Diversity of the culturable endophytic fungi producing L-asparaginase in arid Sinai, Egypt

Balbool, Bassem A.; Abdel-Azeem, Ahmed M.

doi:10.6092/issn.2531-7342/10063

Cited by 6 publications

(4 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…obtained from either plants, soil and marine algae were found to produce l- ASNase (Manasa and Nalini 2014 ). In fact, in a study examining 84 different fungal endophytes for their l- ASNase activity, an isolate of Fusarium proliferatum was observed to possess the best l- ASNase enzyme activity (Balbool and Abde L- Azeem 2020 ; Hatamzadeh et al 2020 ). Previous research investigated the L- ASNase produced by Fusarium solani along with the purification and characterization of the enzyme to obtain maximum yield (Uzma et al 2016 ; Zaki et al 2018 ; As-Suhbani and Bhosale 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Optimization and characterization of antileukemic l-asparaginase produced by Fusarium solani endophyte

Osama,

El-Sherei,

Al-Mahdy

et al. 2023

AMB Expr

View full text Add to dashboard Cite

Abstractl-asparaginase is an antileukemic enzyme that hydrolyzes l-asparagine into l-aspartic acid and ammonia, causing cell starvation and apoptosis in susceptible leukemic cell populations. Currently, l-asparaginase obtained from bacterial sources is constrained by several issues, including lesser productivity, stability, selectivity, and higher toxicity. The goal of this study is to provide fungal l-asparaginase with in-vitro effectiveness towards different human carcinomas. l-asparaginase from endophytic Fusarium solani (Gene Bank accession number MW209717) isolated from the roots of the medicinal plant Hedera helix L. was characterized and optimized experimentally for maximum l-asparaginase production in addition to evaluating its subsequent cytotoxicity towards acute monocytic leukemia and human skin fibroblast cell lines. The enzyme production was maximized using potato dextrose media (15.44 IU/ml/hr) at the 5th and 6th days of fermentation with incubation temperature 30 °C, 3% asparagine, 150–180 rpm agitation rate and a 250 ml flask. Enzyme characterization studies revealed that the enzyme maintained its thermal stability with temperatures up to 60 °C. However, its optimal activity was achieved at 35 °C. On measuring the enzymatic activity at various temperatures and different pH, maximum enzyme activity was recorded at 40 °C and pH 8 using 0.1 M asparagine concentration. Results also revealed promising cytotoxic activity against acute monocytic leukemia with IC50 = 3.66 µg/ml and low cytotoxicity against tested normal human skin fibroblast cell line which suggested that it might have selective toxicity, and consequently it could be used as a less toxic alternative to the current formulations.

show abstract

Section: Discussionmentioning

confidence: 99%

Optimization and characterization of antileukemic l-asparaginase produced by Fusarium solani endophyte

Osama,

El-Sherei,

Al-Mahdy

et al. 2023

AMB Expr

View full text Add to dashboard Cite

show abstract

“…Lasiodiplodia theobromae was obtained from the Suez Canal University Fungarium, Ismailia, Egypt (SCUF-TP2016). It was selected based on a continuing work from previous studies carried out by Balbool [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

“…Hence, researchers started to target fungi as a safe alternative eukaryotic source to produce asparaginase to decrease the associated allergic reactions [ 22 ]. Asparaginases were recorded from several fungal species such as Mucor hiemalis , Aspergillus niger , A. flavus , A. nidulans , A. terreus [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Insights into Asparaginase from Endophytic Fungus Lasiodiplodia theobromae: Purification, Characterization and Antileukemic Activity

Moubasher

Balbool

Helmy

et al. 2022

IJERPH

Self Cite

View full text Add to dashboard Cite

Endobiotic fungi are considered as a reservoir of numerous active metabolites. Asparaginase is used as an antileukemic drug specially to treat acute lymphoblastic leukaemia. The presented study aims to optimize the media conditions, purify, characterize, and test the antileukemic activity of the asparaginase induced from Lasiodiplodia theobromae. The culture medium was optimized using an experiment designed by The Taguchi model with an activity ranging from 10 to 175 IU/mL. Asparaginase was induced with an activity of 315 IU/mL. Asparaginase was purified with a specific activity of 468.03 U/mg and total activity of 84.4 IU/mL. The purified asparaginase showed an approximate size of 70 kDa. The purified asparaginase showed an optimum temperature of 37 °C and an optimum pH of 6. SDS reduced the activity of asparaginase to 0.65 U/mL while the used ionic surfactants enhanced the enzyme activity up to 151.92 IU/mL. The purified asparaginase showed a Km of 9.37 µM and Vmax of 127.00 µM/mL/min. The purified asparaginase showed an IC50 of 35.2 ± 0.7 IU/mL with leukemic M-NFS-60 cell lines and CC50 of 79.4 ± 1.9 IU/mL with the normal WI-38 cell line. The presented study suggests the use of endophytic fungi as a sustainable source for metabolites such as asparaginase, provides an opportunity to develop a facile, eco-friendly, cost-effective, and rapid synthesis of antileukemic drugs, which have the potential to be used as alternative and reliable sources for potent anticancer agents.

show abstract

Endophytic Fungi as a Source of New Pharmaceutical Biomolecules

et al. 2021

View full text Add to dashboard Cite

Diversity of the culturable endophytic fungi producing L-asparaginase in arid Sinai, Egypt

Cited by 6 publications

References 57 publications

Optimization and characterization of antileukemic l-asparaginase produced by Fusarium solani endophyte

Optimization and characterization of antileukemic l-asparaginase produced by Fusarium solani endophyte

Insights into Asparaginase from Endophytic Fungus Lasiodiplodia theobromae: Purification, Characterization and Antileukemic Activity

Endophytic Fungi as a Source of New Pharmaceutical Biomolecules

Contact Info

Product

Resources

About