DNA adsorbed on hydroxyapatite surfaces

Valle, Luís J. del; Bertrán, Oscar; Chaves, Gustavo; Revilla-López, Guillem; Rivas, Manuel; Casas, María Teresa; Casanovas, Jordi; Turón, Pau; Puiggalı́, Jordi; Alemán, Carlos

doi:10.1039/c4tb01184h

Cited by 42 publications

(58 citation statements)

References 57 publications

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“…Thus, biomineralization would facilitate DNA protection by the surrounding inorganic materials, while DNA transported at the surface of the mineral is attacked by endonucleases. [21] From the mass extinction events perspective, we recently discussed the formation and resilience of HOli particles that might made the difference for live surviving. [3] Results from this work also suggests that HOli particles when dissolving can be also viewed as nanocontainers that store and preserve DNA or RNA from changing environmental conditions, which may have many applications in biomedicine and genetics biotechnology.…”

Section: Resultsmentioning

confidence: 99%

Dissolving Hydroxyolite: A DNA Molecule into Its Hydroxyapatite Mold

Bertrán

Revilla-López

Casanovas

et al. 2016

Chemistry A European J

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Abstract:In spite of the clinical importance of hydroxyapatite (HAp), the mechanism that controls its dissolution in acidic environments remains unclear. Knowledge of such process is highly desirable to provide better understanding of different pathologies, as for example osteoporosis, and of the HAp potential as vehicle for gene delivery to replace damaged DNA. In this work, the mechanism of dissolution in acid conditions of HAp nanoparticles encapsulating double-stranded DNA has been investigated at the atomistic level using computer simulations. For this purpose, four consecutive (multi-step) molecular dynamics simulations, involving different temperatures and proton transfer processes, have been carried out. Results are consistent with a polynuclear decalcification mechanism in which proton transfer processes, from the surface to the internal regions of the particle, play a crucial role. Besides, the DNA remains protected by the mineral mold and transferred proton from both temperature and chemicals. These results, which indicate that biomineralization imparts very effective protection to DNA, also have important implications in other biomedical fields, as for example in the design of artificial bones or in the fight against osteoporosis by promoting the fixation of Ca 2+ ions.

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Section: Resultsmentioning

confidence: 99%

Dissolving Hydroxyolite: A DNA Molecule into Its Hydroxyapatite Mold

Bertrán

Revilla-López

Casanovas

et al. 2016

Chemistry A European J

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“…Additionally, we note that the nature of DNA-HAp interaction on the crystal surface depends on the exposed face of the crystal (Figure 3). [8] The binding on (001) face, the most Travelling back to the very origin of life it is worth noting that the finding of the first abiotic carbon was reported in 1996 by Mojzsis and coworkers [15] when they identified a 13 C-depleted microscopic graphite inclusion inside apatite dated 3,830 million years old in Akilia (Greenland). It was interpreted as a possible biomarker indicating the early connection between apatites and life.…”

Section: Hydroxyolitementioning

confidence: 99%

“…As a consequence, HAp adsorbs or encapsulates DNA and thus protects the biomolecule from external attacks. [8] In this paper, we refer to this particle as…”

Section: Introductionmentioning

confidence: 99%

Surviving Mass Extinctions through Biomineralized DNA

Turón

Puiggalı́

Bertrán

et al. 2015

Chemistry A European J

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Even in the worst of conditions, such as those which occurred during mass extinction events, life on Earth never totally stopped. Aggressive chemical and physical attacks able to sterilize or poison living organisms occurred repeatedly. Surprisingly, DNA was not degraded, denatured or modified to the point of losing the capability of transferring the genetic information to the next generations. After the events of mass extinction life was able to survive and thrive. DNA was passed on despite being an extremely fragile biomolecule. The potential implications of hydroxyapatite protection of DNA are discussed including how DNA acts as a template for HAp formation, how cell death can trigger biomineralizatoin, and how DNA can be successfully released from HAp when the conditions are favourable for life.

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“…PO . In a recent study 41 we found that the binding of DNA is disfavored for the latter termination. In this study we have only considered the termination with OH -14 and Ca 2+ ions for the (100) surface.…”

Section: Theoretical Characterization Of Orthophosphate Adsorption Onmentioning

confidence: 99%

“…Also, HAp nanoparticles are suitable as gene delivery systems for the transfection of cells with nucleic acids. [39][40][41] Thus, HAp confers protection to DNA from chemical and enzymatic degradation, increasing the stability of the biomolecule. 41,42 On the other hand, the BPs the oxygen atom that links the phosphate groups of pyrophosphates is replaced by a carbon atom.…”

Section: O Pmentioning

confidence: 99%

An experimental-computer modeling study of inorganic phosphates surface adsorption on hydroxyapatite particles

et al. 2015

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The adsorption of orthophosphate, pyrophosphate, triphosphate and a trisphosphonate onto hydroxyapatite has been examined using experiments and quantum mechanical calculations. Adsorption studies with FTIR and X-ray photoelectron spectroscopies have been performed considering both crystalline hydroxyapatite (HAp) and amorphous calcium phosphate particles, which were specifically prepared and characterized for this purpose. Density Functional Theory (DFT) calculations have been carried out considering the (100) and (001)

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DNA adsorbed on hydroxyapatite surfaces

Cited by 42 publications

References 57 publications

Dissolving Hydroxyolite: A DNA Molecule into Its Hydroxyapatite Mold

Dissolving Hydroxyolite: A DNA Molecule into Its Hydroxyapatite Mold

Surviving Mass Extinctions through Biomineralized DNA

An experimental-computer modeling study of inorganic phosphates surface adsorption on hydroxyapatite particles

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