Diversity of the Bacterial Microbiota of Anopheles Mosquitoes from Binh Phuoc Province, Vietnam

Ngo, Chung Thuy; Romano-Bertrand, Sara; Manguin, Sylvie; Jumas‐Bilak, Estelle

doi:10.3389/fmicb.2016.02095

Cited by 35 publications

(38 citation statements)

References 62 publications

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“…This trend was replicated in the other study sites. Thus, it is clear from these results that variation in microbiota between mosquito species may occur in some sampling locations but not in others, which is consistent with previous findings [ 32 – 34 , 55 ].…”

Section: Discussionsupporting

confidence: 92%

Mosquito microbiota cluster by host sampling location

et al. 2018

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BackgroundMicrobial communities that inhabit the mosquito body play an import role in host biology and may have potential for mosquito control. However, the forces that shape these microbial communities are poorly understood.MethodsTo gain a better understanding of how host location influences the composition and diversity of mosquito microbiota, we performed a survey of microbial communities in mosquito samples collected from six USA states using HiSeq sequencing of the 16S rRNA gene.ResultsA total of 284 bacterial operational taxonomic units (OTUs) belonging to 14 phyla were detected in nine mosquito species, with Proteobacteria, Firmicutes and Actinobacteria accounting for 95% of total sequences. OTU richness varied markedly within and between mosquito species. The microbial composition and diversity was heavily influenced by the site of mosquito collection, suggesting that host location plays an important role in shaping the mosquito microbiota.ConclusionsVariation in microbial composition and diversity between mosquitoes from different locations may have important implications on vector competence and transmission dynamics of mosquito-borne pathogens. Future studies should investigate the environmental factors responsible for these variations and the role of key bacteria characterized in this study on mosquito biology and their potential application in symbiotic control of mosquito-borne diseases.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-3036-9) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 92%

Mosquito microbiota cluster by host sampling location

et al. 2018

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“…Our ndings are consistent with previous studies that showed that collection site was a major contributor to the microbial diversity of eld-caught Anopheles mosquitoes [26,[49][50][51]. For example, Muturi et al…”

Section: Discussionsupporting

confidence: 93%

Relative Contributions of Genetic and Non-Genetic Factors on Mosquito Microbiota

Bogale

Cannon

Keita³

et al. 2020

Preprint

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Background The commensal microbiota of mosquitoes impacts their development, immunity, and competency, and could provide a target for alternative entomological control approaches. However, despite the importance of the mosquito/microbiota interactions, little is known about the relative contribution of genetic and non-genetic factors in shaping the bacterial communities of mosquitoes. Methods We used a high-throughput sequencing based assay to characterize the bacterial composition and diversity of 665 individual field-caught mosquitoes, as well as their species, genotype at an insecticide resistance locus, blood meal composition, and the eukaryotic parasites and viruses they carry. We then used these data to rigorously estimate the individual effect of each parameter on the bacterial diversity as well as their relative contribution to the microbial composition. Results Overall, multivariate analyses did not reveal any significant contribution of the mosquito species, insecticide resistance or blood meal to the bacterial composition of the mosquitoes surveyed. Infection with parasites and viruses only contributed very marginally and the main driver of the bacterial diversity was the location where each mosquito was collected which explained roughly 20% of the variance observed. Conclusions This analysis shows that, when confounding factors are taken into account, the sites where the mosquitoes are collected is the main driver of the bacterial diversity of wild-caught mosquitoes, although further studies will be needed to determine how the specific components of the local environment affecting the bacterial composition.

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“…bacteria, which correspond to the most abundant taxa associated with those populations [ 18 ]. In Vietnam, Acinetobacter was found to be the sole core microbiota constituent of 11 Anopheles species [ 30 , 31 ]. Indigenous and invasive populations of Ae.…”

Section: Mosquito Core- and Pan-microbiotamentioning

confidence: 99%

The mosquito holobiont: fresh insight into mosquito-microbiota interactions

et al. 2018

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The holobiont concept was first developed for coral ecosystems but has been extended to multiple organisms, including plants and other animals. Studies on insect-associated microbial communities have produced strong evidence that symbiotic bacteria play a major role in host biology. However, the understanding of these symbiotic relationships has mainly been limited to phytophagous insects, while the role of host-associated microbiota in haematophagous insect vectors remains largely unexplored. Mosquitoes are a major global public health concern, with a concomitant increase in people at risk of infection. The global emergence and re-emergence of mosquito-borne diseases has led many researchers to study both the mosquito host and its associated microbiota. Although most of these studies have been descriptive, they have led to a broad description of the bacterial communities hosted by mosquito populations. This review describes key advances and progress in the field of the mosquito microbiota research while also encompassing other microbes and the environmental factors driving their composition and diversity. The discussion includes recent findings on the microbiota functional roles and underlines their interactions with the host biology and pathogen transmission. Insight into the ecology of multipartite interactions, we consider that conferring the term holobiont to the mosquito and its microbiota is useful to get a comprehensive understanding of the vector pathosystem functioning so as to be able to develop innovative and efficient novel vector control strategies.Electronic supplementary materialThe online version of this article (10.1186/s40168-018-0435-2) contains supplementary material, which is available to authorized users.

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Diversity of the Bacterial Microbiota of Anopheles Mosquitoes from Binh Phuoc Province, Vietnam

Cited by 35 publications

References 62 publications

Mosquito microbiota cluster by host sampling location

Mosquito microbiota cluster by host sampling location

Relative Contributions of Genetic and Non-Genetic Factors on Mosquito Microbiota

The mosquito holobiont: fresh insight into mosquito-microbiota interactions

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