Diversity of Survival Patterns among Escherichia coli O157:H7 Genotypes Subjected to Food-Related Stress Conditions

Elhadidy, Mohamed; Álvarez‐Ordóñez, Avelino

doi:10.3389/fmicb.2016.00322

Cited by 13 publications

(7 citation statements)

References 34 publications

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“…E. coli O157:H7 is one of the most virulent pathogens responsible for foodborne disease globally. Cattle are the main host for E. coli O157:H7 and outbreaks are frequently attributed to the ingestion of contaminated milk and dairy products of cattle origin [ 14 ]. In 1999, a large-scale E. coli O157:H7 outbreak in China had resulted in 195 hospital admissions due to hemolytic uremic syndrome and 177 deaths [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

Food Safety Knowledge, Attitudes and Behavior among Dairy Plant Workers in Beijing, Northern China

Chen

Chen³

et al. 2018

IJERPH

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The safety of milk and dairy products has always been one of the focuses of consumers, the food industry and regulatory agencies. The purpose of this study was to gain insight into the food safety knowledge, attitudes and behavior of dairy plant workers. A cross-sectional survey was performed between May and August 2015 in three dairy plants in Beijing, northern China. A total of 194 dairy plant workers were interviewed to collect information on food safety knowledge, attitudes and self-reported behavior. The 194 dairy plant workers interviewed showed a sufficient level of knowledge (mean score 34 on a scale from 0–58), perfect attitudes (mean score 17 on a scale from 0–18), and perfect behavior (mean score 38 on a scale from 8–40). Only 39% of workers correctly determined specific pathogens or diseases that could be conveyed through milk and dairy products. 24% of workers knew the correct method of washing hands. A significant positive association was observed between attitudes and knowledge (p < 0.001) as well as behavior (p < 0.01). Education level was positively and significantly associated with food safety knowledge, attitudes, and behavior (p < 0.05). Workers in dairy enterprises in northern China have relatively low levels of knowledge, yet satisfactory attitudes and behavior. The knowledge of microbial food hazards and hand hygiene remains an issue that needs to be emphasized in future training programs. Education level is a determinant of attitudes and behavior with regard to the proper handling of milk and dairy products.

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Section: Discussionmentioning

confidence: 99%

Food Safety Knowledge, Attitudes and Behavior among Dairy Plant Workers in Beijing, Northern China

Chen

Chen³

et al. 2018

IJERPH

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“…All strains isolated belonged to LSPA‐6 lineage I/II. Interestingly, Elhadidy and Álvarez‐Ordóñez () reported that lineage I/II strains were significantly more resistant to acid, cold and starvation stress than lineage II strains, and this fact could explain this persistence in the environment.…”

Section: Resultsmentioning

confidence: 99%

Virulence profile ofEscherichia coliO157 strains isolated from surface water in cattle breeding areas

Tanaro

Pianciola

D'Astek

et al. 2018

Lett Appl Microbiol

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The challenge for the growing global demand for food is to find sustained production strategies without collateral effects. Intensive livestock operations generate large volumes of manure that can contaminate a finite resource, the water. This study shows how water contaminated by confined feeding operations can transport dangerous pathogens and warns to pay more attention to control and sanitation systems to prevent this type of pollution.

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“…Pathogenicity in humans is inexorably linked to the Stx litres produced by individual STEC strains [15, 16]. Hypervirulent clones, as manifested by increased Stx 2a -litres [17–24], have been associated with STEC subpopulations through phylogenetic, epidemiological, and phenotypic linkage [4, 5, 11, 19, 20, 25–49]. The specific factors responsible for elevated Stx-production in hypervirulent STEC strains are unknown but presumably modulated by a number of biotic and abiotic triggers [6, 8, 50–54].…”

Section: Introductionmentioning

confidence: 99%

Pathogenomes and variations in Shiga toxin production among geographically distinct clones of Escherichia coli O113:H21

et al. 2022

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Infections with globally disseminated Shiga toxin-producing Escherichia coli (STEC) of the O113:H21 serotype can progress to severe clinical complications, such as hemolytic uremic syndrome (HUS). Two phylogeographically distinct clonal complexes have been established by multi locus sequence typing (MLST). Infections with ST-820 isolates circulating exclusively in Australia have caused severe human disease, such as HUS. Conversely, ST-223 isolates prevalent in the US and outside Australia seem to rarely cause severe human disease but are frequent contaminants. Following a genomic epidemiology approach, we wanted to gain insights into the underlying cause for this disparity. We examined the plasticity in the genome make-up and Shiga toxin production in a collection of 20 ST-820 and ST-223 strains isolated from produce, the bovine reservoir, and clinical cases. STEC are notorious for assembly into fragmented draft sequences when using short-read sequencing technologies due to the extensive and partly homologous phage complement. The application of long-read technology (LRT) sequencing yielded closed reference chromosomes and plasmids for two representative ST-820 and ST-223 strains. The established high-resolution framework, based on whole genome alignments, single nucleotide polymorphism (SNP)-typing and MLST, includes the chromosomes and plasmids of other publicly available O113:H21 sequences and allowed us to refine the phylogeographical boundaries of ST-820 and ST-223 complex isolates and to further identify a historic non-shigatoxigenic strain from Mexico as a quasi-intermediate. Plasmid comparison revealed strong correlations between the strains’ featured pO113 plasmid genotypes and chromosomally inferred ST, which suggests coevolution of the chromosome and virulence plasmids. Our pathogenicity assessment revealed statistically significant differences in the Stx2a-production capabilities of ST-820 as compared to ST-223 strains under RecA-induced Stx phage mobilization, a condition that mimics Stx-phage induction. These observations suggest that ST-820 strains may confer an increased pathogenic potential in line with the strain-associated epidemiological metadata. Still, some of the tested ST-223 cultures sourced from contaminated produce or the bovine reservoir also produced Stx at levels comparable to those of ST-820 isolates, which calls for awareness and for continued surveillance of this lineage.

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Diversity of Survival Patterns among Escherichia coli O157:H7 Genotypes Subjected to Food-Related Stress Conditions

Cited by 13 publications

References 34 publications

Food Safety Knowledge, Attitudes and Behavior among Dairy Plant Workers in Beijing, Northern China

Food Safety Knowledge, Attitudes and Behavior among Dairy Plant Workers in Beijing, Northern China

Virulence profile ofEscherichia coliO157 strains isolated from surface water in cattle breeding areas

Pathogenomes and variations in Shiga toxin production among geographically distinct clones of Escherichia coli O113:H21

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