Divide and differentiate

Ishidate, Takao; Elewa, Ahmed; Kim, Soyoung; Mello, Craig C.; Shirayama, Masaki

doi:10.4161/cc.28656

Cited by 34 publications

(20 citation statements)

References 49 publications

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“…We note that cell division, cell polarity and differentiation are all tightly coordinated during animal development (reviewed in refs 49, 50). In addition, our results indicate that PIG-1 influences proteins regulating myosin distribution and spindle positioning.…”

Section: Discussionmentioning

confidence: 99%

Comparative genetic, proteomic and phosphoproteomic analysis of C. elegans embryos with a focus on ham-1/STOX and pig-1/MELK in dopaminergic neuron development

Offenburger

Bensaddek

Murillo

et al. 2017

Sci Rep

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Asymmetric cell divisions are required for cellular diversity and defects can lead to altered daughter cell fates and numbers. In a genetic screen for C. elegans mutants with defects in dopaminergic head neuron specification or differentiation, we isolated a new allele of the transcription factor HAM-1 [HSN (Hermaphrodite-Specific Neurons) Abnormal Migration]. Loss of both HAM-1 and its target, the kinase PIG-1 [PAR-1(I)-like Gene], leads to abnormal dopaminergic head neuron numbers. We identified discrete genetic relationships between ham-1, pig-1 and apoptosis pathway genes in dopaminergic head neurons. We used an unbiased, quantitative mass spectrometry-based proteomics approach to characterise direct and indirect protein targets and pathways that mediate the effects of PIG-1 kinase loss in C. elegans embryos. Proteins showing changes in either abundance, or phosphorylation levels, between wild-type and pig-1 mutant embryos are predominantly connected with processes including cell cycle, asymmetric cell division, apoptosis and actomyosin-regulation. Several of these proteins play important roles in C. elegans development. Our data provide an in-depth characterisation of the C. elegans wild-type embryo proteome and phosphoproteome and can be explored via the Encyclopedia of Proteome Dynamics (EPD) – an open access, searchable online database.

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Section: Discussionmentioning

confidence: 99%

Comparative genetic, proteomic and phosphoproteomic analysis of C. elegans embryos with a focus on ham-1/STOX and pig-1/MELK in dopaminergic neuron development

Offenburger

Bensaddek

Murillo

et al. 2017

Sci Rep

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“…It is tempting to draw parallels between the fusome and asymmetrically distributed determinants in other systems. Asymmetric localization of proteins has been welldocumented (particularly in Drosophila neuroblasts and the early C. elegans embryo) (Lerit et al, 2013;Ishidate et al, 2014). RNA, organelles, and histones have also been recognized to be asymmetrically distributed in dividing cells (Ouellet and Barral, 2012;Noatynska et al, 2013;Smyth et al, 2015;Eritano et al, 2017;Kahney et al, 2017;Yamashita, 2018;Shlyakhtina et al, 2019).…”

Section: Open Questions and Concluding Remarksmentioning

confidence: 99%

Coordinating Proliferation, Polarity, and Cell Fate in the Drosophila Female Germline

Hinnant

Merkle

Ables

2020

Front. Cell Dev. Biol.

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Gametes are highly specialized cell types produced by a complex differentiation process. Production of viable oocytes requires a series of precise and coordinated molecular events. Early in their development, germ cells are an interconnected group of mitotically dividing cells. Key regulatory events lead to the specification of mature oocytes and initiate a switch to the meiotic cell cycle program. Though the chromosomal events of meiosis have been extensively studied, it is unclear how other aspects of oocyte specification are temporally coordinated. The fruit fly, Drosophila melanogaster, has long been at the forefront as a model system for genetics and cell biology research. The adult Drosophila ovary continuously produces germ cells throughout the organism's lifetime, and many of the cellular processes that occur to establish oocyte fate are conserved with mammalian gamete development. Here, we review recent discoveries from Drosophila that advance our understanding of how early germ cells balance mitotic exit with meiotic initiation. We discuss cell cycle control and establishment of cell polarity as major themes in oocyte specification. We also highlight a germline-specific organelle, the fusome, as integral to the coordination of cell division, cell polarity, and cell fate in ovarian germ cells. Finally, we discuss how the molecular controls of the cell cycle might be integrated with cell polarity and cell fate to maintain oocyte production.

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“…As key elements of the core cell cycle machinery, cyclins not only drive cell cycling as such, but integrate cellular proliferation with differentiation . Even though they are likely to be key regulatory components within the developmental programs that drive the hair cycle‐associated remodelling of human HF epithelium, cyclins have not before been comprehensively studied in the human HF, perhaps with the exception of cyclin D1 .…”

Section: Techniques and Read‐out Parametersmentioning

confidence: 99%

“…Even though they are likely to be key regulatory components within the developmental programs that drive the hair cycle‐associated remodelling of human HF epithelium, cyclins have not before been comprehensively studied in the human HF, perhaps with the exception of cyclin D1 . Cyclins bind with cyclin‐dependant kinases (CDKs) to engage in regulation of the cell cycle through their phosphorylation activity on specific proteins that are implicated in driving the transitions through G1 to M‐phase (,s16) (supplementary text S4; Table ; Fig. S6).…”

Section: Techniques and Read‐out Parametersmentioning

confidence: 99%

A primer for studying cell cycle dynamics of the human hair follicle

Purba

Brunken

Hawkshaw

et al. 2016

Experimental Dermatology

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Abstract:The cell cycle is of major importance to human hair follicle (HF) biology. Not only is continuously active cell cycling required to facilitate healthy hair growth in anagen VI HFs, but perturbations in the cell cycle are likely to be of significance in HF pathology (i.e. in scarring, non-scarring, chemotherapy-induced and androgenic alopecias). However, cell cycle dynamics of the human hair follicle (HF) are poorly understood in contrast to what is known in mouse. The current Methods Review aims at helping to close this gap by presenting a primer that introduces immunohistological/immunofluorescent techniques to study the cell cycle in the human HF. Moreover, this primer encourages the exploitation of the human HF as a powerful and clinically relevant tool to investigate mammalian cell cycle biology in situ. To achieve this, we describe methods to study markers of general 'proliferation' (nuclei count, Ki-67 expression), apoptosis (terminal deoxynucleotidyl transferase dUTP nick-end labelling, cleaved caspase 3), mitosis (phospho-histone H3, 'pS780'), DNA synthesis (5-ethynyl-2 0 -deoxyuridine) and cell cycle regulation (cyclins) in the human HF. In addition, we provide specific examples of dual immunolabelling for instructive cell cycle analyses and for investigating the cell cycle behaviour of specific HF keratinocyte subpopulations, such as keratin 15+ stem/ progenitor cells.

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Divide and differentiate

Cited by 34 publications

References 49 publications

Comparative genetic, proteomic and phosphoproteomic analysis of C. elegans embryos with a focus on ham-1/STOX and pig-1/MELK in dopaminergic neuron development

Comparative genetic, proteomic and phosphoproteomic analysis of C. elegans embryos with a focus on ham-1/STOX and pig-1/MELK in dopaminergic neuron development

Coordinating Proliferation, Polarity, and Cell Fate in the Drosophila Female Germline

A primer for studying cell cycle dynamics of the human hair follicle

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