specic biological targets. 16 These kinds of technologies require pure DNA usage and understanding of DNA interaction with surfaces.…”

Section: Introductionmentioning

confidence: 99%

Calf thymus DNA characterization and its adsorption on different silica surfaces

Yetgin

Balköse

2015

RSC Adv.

View full text Add to dashboard Cite

DNA adsorption is the initial stage of gene therapy for drug delivery systems and solid phase extraction methods of DNA purification. High pore volume and high adsorption capacity are simple requirements not only for producing 'smart' drug delivery systems but also the development of purification kits. Silica is the most used material for this purpose. The present study aimed at elucidating the calf thymus DNA biosorption process by the characterization of calf thymus DNA and silica to increase the efficiency of the currently used silica material. Mesoporous silica has long been used for DNA adsorption and silica aerogel is the new adsorbent investigated in the present study. When DNA solution was freeze dried on a silica wafer, self-assembled super helices formed as shown by atomic microscopy (AFM). Thus DNA existed not as single molecules but as large sized agglomerates in water. Thus it could be adsorbed in the macropores and on the external surface of adsorbents. Adsorption of calf thymus DNA to a silica aerogel, a mesoporous silica gel and a silica wafer was investigated in the present study. Silica aerogel was synthesized from TEOS by a supercritical ethanol drying process. The DNA adsorption capacity of the silica aerogel was nearly two times that of the mesoporous silica gel due to its macroporous structure and its higher silanol content. Silica aerogel was found to be a very promising material for DNA adsorption. Therefore silica aerogel can be considered as a good candidate for the delivery of DNA.

show abstract

DNA adsorption measured with ultra-thin film organic field effect transistors

Cited by 73 publications

References 16 publications

On the mode of operation in electrolyte-gated thin film transistors based on different substituted polythiophenes

On the mode of operation in electrolyte-gated thin film transistors based on different substituted polythiophenes

ssDNA Binding Reveals the Atomic Structure of Graphene

Calf thymus DNA characterization and its adsorption on different silica surfaces

Contact Info

Product

Resources

About