Dixdc1 targets CyclinD1 and p21 via PI3K pathway activation to promote Schwann cell proliferation after sciatic nerve crush

Wu, Wenyan; Liu, Qingqing; Liu, Yuxi; Yu, Zhaohui; Wang, Youhua

doi:10.1016/j.bbrc.2016.08.058

Cited by 24 publications

(13 citation statements)

References 31 publications

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“…A number of cellular signaling pathways have been described to regulate the expression of cyclin D1 and p21. For instance, it has been recently reported that Dixdc1 (DIX domain containing 1) is capable of promoting Schwann cell proliferation by targeting cyclin D1 and p21 through PI3K activation [ 38 ]. Whether PI3K or any other cellular signaling pathways are involved in the ICA-mediated modulation of cyclin D1 and p21 regulation is worthy of further investigation.…”

Section: Discussionmentioning

confidence: 99%

Stimulatory effect of icariin on the proliferation of neural stem cells from rat hippocampus

Zhou

et al. 2018

BMC Complement Altern Med

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BackgroundIcariin (ICA), a major ingredient of Epimediumbrevicornum, has various pharmacological activities including central nervous system protective functions such as the improvement of learning and memory function in mice models of Alzheimer’s disease. It has been reported that ICA can promote regeneration of peripheral nerve and functional recovery. The purpose of this study was to investigate the potentiating effect of ICA on the proliferation of rat hippocampal neural stem cells, and explore the possible mechanism involved.MethodsPrimary neural stem cells were prepared from the hippocampus of newly born SD rats, and cells were cultured in special stem cell culture medium. Neural stem cells were confirmed by immunofluorescence detection of nestin, NSE and GFAP expression. The effect of ICA on the growth and proliferation of the neural stem cells was evaluated by 5-ethynyl-2-deoxyuridine (EdU) labeling of proliferating cells, and photomicrographic images of the cultured neural stem cells. Further, the mechanism of ICA-induced cell proliferation of neural stem cells was investigated by analyzing the gene and protein expression of cell cycle related genes cyclin D1 and p21.ResultsThe present study showed that icariin promotes the growth and proliferation of neural stem cells from rat hippocampus in a dose-dependent manner. Incubation of cells with icariin resulted in significant increase in the number of stem cell spheres as well as the increased incorporation of EdU when compared with cells exposed to control vehicle. In addition, it was found that icariin-induced effect on neural stem cells is associated with increased mRNA and protein expression of cell cycle genes cyclin D1 and p21.ConclusionsThis study evidently demonstrates the potentiating effect of ICA on neural stem cell growth and proliferation, which might be mediated through regulation of cell cycle gene and protein expression promoting cell cycle progression.Electronic supplementary materialThe online version of this article (10.1186/s12906-018-2095-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Stimulatory effect of icariin on the proliferation of neural stem cells from rat hippocampus

Zhou

et al. 2018

BMC Complement Altern Med

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“…PI3K/Akt cascade seems to provide trophic support for neurons, block apoptosis, facilitate signal transmission and mediate cell growth and differentiation in neurons. Also, it is reported that NGF is mediated by PI3K/Akt/mTOR pathway [281][282][283]. Ras-ERK pathway is a key promoter of neurite outgrowth and also has been found to enhance axonal survival [284].…”

Section: Pharmacological Agentsmentioning

confidence: 99%

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Pedrosa¹,

Caseiro²,

Santos³

et al. 2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

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Human adult peripheral nerve injuries are a high incidence clinical problem that greatly affects patients' quality of life. Although peripheral nervous system has intrinsic regenerative capacity, this occurs in an incomplete or poorly functional manner. When a nerve fiber loses its continuity with consequent damage of the basal lamina tubes, axon spontaneous regeneration is disorganized and mismatched. These phenomena translate in an inadequate nerve functional recovery and consequent musculoskeletal incapacity. Nerve grafts still remain the gold standard in peripheral injuries treatment. However, this approach contains its disadvantages such as the necessity of primary surgery to harvest the autografts, loss of a functional nerve, donor site morbidity and longer surgery procedures. Therefore, biomaterials and tissue engineering can provide efficient resources and alternatives to nerve injury repair not only by the development of biocompatible structures but also, introducing neurotrophic factors and cellular systems to stimulate optimum clinical outcome. In this chapter, a comprehensive state-of-the art picture of tissue-engineered nerve grafts scaffolds, their application in nerve regeneration along with latest advances in peripheral nerve repair and future perspectives will be discussed, including our own large experience in this field of knowledge.

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“…To verify the antitumor effects of ZNF259 in NSCLC, we screened the key signaling factors responsible for modulating their expression, such as FAK, AKT, and MAPK. 10 , 16 – 22 Results revealed that the levels of phosphorylated FAK and AKT were decreased upon ZNF259 overexpression. The decreased level of MMP2 was highly related to the inhibition of PI3K/Akt and ERK, both of which were downstream targets of FAK in breast cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…Cyclin D1, one of the key proteins of G1/S checkpoint, could promote cell cycle progression from G1 to S phase. 10 – 12 MMP2 could enhance the migration and invasion abilities of tumor cells. 13 , 14 Thus, we hypothesized that ZNF259 can function as a tumor suppressor by inhibiting the expression of cyclin D1 and MMP2.…”

Section: Discussionmentioning

confidence: 99%

ZNF259 inhibits non-small cell lung cancer cells proliferation and invasion by FAK-AKT signaling

Shan¹,

Cao²,

Wang³

et al. 2017

CMAR

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BackgroundZinc finger protein 259 (ZNF259) is known to play essential roles in embryonic development and cell cycle regulation. However, its expression pattern and clinicopathological relevance remain unclear.Materials and methodsA total of 114 lung cancer specimens were collected. The ZNF259 expression was measured between the lung cancer tissues and the adjacent normal lung tissues by immunohistochemical staining and Western blotting. Moreover, the correlation of ZNF259 expression with clinicopathological features was analyzed in 114 cases of lung cancer. Additionally, ZNF259 was depleted in the lung cancer cells in order to analyze its effect in the lung cancer.ResultsImmunohistochemical staining of 114 lung cancer specimens revealed significantly lower ZNF259 expression in lung cancer tissues than in adjacent normal lung tissues (53.5% vs 71.4%, P<0.001). In addition, ZNF259 downregulation was significantly associated with larger tumor size (P=0.001), advanced TNM stage (P=0.002), and positive lymph node metastasis (P=0.02). Western blotting of 20 paired lung cancer samples revealed lower ZNF259 protein levels in lung cancer tissues than in those of corresponding normal lung tissues (P=0.0032). Depletion of ZNF259 resulted in enhanced levels of p-FAK and p-AKT, CyclinD1, and MMP2, which in turn increased the proliferation and invasion of lung cancer cells. The effects of ZNF259 depletion were reversed by treatment with specific FAK or AKT inhibitors.ConclusionZNF259 depletion is correlated with the development of non-small cell lung cancer (NSCLC) and serves as a predictor of adverse clinical outcome in NSCLC patients. The inhibitory effect of ZNF259 on proliferation and invasion can be attributed to downregulation of CyclinD1 and MMP2 via inactivation of the FAK-AKT pathway.

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Dixdc1 targets CyclinD1 and p21 via PI3K pathway activation to promote Schwann cell proliferation after sciatic nerve crush

Cited by 24 publications

References 31 publications

Stimulatory effect of icariin on the proliferation of neural stem cells from rat hippocampus

Stimulatory effect of icariin on the proliferation of neural stem cells from rat hippocampus

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

ZNF259 inhibits non-small cell lung cancer cells proliferation and invasion by FAK-AKT signaling

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