Dl-3-n-butylphthalide attenuates myocardial ischemia reperfusion injury by suppressing oxidative stress and regulating cardiac mitophagy via the PINK1/Parkin pathway in rats

Zhang, Dongqin; Zheng, Nan; Fu, Xiaoli; Shi, Jian; Zhang, Jun

doi:10.21037/jtd-22-585

Cited by 9 publications

(5 citation statements)

References 36 publications

(42 reference statements)

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“…42 A previous study reported that NBP could attenuate myocardial ischemia-reperfusion injury by inhibiting oxidative stress. 43 In addition, it has also been reported that NBP alleviated secondary brain injury by promoting neovascularization and vascular remodeling during recovery. 43 Given NBP's favorable properties of reducing oxidative stress levels and enhancing angiogenesis, we hypothesize that NBP may also prove beneficial in rapid TES healing.…”

Section: ■ Introductionmentioning

confidence: 99%

“…43 In addition, it has also been reported that NBP alleviated secondary brain injury by promoting neovascularization and vascular remodeling during recovery. 43 Given NBP's favorable properties of reducing oxidative stress levels and enhancing angiogenesis, we hypothesize that NBP may also prove beneficial in rapid TES healing. Building on this hypothesis, NBP and BP nanoflakes were both encapsulated in a chitosan (CS) hydrogel to determine whether their incorporation could be used for enhanced osteogenesis and angiogenesis.…”

Section: ■ Introductionmentioning

confidence: 99%

“…It has been approved by the China Food and Drug Administration for the treatment of stroke in China since 2002 . A previous study reported that NBP could attenuate myocardial ischemia-reperfusion injury by inhibiting oxidative stress . In addition, it has also been reported that NBP alleviated secondary brain injury by promoting neovascularization and vascular remodeling during recovery .…”

Section: Introductionmentioning

confidence: 99%

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An Injectable Black Phosphorus Hydrogel for Rapid Tooth Extraction Socket Healing

Guo,

Li,

Chen

et al. 2024

ACS Appl. Mater. Interfaces

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The excess production of reactive oxygen species (ROS) will delay tooth extraction socket (TES) healing. In this study, we developed an injectable thermosensitive hydrogel (NBP@BP@CS) used to treat TES healing. The hydrogel formulation incorporated black phosphorus (BP) nanoflakes, recognized for their accelerated alveolar bone regeneration and ROS-scavenging properties, and dl-3-n-butylphthalide (NBP), a vasodilator aimed at enhancing angiogenesis. In vivo investigations strongly demonstrated that NBP@BP@CS improved TES healing due to antioxidation and promotion of alveolar bone regeneration by BP nanoflakes. The sustained release of NBP from the hydrogel promoted neovascularization and vascular remodeling. Our results demonstrated that the designed thermosensitive hydrogel provided great opportunity not only for ROS elimination but also for the promotion of osteogenesis and angiogenesis, reflecting the "three birds with one stone" concept, and has tremendous potential for rapid TES healing.

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Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An Injectable Black Phosphorus Hydrogel for Rapid Tooth Extraction Socket Healing

Guo,

Li,

Chen

et al. 2024

ACS Appl. Mater. Interfaces

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“…DL-3-n-butylphthalide (NBP), the first class I novel drug, which has been approved for the treatment of acute ischemia stroke in China since 2002 [9][10][11]. On account of the neuroprotective properties of NBP by eliminating free radicals, restoring mitochondrial function, reducing neuroinflammation and alleviating neuronal apoptosis, its therapeutic spectrum has expanded to various neurodegenerative diseases, such as Alzheimer disease (AD), Amyotrophic Laternal Sclerosis (ALS) and PD [12][13][14][15][16]. To date, many studies have clarified the benefit role of NBP in PD both in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Protective Effects of Dl-3-n-Butylphthalide against mpp + -Induced Toxicity via downregulating P53 pathway in N2A Cells

