Diyabetik sıçanların karaciğer ve böbrekleri üzerinde Caralluma tuberculata’nın hipoglisemik ve hipolipidemik etkisi ve güvenliği / Liquidambar orientalis Mill. reçine ekstraktlarının antioksidan, sitotoksik ve iNOS aktiviteleri

Abstract

Objective: The aim of this study is to investigate the in vitro cytotoxicity and iNOS (inducible nitric oxide synthase) inhibitory, and antioxidant activity in order to assess the traditional usage of Liquidambar orientalis Mill resin extract.Methods: Different solvent extracts of Liquidambar orientalis Mill resin were prepared. The cytotoxicity of extracts was determined using MTT (3-(4,5-dimethyl-2-thiazolyl)- 2,5-diphenyl-2H-tetrazoliumbromide) assay. HeLa (Human cervix adenocarcinoma), A-549 (Human alveolar adenocarcinoma), MCF-7 (Human breast adenocarcinoma), CaCo-2 (Human colon colorectal adenocarcinoma), mPANC96 (Human pancreas adenocarcinoma), PC-3 (Human prostate adenocarcinoma), U87MG (Human glioblastoma- astrocytoma) and as a normal cell line HEK293 (Human embryonic kidney cells) and Vero (African green monkey kidney epithelial cells) were used for testing cytotoxicity. RAW 264.7 (murine macrophage cell lines) was used to determine the inhibition levels of inducible nitric oxide synthase (iNOS). HL-60 (human acute myeloid leukemia) was used to determine antioxidant activity as DCF production per cent.Results: Hexane, dichloromethane, methanol and water extracts were prepared, and their iNOS inhibitory, cytotoxic and antioxidant activity were investigated. The estimated IC50 values of extracts varied from 6.68 to 48.90 μg/ ml after treatment with different doses of extracts for 48 h. Inhibition of the hexane, dichloromethane, methanol, and water extracts on LPS-induced NO production in RAW 264.7 macrophage were showed that the all extracts inhibited NO production in activated RAW 264.7 cells, except methanol extract. Hexane, dichloromethane and methanol extracts inhibited NO production with ICConclusion: This study is the first report showing the potential of Liquidambar orientalis Mill resin extracts for cytotoxicity, iNOS inhibition and the antioxidant activity as an alternative therapeutic approach for traditional uses. The results demonstrated that Liquidambar orientalis dichloromethane resin extracts showed strongest cytotoxic effect while all extracts except methanolic extracts exhibited moderate iNOS inhibition. All extracts other than hexane have a potent antioxidant effect in the cellular-based assay. In conclusion, further studies should focus on the purification of the active components of this extracts and to investigate the possible mode of action to obtain a better understanding of their potential use as cytotoxic, anti-inflammatory and antioxidant agents.

Evaluation of the Potential Therapeutic Properties of Liquidambar orientalis Oil

“…The reduction ability of gum was 0.91 mmol TE/g in the phosphomolybdenum assay. In a literature review, although we observed several studies on the antioxidant properties of L. orientalis extracts, [20][21][22] no data were obtained for gum. At this point, the presented results could make valuable contributions to the scientific pool of the Liquidambar genus.…”

Novel styrax liquidus loaded chitosan/polyvinyl alcohol cryogels with antioxidant and antimicrobial properties

“…Up to now, the chemical composition, antimicrobial, genotoxicity, mutagenicity, antioxidant potential, anti-ulcerogenic, antimutagenic and anticancer studies of styrax liquidus have been carried out. [18][19][20][21][22][23] In one of these studies, Nalbantsoy et al showed the cytotoxic, iNOS inhibition and antioxidant activity of Liquidambar orientalis Mill. Resin extracts prepared in different solvents.…”

“…The results showed that the resin exhibited diverse cytotoxic activity against different cell types including human cervix, alveolar, breast, colon colorectal, pancreas and prostate adenocarcinoma, human glioblastomaastrocytoma and as a normal cell line human embryonic kidney cells and African green monkey kidney epithelial cells. 23 However, to the best of our knowledge, there is no study about combining this natural plant product, which has unique properties and been used among the public for many years, with any engineered polymer based scaffold. The only study on combining styrax with a biomaterial was performed by Kılınç et al and they coated surgical silk sutures with styrax liquidus and determined their anti-adhesion capacity against oral pathogenic microorganisms.…”