Diversity, specificity and speciation in larval Diplostomidae (Platyhelminthes: Digenea) in the eyes of freshwater fish, as revealed by DNA barcodes

Locke, Sean A.; Al-Nasiri, Fatima Shihab; Caffara, Monica; Drago, Fabiana B.; Kalbe, Martin; Lapierre, Angela Rose; McLaughlin, John L.; Nie, Pin; Overstreet, Robin M.; Souza, Geza Thaís Rangel e; Takemoto, Ricardo Massato; Marcogliese, David J.

doi:10.1016/j.ijpara.2015.07.001

Cited by 99 publications

(137 citation statements)

References 63 publications

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“…Molecular methods have come a long way in allowing faster and more precise species identifications and the ability to make hypotheses about species relationships and evolution considering cryptic morphology. However, even with these methods, regional checklists of host–parasite relationships remain incomplete (Poulin, Besson, Morin, & Randhawa, ). One major issue is depauperate and biased databases, directly related to research and funding interests, expertise, and the natural evolution of improving methodologies over time.…”

Section: Introductionmentioning

confidence: 99%

A fine‐scale phylogenetic assessment of digenean trematodes in central Alberta reveals we have yet to uncover their total diversity

Gordy

Hanington

2019

Ecology and Evolution

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Despite over 100 years of digenean trematode parasite species descriptions, from a wide diversity of vertebrate and invertebrate host species, our ability to recognize the diversity of trematode species within a single lake remains an incredible challenge. The most challenging aspect is the identification of species from larval stages derived from intermediate hosts, due to the disjointed data of adult worm morphological descriptions, from which species are named, and links to corresponding molecular identifiers in depauperate databases. Cryptic species also play a significant role in the challenge of linking trematode larvae to adults, species identifications, and estimating diversity. Herein, we utilize a large, longitudinal dataset of snail first‐intermediate host infection data from lakes in Alberta, Canada, to infer trematode larval diversity using molecular phylogenetics and snail host associations. From our assessments, we uncover a diversity of 79 larval trematode species among just five snail host species. Only 14 species were identified to a previously described species, while the other 65 species are either cryptic or otherwise unrepresented by mitochondrial genes in GenBank. This study currently represents the largest and most diverse singular molecular survey of trematode larval fauna composed of over one thousand mitochondrial sequences. Surprisingly, rarefaction analyses indicate we have yet to capture the complete diversity of trematodes from our sampling area.

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Section: Introductionmentioning

confidence: 99%

A fine‐scale phylogenetic assessment of digenean trematodes in central Alberta reveals we have yet to uncover their total diversity

Gordy

Hanington

2019

Ecology and Evolution

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“…Discrepancies (e.g., Hysteromorpha in Hernández-Mena et al, 2017) are typically associated with poor nodal support. These genera are also consistently associated with Austrodiplostomum and Neodiplostomum (Blasco-Costa and Locke, 2017; Hernández-Mena et al, 2017; Locke et al, 2015; the latter sometimes misidentified as Fibricola , see Blasco-Costa and Locke, 2017), and the name Diplostomidae should be reserved for members of this group.…”

Section: Discussionmentioning

confidence: 99%

“…For example, the infection sites and encystment habits of metacercariae may be phylogenetically conserved. Diplostomid genera with metacercariae that reside unencysted in the eyes of second intermediate hosts consistently group together in molecular phylogenies (Blasco-Costa and Locke, 2017; Hernández-Mena et al, 2017) and in Diplostomum , habitats within the eye are conserved (Blasco-Costa et al, 2014) and may influence diversification (Locke et al, 2015). Some morphological characters are also more easily visualized in metacercariae.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the finding that Hysteromorpha is represented by a distinct species in North America, H. corti , is consistent with a general trend. With sequences now available from thousands of specimens and many valid and putative species of diplostomoids and clinostomids, intercontinental distributions supported by molecular data are exceedingly rare, and limited to distributions along the margins of a second continent (e.g., Austrodiplostomum compactum (= A. ostrowskiae ), Locke et al 2015). More commonly, a single species thought to cosmopolitan is revealed by DNA to be comprised of multiple geographically isolated species (e.g., Diplostomum spathaceum, D. baeri, Clinostomum complanatum , Caffara et al, 2011; Galazzo et al, 2002; Locke et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Most analyses of intra-diplostomoid relations are based on sequences of DNA from one or two loci totalling less than 2000 bp in length (e.g., Blasco-Costa and Locke, 2017, and references therein). This seems sufficient for specimen identification, discrimination of species, and species membership in genera (e.g., Chibwana et al 2013; Locke et al 2015; López-Hernández et al, 2018), but relationships among genera and families are less resolved. For example, there is inconsistency and often little support in the polarity among Alaria, Apharyngostrigea, and Cardiocephaloides in fig.…”

