DMSO induces apoptosis in SV40-transformed human keratinocytes, but not in normal keratinocytes

Koike, Manabu; Ishino, Keiko; Kohno, Yohko; Tachikawa, Tetsuhiko; Kartašova, Tonja; Kuroki, Toshio; Huh, Nam-ho

doi:10.1016/s0304-3835(96)04408-4

Cited by 23 publications

(13 citation statements)

References 17 publications

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“…Because DMSO has been reported to induce apoptosis, for example, in human leukemic U937 cells [23,24], SV40-transformed human keratinocytes [25], or thymic lymphoma cells [26], we ® rst investigated whether DMSO alone would affect the mRNA expression of ENaC and Na ‡ -K ‡ -ATPase. When alveolar type II cells were stimulated with 100 nM PMA, the ® nal concentration of DMSO in each well was 0.1%.…”

Section: Resultsmentioning

confidence: 99%

The Impact of Phorbol Ester on the Regulation of Amiloride-Sensitive Epithelial Sodium Channel in Alveolar Type Ii Epithelial Cells

Yamagata

Nishimoto

et al. 2002

Experimental Lung Research

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Amiloride-sensitive sodium channel (ENaC) plays an important role in recovery from pulmonary edema. Recently, it has been shown that an activation of protein kinase C (PKC) could affect the mRNA expression of ENaC in rat parotid gland cells and A6 distal nephron epithelial cells. To determine whether an activation of PKC would regulate the mRNA expression or the function of ENaC, we stimulated rat alveolar type II epithelial cells with phorbol 12-myristate 13-acetate (PMA), a potent PKC activator, at a concentration of 100 nM. The mRNA expression of alpha-, beta-, and gamma-ENaC subunits and amiloride-sensitive current were measured. PMA inhibited the mRNA expression of all 3 ENaC subunits (alpha-ENaC: 56.0% +/- 12.1%; beta-ENaC: 62.6% +/- 15.9%; gamma-ENaC: 68.5% +/- 10.6%, respectively) and amiloride-sensitive current (control = 7.0 +/- 1.5 microA/cm(2); PMA = 1.7 +/- 0.9 microA/cm(2)) significantly at 24 hours. On the other hand, 4alpha-phorbol didecanoate 4alpha-PDD, inactive form of PMA, had no inhibitory effect on alpha- and gamma-ENaC expression or amiloride-sensitive current. However, no significant difference was seen in beta-ENaC expression between PMA and 4alpha-PDD. GF 109203X, a wide-range PKC inhibitor, blocked the inhibitory effect of PMA on all ENaC subunits mRNA expression. These results suggest that an activation of PKC may play an important role in the regulation of ENaC mRNA expression and function.

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Section: Resultsmentioning

confidence: 99%

The Impact of Phorbol Ester on the Regulation of Amiloride-Sensitive Epithelial Sodium Channel in Alveolar Type Ii Epithelial Cells

Yamagata

Nishimoto

et al. 2002

Experimental Lung Research

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“…Immunoprecipitation was performed as previously described (Koike et al, 1996b). The Ku products were immunoprecipitated with the above-mentioned antibodies (anti-Ku70 monoclonal antibody (N3H10) or rabbit anti-Ku80 polyclonal antibody (AHP317)) in combination with protein ASepharose (Pharmacia, Uppsala, Sweden).…”

Section: Immunoprecipitationmentioning

confidence: 99%

Ku80 can translocate to the nucleus independent of the translocation of Ku70 using its own nuclear localization signal

Koike¹,

Ikuta

Miyasaka³

et al. 1999

Oncogene

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Ku antigen is a complex of Ku70 and Ku80 subunits and plays an important role in not only DNA double-strand breaks (DSB) repair and V(D)J recombination, but also in growth regulation. Ku is generally believed to always form and function as heterodimers on the basis of in vitro observations. Here we demonstrate that the localization of Ku80 does not completely coincide with that of Ku70. Ku70 and Ku80 were colocalized in the nucleus in the interphase but not in the late telophase/early G1 phase of the cell cycle. Since the in vivo function of Ku might be partially regulated by the control of its transport, we attempted to investigate the molecular mechanisms underlying the nuclear translocation of Ku. The nuclear translocation of Ku80 started during the late telophase/ early G1 phase after the nuclear envelope was formed and this was preceded by the nuclear translocation of Ku70. Furthermore, we found that the Ku80 protein was transported to the nucleus without heterodimerization with Ku70. To understand in detail the mechanism of transport of Ku80, we attempted to identify the nuclear localization signal (NLS) of Ku80 and de®ned to a region spanning nine amino acid residues (positions 561 ± 569). The Ku80 NLS was demonstrated to be mediated to the nuclear rim by two components of PTAC58 and PTAC97. All these ®ndings support the idea that Ku80 can translocate to the nucleus using its own NLS independent of the translocation of Ku70.

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“…Moreover, liposomes rapidly penetrate the dermal barrier, making the delivery of water-soluble compounds as effective as that of lipid-soluble compounds (24). In contrast with other drug carriers such as DMSO (25), liposomes are entirely non-toxic to human skin since they are composed of naturally occurring biodegradable compartments (26). …”

Section: Introductionmentioning

confidence: 99%

Nanoliposomes of L-lysine-conjugated poly(aspartic acid) Increase the Generation and Function of Bone Marrow-derived Dendritic Cells

Kim

Ji³

et al. 2011

Immune Netw

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BackgroundBiodegradable polymers have increasingly been recognized for various biological applications in recent years. Here we examined the immunostimulatory activities of the novel poly(aspartic acid) conjugated with L-lysine (PLA).MethodsPLA was synthesized by conjugating L-lysine to aspartic acid polymer. PLA-nanoliposomes (PLA-NLs) were prepared from PLA using a microfluidizer. The immunostimulatory activities of PLA-NLs were examined in mouse bone marrow-derived dendritic cells (BM-DCs).ResultsPLA-NLs increased the number of BM-DCs when added to cultures of GM-CSF-induced DC generation on day 4 after the initiation of cultures. Examination of the phenotypic properties showed that BM-DCs generated in the presence of PLA-NLs are more mature in terms of the expression of MHC class II molecules and major co-stimulatory molecules than BM-DCs generated in the absence of PLA-NLs. In addition, the BM-DCs exhibited enhanced capability to produce cytokines, such as IL-6, IL-12, TNF-α and IL-1β. Allogeneic mixed lymphocyte reactions also confirmed that the BMDCs were more stimulatory on allogeneic T cells. PLA- NL also induced further growth of immature BM-DCs that were harvested on day 8.ConclusionThese results show that PLA-NLs induce the generation and functional activities of BM-DCs, and suggest that PLA-NLs could be immunostimulating agents that target DCs.

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DMSO induces apoptosis in SV40-transformed human keratinocytes, but not in normal keratinocytes

Cited by 23 publications

References 17 publications

The Impact of Phorbol Ester on the Regulation of Amiloride-Sensitive Epithelial Sodium Channel in Alveolar Type Ii Epithelial Cells

The Impact of Phorbol Ester on the Regulation of Amiloride-Sensitive Epithelial Sodium Channel in Alveolar Type Ii Epithelial Cells

Ku80 can translocate to the nucleus independent of the translocation of Ku70 using its own nuclear localization signal

Nanoliposomes of L-lysine-conjugated poly(aspartic acid) Increase the Generation and Function of Bone Marrow-derived Dendritic Cells

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