DivIVA Phosphorylation Affects Its Dynamics and Cell Cycle in Radioresistant Deinococcus radiodurans

Chaudhary, Reema; Kota, Swathi; Misra, Hari S.

doi:10.1128/spectrum.03141-22

Cited by 4 publications

(3 citation statements)

References 63 publications

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“…Cells expressing phosphor-mimetic mutants of FtsZ behaved differently than cells expressing phosphor-ablative and wild type, indicating the arrest of FtsZ function upon phosphorylation by RqkA ( Chaudhary et al, 2023a ). Furthermore, DivIVA phosphorylation at the T19 site had altered its typical pattern of localization near the new septum site and completely distorted its ability to determine the plane of the next division that is normally perpendicular to the previous plane of cell division ( Chaudhary et al, 2023b ) The enhancement of DNA repair function with the concurrent arrest of cell division upon Ser/Thr phosphorylation by RqkA nearly mimics these processes upon DNA damage as observed in eukaryotes ( Sancar et al, 2004 ). Thus, a possibility of RqkA role in DNA damage response and cell cycle regulation in this bacterium, nearly similar to the Ser/Thr protein kinase (STPK) in eukaryotes (eSTPK) roles in eukaryotes, could be hypothesized.…”

Section: Deinococcus Radiodurans Encodes Putative Substrate ...mentioning

confidence: 99%

DNA damage response and cell cycle regulation in bacteria: a twist around the paradigm

Misra,

Rajpurohit

2024

Front. Microbiol.

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The co-protease activity in the RecA-ssDNA complex cleaves the autorepressor LexA, resulting in the derepression of a large number of genes under LexA control. This process is called the SOS response, and genes that are expressed in response to DNA damage are called SOS genes. The proteins encoded by the SOS genes are involved in both DNA repair and maintaining the functions of crucial cell division proteins (e.g., FtsZ) under check until the damaged DNA is presumably repaired. This mechanism of SOS response is the only known mechanism of DNA damage response and cell cycle regulation in bacteria. However, there are bacteria that do not obey this rule of DNA damage response and cell cycle regulation, yet they respond to DNA damage, repair it, and survive. That means such bacteria would have some alternate mechanism(s) of DNA damage response and cell cycle regulation beyond the canonical pathway of the SOS response. In this study, we present the perspectives that bacteria may have other mechanisms of DNA damage response and cell cycle regulation mediated by bacterial eukaryotic type Ser/Thr protein kinases as an alternate to the canonical SOS response and herewith elaborate on them with a well-studied example in the radioresistant bacterium Deinococcus radiodurans.

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Section: Deinococcus Radiodurans Encodes Putative Substrate ...mentioning

confidence: 99%

DNA damage response and cell cycle regulation in bacteria: a twist around the paradigm

Misra,

Rajpurohit

2024

Front. Microbiol.

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“…Deinococcus have also been reported to stop cell division in response to radiation stress; however, the exact mechanism of cell division arrest is not yet fully understood. Recent studies suggested that the phosphorylation of key cell division proteins such as FtsZ or DivIVA by the Serine/Threonine protein kinases (STPKs) (e.g., RqkA) could result in cell cycle arrest under radiation stress in D. radiodurans ( Chaudhary et al, 2023a ; Chaudhary et al, 2023b ). Importantly, both the Deinococcus strain (Ant6 and R1) contains large number of STPK but these proteins were not detected in the radioresistance strain K12 ( Supplementary Table S7 ).…”

Section: Interpretation Of Data Setmentioning

confidence: 99%

Complete genome sequence of Deinococcus rubellus Ant6 isolated from the fish muscle in the Antarctic Ocean

De Mandal,

Srinivasan,

Jeon

2023

Front. Bioeng. Biotechnol.

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“…antioxidants protection [31,38,, novel DNA repair proteins [64][65][66][67][68][69][70][71][72][73][74][75], cellular signaling constitute serine/ threonine kinase and cell division regulation [47,[76][77][78][79][80][81][82][83][84][85][86][87][88][89], and a compact nucleoid structure [64,88,90,91], all working together to ensure cell survival after exposure to extremely high doses of gamma rays, prolonged desiccation, and other DNA damaging agents. Additionally, D. radiodurans maintains its competency throughout the exponential growth phase, experiencing a decline in transforming frequency only during the stationary phase [92].…”

Section: Introductionmentioning

confidence: 99%

Surviving the Storm: Exploring the Role of Natural Transformation in Nutrition and DNA Repair of StressedDeinococcus radiodurans

Sharma,

Soni,

Gupta

et al. 2024

Preprint

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Deinococcus radiodurans, a natural transformation (NT) enabled bacterium renowned for its exceptional radiation resistance, employs unique DNA repair and oxidative stress mitigation mechanisms as a strategic response to DNA damage. This study excavate into the intricate roles of NT machinery in the stressedD. radiodurans, focusing on the genescomEA,comEC,endA,pilTanddprA, which are instrumental in the uptake and processing of extracellular DNA (eDNA). Our data reveals that NT not only supports the nutritional needs ofD. radioduransunder stress but also have roles in DNA repair. The study findings establish that NT-specific proteins (ComEA, ComEC, and EndA) might contribute to support the nutritional requirements in unstressed and heavily DNA-damaged cells while DprA contribute differently and in a context-dependent manner to navigating through the DNA damage storm. Thus, this dual functionality of NT-specific genes is proposed to be one of factor inD. radioduransremarkable ability to survive and thrive in environments characterized by high levels of DNA-damaging agents.Author Summary:Deinococcus radiodurans, a bacterium known for its extraordinary radiation resistance. This study explores the roles of natural transformation (NT) machinery in the radiation-resistant bacteriumDeinococcus radiodurans, focusing on the genescomEA,comEC,endA,pilT, anddprA. These genes are crucial for the uptake and processing of extracellular DNA (eDNA) and contribute to the bacterium nutritional needs and DNA repair under stress. The findings suggest that the NT-specific proteins ComEA, ComEC, and EndA may help meet the nutritional needs of unstressed and heavily DNA-damaged cells, whereas DprA plays a distinct role that varies depending on the context in aiding cells to cope with DNA damage. The functionality of NT genes is proposed to enhanceD. radioduranssurvival in environments with high levels of DNA-damaging agents.

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DivIVA Phosphorylation Affects Its Dynamics and Cell Cycle in Radioresistant Deinococcus radiodurans

Abstract: Deinococcus radiodurans , a radioresistant bacterium, lacks LexA/RecA-mediated DNA damage response and cell cycle regulation as known in other bacteria. However, it adjusts its transcriptome and proteome upon DNA damage.

Cited by 4 publications

References 63 publications

DNA damage response and cell cycle regulation in bacteria: a twist around the paradigm

DNA damage response and cell cycle regulation in bacteria: a twist around the paradigm

Complete genome sequence of Deinococcus rubellus Ant6 isolated from the fish muscle in the Antarctic Ocean

Surviving the Storm: Exploring the Role of Natural Transformation in Nutrition and DNA Repair of StressedDeinococcus radiodurans

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