Section: Introductionmentioning

confidence: 99%

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Nuclear and mitochondrial phylogenomics of the Diplostomoidea and Diplostomida (Digenea, Platyhelminthes)¹

Caffara

et al. 2018

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phylogenetic studies, namely the possible placement of the Cyathocotylidae outside the 109 Diplostomoidea (Hernández-Mena et al., 2017), and the Diplostomidae as a basal lineage in the 110 order Plagiorchiida (Brabec et al., 2015). Both results originate from analysis of mtDNA, and we 111 obtained data to determine if these patterns were robust to additional taxon sampling. To decide 112 between what were likely to be conflicting topologies based on mtDNA and rDNA, we 113 employed phylogenomic analyses of ultra-conserved elements (UCEs). 114Although we set out to work on higher relationships among a small number of 115 representative specimens, we were aware that the diversity and identity of diplostomoid species 116 often differs from initial suspicions based on morphology (Blasco-Costa and Locke, 2017). We 117 therefore supported our identifications with morphological comparisons and analysis of 118 additional DNA sequences from closely related species, which led to several findings related to 119 the alpha taxonomy of the seven specimens of principal interest. 120 121 2. Materials and Methods 122 2.1 Specimen collection and identification 123

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Nonhost species reduce parasite infection in a focal host species within experimental fish communities

Ahn

Goater

2021

Ecology and Evolution

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Research concerning the biodiversity-disease relationship over the past 20 years has emphasized how changes in the diversity of hosts in a community, many of which are anthropogenically induced, influence the risk of disease transmission into a focal species of host (Halliday & Rohr, 2019;Johnson & Thieltges, 2010). Tests of the dilution effect hypothesis, which suggests that increased biodiversity (of both host and nonhost species) reduces infection risk within host communities, have been central to this research focus (Civitello et al., 2015;Keesing et al., 2006). The results of such tests have shown that increases in biodiversity can dilute the risk of transmission of infective stages of important human (e.g.,

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Diversity, specificity and speciation in larval Diplostomidae (Platyhelminthes: Digenea) in the eyes of freshwater fish, as revealed by DNA barcodes

Cited by 99 publications

References 63 publications

A fine‐scale phylogenetic assessment of digenean trematodes in central Alberta reveals we have yet to uncover their total diversity

A fine‐scale phylogenetic assessment of digenean trematodes in central Alberta reveals we have yet to uncover their total diversity

Nuclear and mitochondrial phylogenomics of the Diplostomoidea and Diplostomida (Digenea, Platyhelminthes)¹

Nonhost species reduce parasite infection in a focal host species within experimental fish communities

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Diversity, specificity and speciation in larval Diplostomidae (Platyhelminthes: Digenea) in the eyes of freshwater fish, as revealed by DNA barcodes

Cited by 99 publications

References 63 publications

A fine‐scale phylogenetic assessment of digenean trematodes in central Alberta reveals we have yet to uncover their total diversity

A fine‐scale phylogenetic assessment of digenean trematodes in central Alberta reveals we have yet to uncover their total diversity

Nuclear and mitochondrial phylogenomics of the Diplostomoidea and Diplostomida (Digenea, Platyhelminthes)1

Nonhost species reduce parasite infection in a focal host species within experimental fish communities

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Nuclear and mitochondrial phylogenomics of the Diplostomoidea and Diplostomida (Digenea, Platyhelminthes)¹