DNA adenine methylation of GATC sequences appeared recently in the Escherichia coli lineage

Barbeyron, Tristan; Kean, Katherine M.; Forterre, Patrick

doi:10.1128/jb.160.2.586-590.1984

Cited by 96 publications

(22 citation statements)

References 31 publications

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“…Corresponding to previous studies on DNA methylation by restriction analysis (15), it was concluded that in S. marcescens Sr41, the GATC sequences are methylated as in E. coli by the Dam enzyme (28). This corresponds to a previous finding of dam methylation in S. marcescens (5). Since it is known that dam mutants of E. coli are sensitive to 2-AP (17), we decided to use 2-AP sensitivity in screening for a dam mutant of S. marcescens.…”

Section: Isolation Of a Dam Mutant Preliminary Experiments Hadsupporting

confidence: 56%

Characterization of a dam Mutant of Serratia marcescens and Nucleotide Sequence of the dam Region

et al. 1999

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The DNA of Serratia marcescens has N6-adenine methylation in GATC sequences. Among 2-aminopurine-sensitive mutants isolated from S. marcescensSr41, one was identified which lacked GATC methylation. The mutant showed up to 30-fold increased spontaneous mutability and enhanced mutability after treatment with 2-aminopurine, ethyl methanesulfonate, or UV light. The gene (dam) coding for the adenine methyltransferase (Dam enzyme) of S. marcescens was identified on a gene bank plasmid which alleviated the 2-aminopurine sensitivity and the higher mutability of adam-13::Tn9 mutant ofEscherichia coli. Nucleotide sequencing revealed that the deduced amino acid sequence of Dam (270 amino acids; molecular mass, 31.3 kDa) has 72% identity to the Dam enzyme of E. coli. The dam gene is located between flanking genes which are similar to those found to the sides of the E. coli damgene. The results of complementation studies indicated that like Dam ofE. coli and unlike Dam of Vibrio cholerae, the Dam enzyme of S. marcescens plays an important role in mutation avoidance by allowing the mismatch repair enzymes to discriminate between the parental and newly synthesized strands during correction of replication errors.

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Section: Isolation Of a Dam Mutant Preliminary Experiments Hadsupporting

confidence: 56%

Characterization of a dam Mutant of Serratia marcescens and Nucleotide Sequence of the dam Region

et al. 1999

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“…Thus, it may be possible to trace the evolution of metabolic pathways in procaryotes to an extent never before thought possible. Some good examples are the recent investigations on the regulation of aromatic amino acid biosynthesis and DNA adenine methylation (16,48).…”

Section: Discussionmentioning

confidence: 99%

Methanogens and the diversity of archaebacteria.

Jones¹,

Nagle²,

Whitman³

1987

Microbiological Reviews

291

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TABLE 1. Summary of characteristics of methanogenic archaebacteria, order Methanobacterialesa Temp p Archaebacteria Morphology Substrates G+C optimum pH Cell envelope Major membrane Reference(s) (mol%) (OC) optimum composition isoprenoid Family Methanobacteriaceae Methanobacterium formicicum Rod H2, formate 40.7 37 7.0 Pseudomurein C20 + C40 12 M. bryantii Rod H2 32.7 38 7.0 Pseudomurein C20 + C40 12 M. thermoautotrophicum Rod H,

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“…Unlike 5mC, 6mA, and 4mC are found at significant levels in the genomic DNA of bacteria, protists, fungi, and eukaryotes . Particularly in bacteria, 6mA plays an important role in controlling a number of biological functions, such as DNA replication , cell cycle progression , DNA mismatch repair , host‐pathogen interactions , and virulence and gene expression .…”

Section: Introductionmentioning

confidence: 99%

N⁶‐methyladenine functions as a potential epigenetic mark in eukaryotes

et al. 2015

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N(6)-methyladenine (6mA) is one of the most abundant types of DNA methylation, and plays an important role in bacteria; however, its roles in higher eukaryotes, such as plants, insects, and mammals, have been considered less important. Recent studies highlight that 6mA does indeed occur, and that it plays an important role in eukaryotes, such as worm, fly, and green algae, and thus the regulation of 6mA has emerged as a novel epigenetic mechanism in higher eukaryotes. Despite this intriguing development, a number of important issues regarding its biological roles are yet to be addressed. In this review, we focus on the 5mC and 6mA modifications in terms of their production, distribution, and the erasure of 6mA in higher eukaryotes including mammals. We perform an analysis of the potential functions of 6mA, hence widening understanding of this new epigenetic mark in higher eukaryotes, and suggesting future studies in this field.

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DNA adenine methylation of GATC sequences appeared recently in the Escherichia coli lineage

Abstract: We have examined the presence of methylated adenine at GATC sequences (Dam phenotype) in the DNA of 23 eubacteria and 13 archaebacteria by using isoshizomer restriction enzymes. We have found a completely

Cited by 96 publications

References 31 publications

Characterization of a dam Mutant of Serratia marcescens and Nucleotide Sequence of the dam Region

Characterization of a dam Mutant of Serratia marcescens and Nucleotide Sequence of the dam Region

Methanogens and the diversity of archaebacteria.

N⁶‐methyladenine functions as a potential epigenetic mark in eukaryotes

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DNA adenine methylation of GATC sequences appeared recently in the Escherichia coli lineage

Abstract: We have examined the presence of methylated adenine at GATC sequences (Dam phenotype) in the DNA of 23 eubacteria and 13 archaebacteria by using isoshizomer restriction enzymes. We have found a completely

Cited by 96 publications

References 31 publications

Characterization of a dam Mutant of Serratia marcescens and Nucleotide Sequence of the dam Region

Characterization of a dam Mutant of Serratia marcescens and Nucleotide Sequence of the dam Region

Methanogens and the diversity of archaebacteria.

N6‐methyladenine functions as a potential epigenetic mark in eukaryotes

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N⁶‐methyladenine functions as a potential epigenetic mark in eukaryotes