DNA Acts as a Nucleation Site for Transient Cavitation in the Ultrasonic Nebulizer

Lentz, Yvonne K.; Anchordoquy, Thomas J.; Lengsfeld, Corinne S.

doi:10.1002/jps.20511

Cited by 29 publications

(25 citation statements)

References 58 publications

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“…PEI is cationic in nature and has strong DNA compaction capacity, effective DNA protection. The plasmid DNA with negative charge could be protected from degradation by PEI during mechanical process [19] . PEI plays an important role in the process of transfection.…”

Section: Discussionmentioning

confidence: 99%

Efficient gene delivery to myocardium with ultrasound targeted microbubble destruction and polyethylenimine

Chen

Xie

Wang

et al. 2008

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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The aim of present study was to evaluate the feasibility and efficiency of enhanced green fluorescent protein (EGFP) gene delivery to myocardium in vivo by ultrasound targeted microbubble destruction (UTMD) and polyethylenimine (PEI). SonoVue/DNA and PEI/DNA/SonoVue complexes were prepared. Gel electrophoresis analysis was performed to determine the structural integrity of plasmid DNA or PEI/DNA after UTMD. Solutions of plasmid DNA, SonoVue/DNA, PEI/DNA complexes or PEI/DNA/SonoVue complexes were respectively transduced into BALB/c mice hearts by means of transthoracic ultrasound irradiation. Mice undergoing PBS injection, plasmid injection or PEI/DNA complexes injection without ultrasound irradiation served as controls. Gene expression in myocardium was detected 4 days after treatment. Cryosections and histological examinations were conducted. Electrophoresis gel assay showed no damage to DNA or PEI/DNA complexes after UTMD. When the heart was not exposed to ultrasound, the expression of EGFP was observed in the subendocardial myocardium obviously. The strongest expression was detected in the anterior wall of the left ventricle when the heart was exposed to ultrasound alone. Injection of PEI/DNA complexes and UTMD resulted in the highest transfection efficiency and the distributional difference of EGFP was not obvious. No tissue damage was seen histologically. In conclusion, a combination of UTMD and PEI was highly effective in transfecting mice hearts without causing any apparently adverse effect. It provides an alternative to current clinical gene therapy and opens a new concept of non-viral gene delivery for the treatment of cardiac disease.

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Section: Discussionmentioning

confidence: 99%

Efficient gene delivery to myocardium with ultrasound targeted microbubble destruction and polyethylenimine

Chen

Xie

Wang

et al. 2008

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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“…Our previous studies have shown cavitation, not shear, is responsible for plasmid and cosmid DNA degradation in this nebulizer. (81) Specifically, DNA, functioning as an impurity within the solution, acts a nucleation site for cavitation. Upon the collapse of a bubble, the DNA is destroyed as a result of the newly created shock waves and areas of high radical concentrations, temperatures and pressures.…”

Section: Discussionmentioning

confidence: 99%

Rationale for the Selection of an Aerosol Delivery System for Gene Delivery

Lentz

Anchordoquy

Lengsfeld

2006

Journal of Aerosol Medicine

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Genetic therapeutics show great promise toward the treatment of illnesses associated with the lungs; however, current methods of delivery such as jet and ultrasonic nebulization decrease the activity and effectiveness of these treatments. Extremely low transfection rates exhibited by non-complexed plasmid DNA in these nebulizers have been primarily attributed to poor translocation and loss of molecular integrity as a consequence of shear-induced degradation. Current research focusing on methods to increase transfection rates via the pulmonary delivery route has largely concentrated on the incorporation of carbon dioxide in the air stream to increase breath depth as well as the addition of cationic agents that condense DNA into compact, ordered complexes. The purpose of this study was to examine the impact of several classic as well as the latest atomization devices on the structure of non-complexed DNA. Various sizes of plasmid and cosmid DNA were processed through an electrostatic spray, ultrasonic nebulizer, vibrating mesh nebulizer, and jet nebulizer. Results varied dramatically based upon atomization device as well as DNA size. This may explain the inefficiency experienced by genetic therapeutics during pulmonary delivery. More importantly, this suggests that the selection of an atomization device should consider DNA size in order to achieve optimal gene delivery to the lungs.

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“…Both were followed by DNA extractionanalysis using Qiagen EZ1 investigator kit (Qiagen, Crawley, UK) and PCR [42]. The ultrasonic power level (30 W) was chosen to be insufficient to degrade or cleave DNA [43,44].…”

Section: Nanoabrasivesmentioning

confidence: 99%