Diversity of the cultivable gut bacterial communities associated with the fruit flies Bactrocera dorsalis and Bactrocera cucurbitae (Diptera: Tephritidae)

Gujjar, Nagalakshmi R.; Selvakumar, Govindan; Verghese, Abraham; Subramaniam, Sudhagar; More, Ravi Prabhakar

doi:10.1007/s12600-017-0604-z

Cited by 30 publications

(32 citation statements)

References 30 publications

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“…Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting and high throughput pyrosequencing studies of the 16S rRNA gene have highlighted the prevalence of microbial communities inhabiting the gut and reproductive organs of this insect, which play critical roles in host physiology and behavior (Andongma et al, 2015;Liu et al, 2016;Gujjar et al, 2017). Recent pyrosequencing analysis of the B. dorsalis microbiome revealed 172 operational taxonomic units (OTUs) assigned to six phyla (with Firmicutes as the most abundant in adult stages), five families, and 13 genera (Andongma et al, 2015;Liu et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Irradiation effect on the structure of bacterial communities associated with the oriental fruit fly, Bactrocera dorsalis

Stathopoulou

Asimakis

Khan

et al. 2019

Entomologia Exp Applicata

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The role of symbiotic microbes in insects, especially the beneficial character of this interaction for insects, has received much attention in recent years as it has been related to important aspects of the host insects' biology such as development, reproduction, survival, and fitness. Among insect hosts, tephritid fruit flies are well known to form beneficial associations with their symbionts. To control these destructive agricultural pests, environmentally friendly approaches, like the sterile insect technique as a component of integrated pest management strategies, remain most effective. In this study, changes in the bacterial profile of mass‐reared oriental fruit flies, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), were examined in both larval and adult stages and also after irradiation by employing a 16S rRNA gene‐based Illumina sequencing approach. Proteobacteria was the prevalent bacterial phylum in non‐irradiated adults and larvae. Alphaproteobacteria was the most abundant class in larvae but almost absent in adults, which was dominated by Gammaproteobacteria. Firmicutes were present in both developmental stages but at lower relative abundance. At genus level, Acetobacter prevailed in the larval stage and members of the Enterobacteriaceae family in adults. Irradiated samples exhibited higher diversity and richness indices compared to the non‐irradiated oriental fruit flies, whereas no significant changes were observed between the two developmental stages of the non‐irradiated samples. Lactobacillus, members of the Orbacecae family, and Morganella were detected but to a lesser degree upon irradiation, whereas the relative abundance of Lactococcus and Orbus increased. The bacterial profile of larvae appeared to be different compared to that of adult B. dorsalis flies. The subsequent application of irradiation at the pupal stage led to the development of different microbiota between treated and untreated samples, affecting diversity and operational taxonomic unit composition. Irradiated samples of oriental fruit flies were characterized by higher species diversity and richness.

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Section: Introductionmentioning

confidence: 99%

“…Other bacterial OTUs like Listeria (Listeriaceae), Pseudomonas aeruginosa (Schr€ oter) Migula (Pseudomonadaceae), Enterococcus sp., (Enterococcaceae), and Lactobacillus sp. (Streptococcaceae) were also identified from B. dorsalis but they were less abundant (Gujjar et al, 2017;Khaeso et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Irradiation effect on the structure of bacterial communities associated with the oriental fruit fly, Bactrocera dorsalis

Stathopoulou

Asimakis

Khan

et al. 2019

Entomologia Exp Applicata

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“…The gut microbiome of B. dorsalis derived from different populations in Kenya was found to consist of members that have been reported among the gut microbiota of B. dorsalis in other parts of the world [1,[22][23][24][25][26][27][28][29][30][31]. We found that B. dorsalis can be re-inoculated with bacterial isolates at the embryo stage and that the effects of such isolates on host development and susceptibility to pathogens can subsequently be evaluated.…”

Section: Discussionmentioning

confidence: 72%

“…A number of studies have investigated the diversity and described the gut microbiome structure of B. dorsalis [1,[22][23][24][25][26][27][28][29][30]. The speci c roles of certain bacterial isolates from B. dorsalis have also been reported.…”

Section: Introductionmentioning

confidence: 99%

Influence of Bactrocera Dorsalis Larval Gut Bacteria on its Susceptibility to the Entomopathogenic Fungus, Metarhizium Anisopliae