Zhao

Zhang

et al. 2023

Proteome Sci

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Background Dl-3-n-butylphthalide (NBP) is an important medial therapy for acute ischemic stroke in China. Recent studied have revealed that NBP not only rescued the loss of dopaminergic neurons in cellular and animal models of Parkinson's disease (PD), but also could improve motor symptoms in PD patients. However, the protective mechanism is not fully understood. P53 is a multifunctional protein implicated in numerous cellular processes, including apoptosis, DNA repair, mitochondrial functions, redox homeostasis, autophagy and protein aggregations. In PD, p53 integrated with various neurodegeneration-related signals inducing neuronal loss, indicating the suppression of P53 might be a promising target for PD treatment. Therefore, the purpose of the current study was to systemically screen new therapeutic targets of NBP in PD. Method In our study, we constructed mpp + induced N2A cells to investigate the benefit effect of NBP in PD. MTT assay was performed to evaluate the cell viability; TMT-based LC–MS/MS was applied to determine the different expressed proteins (DEPs) of NBP pretreatment; online bioinformatics databases such as DAVID, STRING, and KEGG was used to construe the proteomic data. After further analyzed and visualized the protein–protein interactions (PPI) by Cytoscape, DEPs were verified by western blot. Result A total of 5828 proteins were quantified in the comparative proteomics experiments and 417 proteins were considered as DEPs (fold change > 1.5 and p < 0.05). Among the 417 DEPs, 140 were upregulated and 277 were downregulated in mpp + -induced N2A cells with NBP pretreatment. KEGG pathway analysis indicated that lysosome, phagosome, apoptosis, endocytosis and ferroptosis are the mainly enriched pathways. By using MCL clustering in PPI analysis, 48 clusters were generated and the subsequent KEGG analysis of the top 3 clusters revealed that P53 signaling pathway was recognized as the dominant pathway for NBP treatment. Conclusion NBP significantly relived mpp + -induced cell toxicity. The neuroprotective role of NBP was implicated with P53 signaling pathway in some extent. These findings will reinforce the understanding of the mechanism of NBP in PD and identify novel therapeutic targets.

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“…After coronary recanalization in the infarcted area, arrhythmia, decline of cardiac function, and sudden cardiac death often occur clinically. The pathogenesis of MIRI is complex, involving oxidative damage ( 3 , 4 ), inflammatory response ( 5 , 6 ), calcium overload ( 7 , 8 ), and other processes. Many mechanisms of MIRI are unclear, especially the role of the immune microenvironment in MIRI remains to be further explored.…”

Section: Introductionmentioning

confidence: 99%

Identification of potential biomarkers associated with CD4+ T cell infiltration in myocardial ischemia-reperfusion injury using bioinformation analysis

Wang,

Kang,

et al. 2023

J Thorac Dis

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Background Myocardial ischemia-reperfusion injury (MIRI) is often part of clinical events such as cardiac arrest, resuscitation, and reperfusion after coronary artery occlusion. Recently, more and more studies have shown that the immune microenvironment is an integral part of ischemia-reperfusion injury (IRI), and CD4 + T-cell infiltration plays an important role, but there are no relevant molecular targets for clinical diagnosis and treatment. Methods The transcriptome data and matched group information were retrieved from the Gene Expression Omnibus (GEO) database. The ImmuCellAI-mouse (Immune Cell Abundance Identifier for mouse) algorithm was used to calculate each symbol’s CD4 + T cell infiltration score. The time period with the greatest change in the degree of CD4 + T cell infiltration [ischemia-reperfusion 6 hours (IR6h)-ischemia-reperfusion 24 hours (IR24h)] was selected for the next analysis. Weighted gene co-expression network analysis (WGCNA) and differential expression analysis were performed to screen out CD4 + T cell-related genes and from which the gene CLEC5A was screened for the highest correlation with CD4 + T cell infiltration. The potential regulatory mechanism of CD4 + T cells in MIRI was discussed through various enrichment analysis. Finally, we analyzed the expression and molecular function (MF) of CLEC5A and its related genes in MIRI. Results A total of 406 CD4 + T cell-related genes were obtained by intersecting the results of WGCNA and differential expression analysis. Functional enrichment analysis indicated that the CD4 + T cell-related genes were mainly involved in chemokine signaling pathway and cell cycle. By constructing a protein-protein interaction (PPI) network, a total of 12 hub genes were identified as candidate genes for further analysis. Through the correlation analysis between the 12 candidate genes found in the PPI network and CD4 + T cell infiltration fraction, we determined the core gene CLEC5A . Finally, a gene interaction network was constructed to decipher the biological functions of CLEC5A using GeneMANIA. Conclusions In this study, RNA sequencing (RNA-Seq) data at different time points after reperfusion were subjected to a series of bioinformatics methods such as PPI network, WGCNA module, etc., and CLEC5A , a pivotal gene associated with CD4 + T-cells, was found, which may serve as a new target for diagnosis or treatment.

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Dl-3-n-butylphthalide attenuates myocardial ischemia reperfusion injury by suppressing oxidative stress and regulating cardiac mitophagy via the PINK1/Parkin pathway in rats

Cited by 9 publications

References 36 publications

An Injectable Black Phosphorus Hydrogel for Rapid Tooth Extraction Socket Healing

An Injectable Black Phosphorus Hydrogel for Rapid Tooth Extraction Socket Healing

Proteomic Analysis of Protective Effects of Dl-3-n-Butylphthalide against mpp + -Induced Toxicity via downregulating P53 pathway in N2A Cells

Identification of potential biomarkers associated with CD4+ T cell infiltration in myocardial ischemia-reperfusion injury using bioinformation analysis

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