Diversity of the nucleic acid forms of circulating HBV in chronically infected patients and its impact on viral cycle

Abstract

and Roche. The public/private partnership is built in total transparency through a specific contract. The pharmaceutical companies are not involved in scientific decisions. The sponsor did not contribute to the study design and drafting of the study.The sponsor played no role in data collection, data analysis or data interpretation. The other funding sources played no role in study design, data collection, data analysis, data interpretation, or drafting the study.CF had full access to all data in the cohort study.

Biogenesis of serum HBV RNA and clinical phenomena of serum HBV RNA in chronic hepatitis B patients before and after receiving nucleos(t)ide analogues therapy

“…In most cases, the level of full-length pgRNA was reported to be significantly higher than spliced pgRNA. 10,18,31,35 However, compared to the 3.5 kb full-length pgRNA, extracellular HBV pgRNA exhibited shorter and varying lengths on Northern blot, most possibly due to the degradation of 3′ end of pgRNA. 12,13,15 Besides, pgRNA in CHB patients' sera was primarily polyadenylated near the conventional polyadenylation signal site.…”

“…While pgRNA predominates in extracellular HBV RNA, the question remains about which type of pgRNA accounts for the majority. In most cases, the level of full‐length pgRNA was reported to be significantly higher than spliced pgRNA 10,18,31,35 . However, compared to the 3.5 kb full‐length pgRNA, extracellular HBV pgRNA exhibited shorter and varying lengths on Northern blot, most possibly due to the degradation of 3′ end of pgRNA 12,13,15 .…”