DL-Myo-inositol 1,4,5-trisphosphorothioate mobilizes intracellular calcium in Swiss 3T3 cells and Xenopus oocytes

Taylor, Colin W.; Berridge, Michael J.; Brown, Kenneth D.; Cooke, Allan M.; Potter, Barry V. L.

doi:10.1016/0006-291x(88)90438-x

Cited by 49 publications

(17 citation statements)

References 18 publications

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“…This effect of Ins 1,3,4,5 P4 cannot be explained by interference with the metabolism of Ins 1,4,5 P3, since it is also seen when the trisphosphate is present in great excess of the tetrakisphosphate (Fig. 2) as well as when the stable analogue Ins 1,4,5 P(S)3 (Cooke et al, 1987;Taylor et al, 1988) is used (Fig. 3).…”

Section: Discussionmentioning

confidence: 88%

“…In other experiments Ins !,4,5 P3 (10/~M) was introduced first, giving a transient response, and thereafter 2,3 DPG was added on top. 2,3 DPG in these cases evoked a small transient response, but again Finally, the phosphatase-resistant analogue DLinositol 1,4,5-trisphosphorothioate (Ins 1,4,5 P(S)3) was used (Cooke et al, 1987;Hamblin, Flora & Potter, 1987;Taylor et al, 1988). This analogue, as well as being a potent releaser of calcium, is a much more effective 5-phosphatase inhibitor than 2,3 DPG and has a Ki of 6 /,ZM (Cooke, Nahorski & Potter, 1989).…”

Section: Is the Transient Response To Ins 145 P3 Stimulation Due Tomentioning

confidence: 97%

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Inositol 1,3,4,5-tetrakisphosphate is essential for sustained activation of the Ca2+-dependent K+ current in single internally perfused mouse lacrimal acinar cells

et al. 1989

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We have examined the effects of various inositol polyphosphates, alone and in combination, on the Ca2+-activated K+ current in internally perfused, single mouse lacrimal acinar cells. We used the patch-clamp technique for whole-cell current recording with a set-up allowing exchange of the pipette solution during individual experiments so that control and test periods could be directly compared in individual cells. Inositol 1,4,5-trisphosphate (Ins 1,4,5 P3) (10-100 microM) evoked a transient increase in the Ca2+-sensitive K+ current that was independent of the presence of Ca2+ in the external solution. The transient nature of the Ins 1,4,5 P3 effect was not due to rapid metabolic breakdown, as similar responses were obtained in the presence of 5 mM 2,3-diphosphoglyceric acid, that blocks the hydrolysis of Ins 1,4,5 P3, as well as with the stable analogue DL-inositol 1,4,5-trisphosphorothioate (Ins 1,4,5 P(S)3) (100 microM). Ins 1,3,4 P3 (50 microM) had no effect, whereas 50 microM Ins 2,4,5 P3 evoked responses similar to those obtained by 10 microM Ins 1,4,5 P3. A sustained increase in Ca2+-dependent K+ current was only observed when inositol 1,3,4,5-tetrakisphosphate (Ins 1,3,4,5 P4) (10 microM) was added to the Ins 1,4,5 P3 (10 microM)-containing solution and this effect could be terminated by removal of external Ca2+. The effect of Ins 1,3,4,5 P4 was specifically dependent on the presence of Ins 1,4,5 P3 as it was not found when 10 microM concentrations of Ins 1,3,4 P3 or Ins 2,4,5 P3 were used.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionmentioning

confidence: 88%

Section: Is the Transient Response To Ins 145 P3 Stimulation Due Tomentioning

confidence: 97%

Inositol 1,3,4,5-tetrakisphosphate is essential for sustained activation of the Ca2+-dependent K+ current in single internally perfused mouse lacrimal acinar cells

et al. 1989

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“…Release of sequestered intracellular Ca2+ is initiated in the native by a variety of hormones and neurotransmitters including acetylcholine [30], This Ca2+ mo bilization appears to involve activation of a phospholipase C which acts to break down polyphosphoinositides. It has been reported that acetylcholine acting on endogenous oo cyte muscarinic receptors brings about an increase in the inositol trisphosphate content [30], while injection of inositol trisphos phate into oocytes brought about release of Ca2+ from intracellular stores [31].…”

Section: Discussionmentioning

confidence: 99%

Expression of Ca<sup>2+</sup> Mobilizing Receptors in Xenopus Oocytes: A Tool for Receptor Characterization

