Divided-aperture confocal Brillouin microscopy for simultaneous high-precision topographic and mechanical mapping

Wu, Hanxu; Zhao, Wenguang; Su, Yunhao; Qiu, Lirong; Wang, Yun; Ni, He

doi:10.1364/oe.405458

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…Due to the introduction of the divided-aperture, the optical signals between different layers are spatially separated, which can effectively reduce the interference of the noise signal of the defocused layer on the Raman spectrum at the focal point. In addition, the Brillouin scattered light and the reflected light are spatially separated, which effectively suppresses the interference of the reflected light and improves the system extinction ratio 32 (see Supporting Information for the extinction ratio test).…”

Section: Resultsmentioning

confidence: 99%

A high-precision multi-dimensional microspectroscopic technique for morphological and properties analysis of cancer cell

Qiu

et al. 2023

Light Sci Appl

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Raman and Brillouin scattering are sensitive approaches to detect chemical composition and mechanical elasticity pathology of cells in cancer development and their medical treatment researches. The application is, however, suffering from the lack of ability to synchronously acquire the scattering signals following three-dimensional (3D) cell morphology with reasonable spatial resolution and signal-to-noise ratio. Herein, we propose a divided-aperture laser differential confocal 3D Geometry-Raman-Brillouin microscopic detection technology, by which reflection, Raman, and Brillouin scattering signals are simultaneously in situ collected in real time with an axial focusing accuracy up to 1 nm, in the height range of 200 μm. The divided aperture improves the anti-noise capability of the system, and the noise influence depth of Raman detection reduces by 35.4%, and the Brillouin extinction ratio increases by 22 dB. A high-precision multichannel microspectroscopic system containing these functions is developed, which is utilized to study gastric cancer tissue. As a result, a 25% reduction of collagen concentration, 42% increase of DNA substances, 17% and 9% decrease in viscosity and elasticity are finely resolved from the 3D mappings. These findings indicate that our system can be a powerful tool to study cancer development new therapies at the sub-cell level.

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Section: Resultsmentioning

confidence: 99%

A high-precision multi-dimensional microspectroscopic technique for morphological and properties analysis of cancer cell

Qiu

et al. 2023

Light Sci Appl

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Dataset on capability of suppressing background noise and anti-tilting of divided-aperture differential confocal Raman microscopy system

Qiu

et al. 2021

Data in Brief

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Annular pupil confocal Brillouin–Raman microscopy for high spectral resolution multi-information mapping

Su,

Wu,

Qiu

et al. 2023

Nanophotonics

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Brillouin–Raman combined confocal spectroscopy is a novel and powerful technique for providing non-contact and direct readout of the micro-regional chemical and mechanical properties of a material, and thus used in a broad range of applications, including material characterization in manufacturing and biological imaging. However, the inadequate spectral and spatial resolution restricts the further development of combined spectral technology. In this paper, an annular pupil confocal Brillouin–Raman microscopy (APCBRM) scheme is proposed to achieve high-spectral-resolution Brillouin spectral detection and high-lateral-resolution Brillouin, Raman, and 3D topography mapping. The use of an annular pupil significantly suppresses the spectral broadening caused by a high-numerical-aperture objective lens and compresses the full width at half maximum of the Brillouin spectrum by 22.1 %, effectively improving the Brillouin spectral resolution. In addition, the size of the excitation spot is compressed, and the lateral resolutions in Brillouin and Raman spectroscopy increased to about 353.2 nm and 347.1 nm, respectively. Thus, high lateral resolution and Brillouin spectral resolution are achieved simultaneously. Furthermore, the high-precision confocal focusing system based on reflected light realizes real-time focusing during scanning and three-dimensional topography mapping. These results demonstrate that APCBRM has excellent potential for applications in the fields of novel materials, precision machining, and biomedicine.

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Divided-aperture confocal Brillouin microscopy for simultaneous high-precision topographic and mechanical mapping

Cited by 3 publications

References 36 publications

A high-precision multi-dimensional microspectroscopic technique for morphological and properties analysis of cancer cell

A high-precision multi-dimensional microspectroscopic technique for morphological and properties analysis of cancer cell

Dataset on capability of suppressing background noise and anti-tilting of divided-aperture differential confocal Raman microscopy system

Annular pupil confocal Brillouin–Raman microscopy for high spectral resolution multi-information mapping

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