Diversity of Wolbachia in Natural Populations of Spider Mites (genus Tetranychus): Evidence for Complex Infection History and Disequilibrium Distribution

Zhang, Yankai; Zhang, Kaijun; Sun, Jing‐Tao; Yang, Xinming; Cheng, Ge; Hong, Xia

doi:10.1007/s00248-013-0198-z

Cited by 33 publications

(30 citation statements)

References 45 publications

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“…), the CI embryos took longer to hatching than the WU/WI controls. Altogether, these findings suggest that Wolbachia increases the probability of keeping Wolbachia ‐infected females in natural populations of T. urticae (Zhang et al, ).…”

Section: Discussionmentioning

confidence: 91%

Transcriptome of Tetranychus urticae embryos reveals insights into Wolbachia‐induced cytoplasmic incompatibility

et al. 2019

Insect Molecular Biology

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The endosymbiont Wolbachia is known for manipulating host reproduction in selfish ways. However, the molecular mechanisms have not yet been investigated in embryos. Here, we found that Wolbachia had no effect on the number of deposited eggs in Tetranychus urticae Koch (Acari: Tetranychidae) but caused two types of reproductive manipulation: killing uninfected female embryos via cytoplasmic incompatibility (CI) and increasing the hatching ratio of infected female embryos. RNA sequencing analyses showed that 145 genes were differentially expressed between Wolbachia‐infected (WI) and Wolbachia‐uninfected (WU) embryos. Wolbachia infection down‐regulated messenger RNA (mRNA) expression of glutathione S‐transferase that could buffer oxidative stress. In addition, 1613 and 294 genes were identified as CI‐specific up‐/down‐regulated genes. Compared to WU and WI embryos, embryos of CI cross strongly expressed genes involved in transcription, translation, tissue morphogenesis, DNA damage and mRNA surveillance. In contrast, most of the genes associated with energy production and metabolism were down‐regulated in the CI embryos compared to the WU and WI embryos, which provides some clues as to the cause of death of CI embryos. These results identify several genes that could be candidates for explaining Wolbachia‐induced CI. Our data form a basis to help elucidate the molecular consequences of CI in embryos.

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Section: Discussionmentioning

confidence: 91%

Transcriptome of Tetranychus urticae embryos reveals insights into Wolbachia‐induced cytoplasmic incompatibility

et al. 2019

Insect Molecular Biology

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“…The double infection of Wolbachia supergroups B and O in the same population indicates horizontal transmission of Wolbachia . Horizontal transmission of Wolbachia has often been speculated based on phylogenetic analysis (Werren et al 1995a; Sintupachee et al 2006; Stahlhut et al 2010; Schuler et al 2013; Zhang et al 2013). Wolbachia has also been reported from other whitefly genera such as Trialeurodes and some parasitoids (Raychoudhury et al 2009; Cass et al 2014), and this diversity of distribution may also hint horizontal transmission.…”

Section: Discussionmentioning

confidence: 99%

Diversity and evolution of the Wolbachia endosymbionts of Bemisia (Hemiptera: Aleyrodidae) whiteflies

Bing

Xia

Gui

et al. 2014

Ecology and Evolution

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Wolbachia is the most prevalent symbiont described in arthropods to date. Wolbachia can manipulate host reproduction, provide nutrition to insect hosts and protect insect hosts from pathogenic viruses. So far, 13 supergroups of Wolbachia have been identified. The whitefly Bemisia tabaci is a complex containing more than 28 morphologically indistinguishable cryptic species. Some cryptic species of this complex are invasive. In this study, we report a comprehensive survey of Wolbachia in B. tabaci and its relative B. afer from 1658 insects representing 54 populations across 13 provinces of China and one state of Australia. Based on the results of PCR or sequencing of the 16S rRNA gene, the overall rates of Wolbachia infection were 79.6% and 0.96% in the indigenous and invasive Bemisia whiteflies, respectively. We detected a new Wolbachia supergroup by sequencing five molecular marker genes including 16S rRNA, groEL, gltA, hcpA, and fbpA genes. Data showed that many protein-coding genes have limitations in detecting and classifying newly identified Wolbachia supergroups and thus raise a challenge to the known Wolbachia MLST standard analysis system. Besides, the other Wolbachia strains detected from whiteflies were clustered into supergroup B. Phylogenetic trees of whitefly mitochondrial cytochrome oxidase subunit I and Wolbachia multiple sequencing typing genes were not congruent. In addition, Wolbachia was also detected outside the special bacteriocytes in two cryptic species by fluorescence in situ hybridization, indicating the horizontal transmission of Wolbachia. Our results indicate that members of Wolbachia are far from well explored.

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“…Spider mite DNA was extracted as previously described52. The DNA quality was tested by amplifying a fragment of the cytochrome oxidase, subunit I ( COI ) gene of spider mites53.…”

Section: Methodsmentioning

confidence: 99%

Screening of spider mites (Acari: Tetranychidae) for reproductive endosymbionts reveals links between co-infection and evolutionary history

Zhang

Chen

Yang

et al. 2016

Sci Rep

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Reproductive endosymbionts have been shown to have wide-ranging effects on many aspects of their hosts’ biology. A first step to understanding how these endosymbionts interact with their hosts is to determine their incidences. Here, we screened for four reproductive endosymbionts (Wolbachia, Cardinium, Spiroplasma and Rickettsia) in 28 populations of spider mites (Acari: Tetranychidae) representing 12 species. Each of the four endosymbionts were identified in at least some of the tested specimens, and their infection patterns showed variations at the species-level and population-level, suggesting their distributions can be correlated with both the phylogeny and ecology of the hosts. Co-infections of unrelated bacteria, especially double infections of Wolbachia and Cardinium within the same individuals were common. Spiroplasma and Rickettsia infections were specific to particular host species, respectively. Further, the evolutionary histories of these endosymbionts were inferred by comparing the phylogenies of them and their hosts. These findings can help to clarify the interactions between endosymbionts and arthropods.

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Diversity of Wolbachia in Natural Populations of Spider Mites (genus Tetranychus): Evidence for Complex Infection History and Disequilibrium Distribution

Cited by 33 publications

References 45 publications

Transcriptome of Tetranychus urticae embryos reveals insights into Wolbachia‐induced cytoplasmic incompatibility

Transcriptome of Tetranychus urticae embryos reveals insights into Wolbachia‐induced cytoplasmic incompatibility

Diversity and evolution of the Wolbachia endosymbionts of Bemisia (Hemiptera: Aleyrodidae) whiteflies

Screening of spider mites (Acari: Tetranychidae) for reproductive endosymbionts reveals links between co-infection and evolutionary history

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