Gichuhi¹,

Khamis²,

Berg³

et al. 2020

Preprint

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Symbiotic interactions between insects and bacteria have been associated with a vast variety of physiological, ecological and evolutionary consequences for the host. A wide range of bacterial communities have been found in association with the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), an important pest of cultivated fruit in most regions of the world. We evaluated the diversity of gut bacteria in B. dorsalis specimens from several populations in Kenya and investigated the roles of individual bacterial isolates in the development of axenic B. dorsalis fly lines and their responses to the entomopathogenic fungus, Metarhizium anisopliae. We utilized a high throughput sequencing approach of the bacterial 16S rRNA gene to evaluate microbiomes, coupled with culture and mono-association for the assay. The shortest embryonic development period was recorded in flies with an intact gut microbiome while the longest period was recorded in germ-free fly lines. Similarly, larval development was shortest in flies with an intact gut microbiome. Adult B. dorsalis flies emerging from embryos inoculated with a strain of Lactococcus lactis had decreased survival when challenged with a standard dosage of M. anisopliae ICIPE69 conidia. However, there were no differences in survival between the germ-free lines and flies with an intact microbiome. These findings contribute to the selection of probiotics used in artificial diets for B. dorsalis rearing and the development of improved integrated pest management strategies based on entomopathogenic fungi.

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“…The role of bacteria as a food source for adult fruit flies and how they affect their behaviour and fitness have been studied extensively by Drew et al, 1983;Drew and Lloyd, 1987, 1989, 1990Drew, 1987 andby Fitt andO'Brien (1985), who reported that some bacteria found on ripening fruits also exist in the digestive tract of flies and that females transmit these bacteria to their offspring during oviposition. Gujjar et al (2017) attempted to decipher the gender specificity of gut bacterial communities of two major fruit fly species of India and based on molecular identification, B. dorsalis females were found to predominantly harbor the bacterial species Enterobacter cloacae, E. asburiae and Citrobacter freundii, while B. dorsalis males were found to harbor Providencia rettgerii, Klebsiella oxytoca, Enterococcus faecalis and Pseudomonas aeruginosa. The cultivable diversity from females of Z. cucurbitae comprised mainly of Morganella morganii and Bacillus pumilis while Z. cucurbitae males were predominantly colonized by aerobic endospore formers, viz.…”

Section: Bacterial Associationsmentioning

confidence: 99%

An Overview of Indian Dacine Fruit Flies (Diptera: Tephritidae: Dacinae: Dacini)

Vasudha¹,

Ahmad²,

Agarwal³

2019

IJBSM

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Fruit flies belonging to tribe Dacini are pertinent pests of horticultural crops in Southeast Asia, Sub-Saharan Africa and Australasian Oceanian region. They pose enormous threats to fruit and vegetable production throughout the world, causing both quantitative and qualitative losses. Ninety two species of dacine fruit flies (28 endemic, 30.43% endemism) belonging to three genera, viz. Bactrocera Macquart (51 species, 17 endemic, 33.33% endemism), Dacus Fabricius (10 species, 3 endemic, 30.0% endemism), and Zeugodacus Hendel (31 species, 8 endemic, 25.80% endemism), and 19 subgenera have been listed from India. Of these, nine endemic species, viz. Bactrocera (Bactrocera) andamanensis, B. (B.) blairiae, B. (B.) curtivitta, B. (B.) patula, B. (B.) ranganathi, B. (Calodacus) harrietensis, Dacus (Mellesis) insulosus, Zeugodacus (Zeugodacus) fuscoalatus, Z. (Z.) havelockiae are known only from Andaman & Nicobar Islands. The current understanding of the morphology of the tribe, together with data on their biology, habitat associations, and distribution combine presents a picture of the phylogenetic relationships within the group. Besides, the knowledge of biology and behaviour of dacine species suggests that there is a close relationship between the fly species and their host plants which involves adult's feeding, host recognition, courtship and mating, oviposition, larval feeding and bacteria associations. In the present paper notes on subgeneric classification of Dacini, their distribution, endemism, relative occurrence of different taxa, phylogeny, host associations, pest status, male lures and bacterial associations have been added.

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Diversity of the cultivable gut bacterial communities associated with the fruit flies Bactrocera dorsalis and Bactrocera cucurbitae (Diptera: Tephritidae)

Cited by 30 publications

References 30 publications

Irradiation effect on the structure of bacterial communities associated with the oriental fruit fly, Bactrocera dorsalis

Irradiation effect on the structure of bacterial communities associated with the oriental fruit fly, Bactrocera dorsalis

Influence of Bactrocera Dorsalis Larval Gut Bacteria on its Susceptibility to the Entomopathogenic Fungus, Metarhizium Anisopliae

An Overview of Indian Dacine Fruit Flies (Diptera: Tephritidae: Dacinae: Dacini)

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