DJ-1 protects against undernutrition-induced atrophy through inhibition of the MAPK–ubiquitin ligase pathway in myoblasts

Kim, Junghwan; Won, Kyung‐Jong; Jung, Seung Hyo; Lee, Kang Pa; Shim, Sun Bo; Kim, Mee-Young; Kim, Ju-Hyun; Lee, Jeong-Uk

doi:10.1016/j.lfs.2015.09.016

Cited by 7 publications

(17 citation statements)

References 42 publications

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“…If future studies are performed on another posture, their results could be combined with those of the present research to inform physical therapy practice. Furthermore, further systematic and scientific studies in the fields of pain, rehabilitation and others are needed to confirm the effects of physical therapy 10 , 27 , 28 , 29 ) . In summary, we analyzed the body pressure-related sensory changes and compared them between the floor and mattress in a static supine position.…”

Section: Discussionmentioning

confidence: 99%

Differences in the body pressure-related sensory changes between the floor and mattress in a static supine position for physiotherapy research: a randomized controlled pilot trial

Lee

Kim

et al. 2016

J Phys Ther Sci

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[Purpose] This study was performed to investigate the difference in body pressure–related sensory changes between the floor and mattress in a static supine position for physiotherapy research. [Subjects and Methods] To analyze body pressure, the Body Pressure Measurement System was used. Body pressure sensors were attached to mattresses and the floor beneath the subjects. The level of pain was evaluated using pain score tools before the static supine position was adopted, at 1, 5, 10, and 15 min, and in total for specific body points. [Results] In analysis of digitized images, there was no significant difference observed between floor and mattress body pressure values at the start position. However, the head pressure intensity was significantly higher than that of the other body parts. In analysis of pain scores, all body part pain scores except those for both legs were significantly higher for the floor than for the mattress. Furthermore, the pain scores of the floor group were significantly increased at minute 1 compared with those of the mattress group. [Conclusion] These results suggest that properties that change in a time-dependent manner and postural changes need to be carefully considered when applying physical therapy.

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Section: Discussionmentioning

confidence: 99%

Differences in the body pressure-related sensory changes between the floor and mattress in a static supine position for physiotherapy research: a randomized controlled pilot trial

Lee

Kim

et al. 2016

J Phys Ther Sci

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“…Serum-free treatment increases mRNA and protein levels of atrogin-1 and MuRF-1 and decreases phosphorylation of Akt and FOXO in C2C12 and L6 myotubes (Kim et al, 2015;Sandri et al, 2004), indicating that the increase in atrogin-1 and MuRF-1 expression is controlled by the Akt/ FOXO pathway. However, a previous study in C2C12 myotubes showed that an AMPK activator increased atrogin-1 and MuRF-1 mRNA levels without affecting Akt phosphorylation (Egawa et al, 2014).…”

Section: Discussionmentioning

confidence: 95%

Myostatin Increases Smad2 Phosphorylation and Atrogin-1 Expression in Chick Embryonic Myotubes

2019

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Skeletal muscle mass is an important trait in poultry meat production. In mammals, myostatin, a negative regulator of skeletal muscle growth, activates Smad transcription factors and induces the expression of atrogin-1 by regulating the Akt/FOXO pathway. Although the amino acid sequence of chicken myostatin is known to be completely identical to its mammalian counterpart, previous studies in chicken skeletal muscles have implied that the physiological roles of chicken myostatin are different from those of mammals. Furthermore, it remains to be elucidated whether myostatin affects cellular signaling factors and atrogin-1 expression. In this study, using chick embryonic myotubes, we found that myostatin significantly increased the phosphorylation rate of Smad2 and mRNA levels of atrogin-1. No significant change was observed in the phosphorylation of Akt and FOXO1. These in vitro results suggest that the molecular mechanisms underlying myostatin-induced expression of atrogin-1 might be different between chickens and mammals.

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“…Male DJ-1 homozygous knockout (DJ-1 −/− , B6.Cg-Park7tm1shn/J, 25–30 g; n=6) and wild-type (DJ-1 +/+ ; n=6) mice with the same background were purchased from Jackson Laboratory 13 ) (Bar Harbor, ME, USA). To confirm DJ-1 gene depletion in mice, the distal tips of tails were obtained from DJ-1 −/− and DJ-1 +/+ mice.…”

Section: Methodsmentioning

confidence: 99%

“…Skeletal muscle atrophy has proven to be a significant orthopedic problem in the area of physical therapy 9 , 10 , 11 ) . Muscle atrophy has generally been reported that immobilization-induced atrophy, especially of physiotherapeutic area, decreased muscle volume via decrease of contractile proteins, resulting in muscle weakness and disorder of activities of daily living (ADL) 9 , 10 , 12 , 13 ) . In our previous study, it was demonstrated that cast-immobilization- and undernutrition-induced atrophy are correlated with each other via DJ-1 protein in skeletal muscle 13 ) .…”

Section: Introductionmentioning

confidence: 99%

“…Muscle atrophy has generally been reported that immobilization-induced atrophy, especially of physiotherapeutic area, decreased muscle volume via decrease of contractile proteins, resulting in muscle weakness and disorder of activities of daily living (ADL) 9 , 10 , 12 , 13 ) . In our previous study, it was demonstrated that cast-immobilization- and undernutrition-induced atrophy are correlated with each other via DJ-1 protein in skeletal muscle 13 ) . Furthermore, electrical properties such as rheobase and chronaxie are important factors in electrodiagnosis by physiotherapists for measurement of muscle degeneration such as total or partial muscle paralysis 14 , 15 ) .…”

Section: Introductionmentioning

confidence: 99%

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Differences in strength-duration curves of electrical diagnosis by physiotherapists between DJ-1 homozygous knockout and wild-type mice: a randomized controlled pilot trial

et al. 2016

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[Purpose] Strength-duration (SD) curves are used in electrical diagnosis by physiotherapists to confirm muscle degeneration. However, the usefulness of SD curves in comparing muscle degeneration in DJ-1 homozygous knockout (DJ-1−/−) and wild-type mice (DJ-1+/+) is not yet fully understood. The electrical properties of the gastrocnemius muscles of DJ-1−/− and DJ-1+/+ mice were compared in the current study. [Subjects and Methods] The electrode of an electrical stimulator was applied to the gastrocnemius muscle to measure the rheobase until the response of contractive muscle to electrical stimulation became visible in mice. [Results] The rheobase of DJ-1−/− mice showed a significant increase in a time-dependent manner, compared to that of DJ-1+/+ mice. [Conclusion] These results demonstrate that the DJ-1 protein may be implicated in the regulation of neuromuscular activity of gastrocnemius muscles of mice.

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DJ-1 protects against undernutrition-induced atrophy through inhibition of the MAPK–ubiquitin ligase pathway in myoblasts

Cited by 7 publications

References 42 publications

Differences in the body pressure-related sensory changes between the floor and mattress in a static supine position for physiotherapy research: a randomized controlled pilot trial

Differences in the body pressure-related sensory changes between the floor and mattress in a static supine position for physiotherapy research: a randomized controlled pilot trial

Myostatin Increases Smad2 Phosphorylation and Atrogin-1 Expression in Chick Embryonic Myotubes

Differences in strength-duration curves of electrical diagnosis by physiotherapists between DJ-1 homozygous knockout and wild-type mice: a randomized controlled pilot trial

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