Logsdon

Williams²,

Stuenkel

et al. 1990

Digestion

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In order to investigate the molecular characteristics of gastrointestinal hormone receptors, we have expressed their mRNAs in Xenopus laevis oocytes. Xenopus oocytes possess intrinsic muscarinic cholinergic receptors which couple to intracellular Ca²⁺ release. Release of intracellular Ca²⁺ was detected by an increase in ⁴⁵Ca²⁺ release from preloaded oocytes or by using the Ca²⁺-sensitive fluorescent dye fura-2. Similarly, cholecystokinin (CCK) receptors expressed on the surface of oocytes which were injected with raRNA prepared from rat pancreatic acinar AR42J cells were readily detected by their ability to mobilize intracellular Ca²⁺. The CCK receptors expressed in oocytes showed similar binding characteristics as the native receptors. CCK receptor expression in the oocytes could be specifically blocked by hybridizing the mRNA with antisense oligonucleotides based on the highly conserved second transmembrane region of the HM4 muscarinic cholinergic receptor before injection. These latter results strongly suggest that the CCK receptor is a member of the G protein-linked receptor family. Thus, expression in Xenopus laevis oocytes provides a powerful tool for elucidation of the molecular characteristics of gastrointestinal hormone receptors.

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“…DL-lns(1,4,5)P(S)3 was synthesized according to Cooke et al (1987) and was purified by ion-exchange chromatography. L-lns(1,4,5)P3 was inactive in causing oscillations when injected into Xenopus oocytes (Taylor et al, 1988).…”

Section: Methodsmentioning

confidence: 99%

“…The latter model emerged from work carried out on immature oocytes of Xenopus, which produce membrane potential oscillations in response to injections of lns(1 ,4,5)P3 (Oron et al, 1985;Berridge, 1988Berridge, , 1989 (Berridge, 1988). Using such high injection currents, there was little difference in the oscillatory response to Ins(1 ,4,5)P3 and lns(1 ,4,5)P(S)3 (Taylor et al, 1988 Figure 1, whereas in other cases the first transient after injection was small but then increased over the next few transients, as shown for the lns(1,4,5)P(S)3 responses in Figure 2. To determine whether there was any consistent difference between these two analogues, we loaded them into the separate halves of a double-barreled electrode so that they could be injected alternatively into the same location of a single oocyte (Figure 3).…”

Section: Introductionmentioning

confidence: 99%

Inositol trisphosphate analogues induce different oscillatory patterns in Xenopus oocytes.

Berridge¹,

Potter²

1990

Cell Regul

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Agonists that utilize the calcium-mobilizing second messenger inositol(1,4,5)trisphosphate Ins(1,4,5)P3 usually generate oscillations in intracellular calcium. Such oscillations, based on the periodic release of calcium from the endoplasmic reticulum, can also be induced by injecting cells with Ins(1,4,5)P3. The mechanism responsible for oscillatory activity was studied in Xenopus oocytes by injecting them with different inositol trisphosphates. The plasma membrane of Xenopus oocytes has calcium-dependent chloride channels that open in response to calcium, leading to membrane depolarization. Oscillations in calcium were thus monitored by recording membrane potential. The naturally occurring Ins(1,4,5)P3 produced a large initial transient followed by a single transient or a burst of oscillations. By contrast, two analogues (Ins(2,4,5)P3 and Ins(1,4,5)P(S)3) produced a different oscillatory pattern made up of a short burst of sharp transients. Ins(1,3,4,5)P4 had no effect when injected by itself, and it also failed to modify the oscillatory responses to either Ins(2,4,5)P3 or Ins(1,4,5)P(S)3. Both analogues failed to induce a response when injected immediately after the initial Ins(1,4,5)P3-induced response, indicating that they act on the same intracellular pool of calcium. The existence of different oscillatory patterns suggests that there may be different mechanisms for setting up calcium oscillations. The Ins(2,4,5)P3 and Ins(1,4,5)P(S)3 analogues may initiate oscillations through a negative feedback mechanism whereby calcium inhibits its own release. The two-pool model is the most likely mechanism to describe the Ins(1,4,5)P3-induced oscillations.

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DL-Myo-inositol 1,4,5-trisphosphorothioate mobilizes intracellular calcium in Swiss 3T3 cells and Xenopus oocytes

Cited by 49 publications

References 18 publications

Inositol 1,3,4,5-tetrakisphosphate is essential for sustained activation of the Ca2+-dependent K+ current in single internally perfused mouse lacrimal acinar cells

Inositol 1,3,4,5-tetrakisphosphate is essential for sustained activation of the Ca2+-dependent K+ current in single internally perfused mouse lacrimal acinar cells

Expression of Ca<sup>2+</sup> Mobilizing Receptors in Xenopus Oocytes: A Tool for Receptor Characterization

Inositol trisphosphate analogues induce different oscillatory patterns in Xenopus oocytes.

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