DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality

Ynsaurralde-Rivolta, A. E.; Suvá, M.; Luchetti, Carolina Griselda; Bevacqua, R. J.; Munilla, S.; Rodríguez-Álvarez, L.; Velásquez, Alejandra; Briski, O.; Lombardo, Daniel Marcelo; Salamone, D.

doi:10.1016/j.theriogenology.2020.02.045

Cited by 13 publications

(3 citation statements)

References 64 publications

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“…However, no significant ( p > 0.05) difference was observed in the developmental competence of PA embryos between the control and NT-4-treated groups. In a bovine study, 0.5% DMSO supplementation during IVM increased first polar body extrusion rate and improved subsequent embryonic development after IVF ( Ynsaurralde-Rivolta et al, 2020 ). In pigs, short- (20 h) or long-term (144 h) exposure to DMSO, a vehicle at low concentrations (0.1%), was not toxic to the development of in vitro -produced IVF embryos ( Lucas et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Neurotrophin-4 promotes the specification of trophectoderm lineage after parthenogenetic activation and enhances porcine early embryonic development

Kim

Lee

Cai

et al. 2023

Front. Cell Dev. Biol.

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Neurotrophin-4 (NT-4), a neurotrophic factor, appears to affect early embryonic development because it is secreted not only by neurons but also by oviductal and uterine epithelial cells. However, no studies have characterized the effects of NT-4 on early embryonic development in pigs. In this study, we applied the experimental model of parthenogenetic-activation (PA)-derived embryos. Herein, we investigated the effect of NT-4 supplementation during the in vitro culture (IVC) of embryos, analyzed the transcription levels of specific genes, and outlined the first cell lineage specification for porcine PA-derived blastocysts. We confirmed that NT-4 and its receptor proteins were localized in both the inner cell mass (ICM) and trophectoderm (TE) in porcine blastocysts. Across different concentrations (0, 1, 10, and 100 ng/mL) of NT-4 supplementation, the optimal concentration of NT-4 to improve the developmental competence of porcine parthenotes was 10 ng/mL. NT-4 supplementation during porcine IVC significantly (p < 0.05) increased the proportion of TE cells by inducing the transcription of TE lineage markers (CDX2, PPAG3, and GATA3 transcripts). NT-4 also reduced blastocyst apoptosis by regulating the transcription of apoptosis-related genes (BAX and BCL2L1 transcripts) and improved blastocyst quality via the interaction of neurotrophin-, Hippo-yes-associated protein (Hippo-YAP) and mitogen-activated protein kinase/extracellular regulated kinase (MAPK/ERK) pathway. Additionally, NT-4 supplementation during IVC significantly (p < 0.05) increased YAP1 transcript levels and significantly (p < 0.01) decreased LATS2 transcript levels, respectively, in the porcine PA-derived blastocysts. We also confirmed through fluorescence intensity that the YAP1 protein was significantly (p < 0.001) increased in the NT-4-treated blastocysts compared with that in the control. NT-4 also promoted differentiation into the TE lineage rather than into the ICM lineage during porcine early embryonic development. In conclusion, 10 ng/mL NT-4 supplementation enhanced blastocyst quality by regulating the apoptosis- and TE lineage specification-related genes and interacting with neurotrophin-, Hippo-YAP-, and MAPK/ERK signaling pathway during porcine in vitro embryo development.

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Section: Discussionmentioning

confidence: 99%

Neurotrophin-4 promotes the specification of trophectoderm lineage after parthenogenetic activation and enhances porcine early embryonic development

Kim

Lee

Cai

et al. 2023

Front. Cell Dev. Biol.

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“…DMSO is regularly used in media for the production and the cryopreservation of bovine embryos [9,14,38,39]. Due to its cellular toxicity and its impact on the maintenance of pluripotency [40], DMSO 21 should only be used at a low concentration [41], which was the case in the present study (0.02 or 0.1%).…”

Section: Limitations Of the Studymentioning

confidence: 91%

Effect of DHA on the quality of In vitro produced bovine embryos

et al. 2022

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“…On average, blastocyst rates (blastocyst/oocyte) of IVP embryos at day seven or eight post-fertilization usually fluctuates between 30 and 40 percent [ 2 – 5 ]. Various factors and protein sources have been used to supplement IVP media in order to promote embryo development in vitro [ 6 – 11 ]. For example, bovine serum (e.g.…”

Section: Introductionmentioning

confidence: 99%

Serum supplementation during bovine embryo culture affects their development and proliferation through macroautophagy and endoplasmic reticulum stress regulation

et al. 2021

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Serum supplementation during bovine embryo culture has been demonstrated to promote cell proliferation and preimplantation embryo development. However, these desirable outcomes, have been associated with gene expression alterations of pathways involved in macroautophagy, growth, and development at the blastocyst stage, as well as with developmental anomalies such as fetal overgrowth and placental malformations. In order to start dissecting the molecular pathways by which serum supplementation of the culture medium during the preimplantation stage promotes developmental abnormalities, we examined blastocyst morphometry, inner cell mass and trophectoderm cell allocations, macroautophagy, and endoplasmic reticulum stress. On day 5 post-insemination, > 16 cells embryos were selected and cultured in medium containing 10% serum or left as controls. Embryo diameter, inner cell mass and trophectoderm cell number, and macroautophagy were measured on day 8 blastocysts (BL) and expanded blastocysts (XBL). On day 5 and day 8, we assessed transcript level of the ER stress markers HSPA5, ATF4, MTHFD2, and SHMT2 as well as XBP1 splicing (a marker of the unfolded protein response). Serum increased diameter and proliferation of embryos when compared to the no-serum group. In addition, serum increased macroautophagy of BL when compared to controls, while the opposite was true for XBL. None of the genes analyzed was differentially expressed at any stage, except that serum decreased HSPA5 in day 5 > 16 cells stage embryos. XBP1 splicing was decreased in BL when compared to XBL, but only in the serum group. Our data suggest that serum rescues delayed embryos by alleviating endoplasmic reticulum stress and promotes development of advanced embryos by decreasing macroautophagy.

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DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality

Cited by 13 publications

References 64 publications

Neurotrophin-4 promotes the specification of trophectoderm lineage after parthenogenetic activation and enhances porcine early embryonic development

Neurotrophin-4 promotes the specification of trophectoderm lineage after parthenogenetic activation and enhances porcine early embryonic development

Effect of DHA on the quality of In vitro produced bovine embryos

Serum supplementation during bovine embryo culture affects their development and proliferation through macroautophagy and endoplasmic reticulum stress regulation

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