Diversity of   strains isolated from the rhizosphere of maize planted in Cerrado soil

Weid, Irene von der; Paiva, Edílson; Nóbrega, Alberto; Elsas, Jan Dirk van; Seldin, Lucy

doi:10.1016/s0923-2508(00)00160-1

Cited by 89 publications

(53 citation statements)

References 54 publications

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“…Identifications of the products amplified with the first primer set (V2-V3) showed the dominance of P. polymyxa sequences. P. polymyxa is known to be a colonizer of maize rhizospheres (32,39). The sequences detected in these profiles were all different from each other 14) and a bacterial cell consortium extracted from a maize rhizosphere (lane 15).…”

Section: Resultsmentioning

confidence: 96%

Effect of Primers Hybridizing to Different Evolutionarily Conserved Regions of the Small-Subunit rRNA Gene in PCR-Based Microbial Community Analyses and Genetic Profiling

Schmalenberger

Schwieger

Tebbe

2001

Appl Environ Microbiol

247

177

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Genetic profiling techniques of microbial communities based on PCR-amplified signature genes, such as denaturing gradient gel electrophoresis or single-strand-conformation polymorphism (SSCP) analysis, are normally done with PCR products of less than 500-bp. The most common target for diversity analysis, the small-subunit rRNA genes, however, are larger, and thus, only partial sequences can be analyzed. Here, we compared the results obtained by PCR targeting different variable (V) regions (V2 and V3, V4 and V5, and V6 to V8) of the bacterial 16S rRNA gene with primers hybridizing to evolutionarily conserved flanking regions. SSCP analysis of single-stranded PCR products generated from 13 different bacterial species showed fewer bands with products containing V4-V5 (average, 1.7 bands per organism) than with V2-V3 (2.2 bands) and V6-V8 (2.3 bands). We found that the additional bands (>1 per organism) were caused by intraspecies operon heterogeneities or by more than one conformation of the same sequence. Community profiles, generated by PCR-SSCP from bacterial-cell consortia extracted from rhizospheres of field-grown maize (Zea mays), were analyzed by cloning and sequencing of the dominant bands. A total of 48 sequences could be attributed to 34 different strains from 10 taxonomical groups. Independent of the primer pairs, we found proteobacteria (␣, ␤, and ␥ subgroups) and members of the genus Paenibacillus (low G؉C gram-positive) to be the dominant organisms. Other groups, however, were only detected with single primer pairs. This study gives an example of how much the selection of different variable regions combined with different specificities of the flanking "universal" primers can affect a PCR-based microbial community analysis.

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Section: Resultsmentioning

confidence: 96%

Effect of Primers Hybridizing to Different Evolutionarily Conserved Regions of the Small-Subunit rRNA Gene in PCR-Based Microbial Community Analyses and Genetic Profiling

Schmalenberger

Schwieger

Tebbe

2001

Appl Environ Microbiol

247

177

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“…The close relationship between P. peoriae and P. polymyxa and P. brasilensis, both species able to produce antimicrobial substances (Kajimura and Kaneda 1997;von der Weid et al 2000von der Weid et al , 2002 was demonstrated genetically and phenotypically. For this reason, we decided to investigate whether strains of P. peoriae also have antimicrobial properties.…”

Section: Discussionmentioning

confidence: 97%

Antimicrobial activity of Paenibacillus peoriae strain NRRL BD-62 against a broad spectrum of phytopathogenic bacteria and fungi

Weid¹,

Alviano²,

Santos³

et al. 2003

J Appl Microbiol

107

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Aims: To investigate the potential antagonistic activity of Paenibacillus peoriae strain NRRL BD-62 against phytopathogenic micro-organisms and to determine the physiological and biochemical characteristics of the antimicrobial compound produced by this strain. Methods and Results: Strain NRRL BD-62 showed a broad inhibition spectrum with activity against various phytopathogenic bacteria and fungi. Physico-chemical characterization of the antimicrobial activity showed that it was stable during heat treatment and was retained even after autoclave at 121°C for 10 min. The compound was also stable after the treatment with organic solvents, hydrolytic enzymes and its activity was preserved at a wide range of pH. The partial purification carried out by Sephadex G25 gel filtration showed two profiles of inhibition against the indicator strains tested, suggesting at least two different substances with distinct molecular weight. Conclusions, Significance and Impact of the Study: This is the first report on the production of antimicrobial substances in P. peoriae. Besides the antimicrobial inhibition capability, the strain NRRL BD-62 is also able to effectively fix molecular nitrogen, and produce chitinases and proteases as well, suggesting that further studies should be addressed to use P. peoriae strain NRRL BD-62 as a plant growth promoter and/or as a biocontrol agent in field experiments.

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“…In the present work, a strain of P. polymyxa EBL-06 was isolated from wheat phyllosphere, which could restrain the growth of the filamentous fungi. P. polymyxa strains have been proved to produce a wide variety of secondary metabolites, including different antibacterial and/or antifungal compounds (von der Weid et al, 2000). Therefore, the antagonistic effect of these strains upon microbial growth suggests a potential application as biological control agents.…”

Section: Introductionmentioning

confidence: 99%

Production of a newly isolated Paenibacillus polymyxa biocontrol agent using monosodium glutamate wastewater and potato wastewater

Gu¹,

Bai²,

Jin³

et al. 2010

Journal of Environmental Sciences

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A phyllosphere bacterial strain EBL-06 was isolated from wheat leaves. The morphology, cultural characteristics, phospholipid fatty acids, physiological and antagonistic fungus activities of this strain were investigated. A phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the relevant bacteria. The results showed that the isolate EBL-06 was a strain of Paenibacillus polymyxa; this strain performed a high level of antagonistic fungus activity toward a broad spectrum of phytopathogens, such as Botrytis cinerea, Cladosporium cucumerinum, Fusarium spp. The isolate EBL-06 can grow well using monosodium glutamate wastewater (MGW) and potato wastewater (PW) as culture medium. The maximum yield of 6.5 × 10 9 CFU/mL of the isolate EBL-06 anti-fungus biocontrol agent was reached in 15 hr cultivation at 28°C, pH 6.0-7.5 using the mixture of MGW and PW (1:9).

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Diversity of strains isolated from the rhizosphere of maize planted in Cerrado soil

Cited by 89 publications

References 54 publications

Effect of Primers Hybridizing to Different Evolutionarily Conserved Regions of the Small-Subunit rRNA Gene in PCR-Based Microbial Community Analyses and Genetic Profiling

Effect of Primers Hybridizing to Different Evolutionarily Conserved Regions of the Small-Subunit rRNA Gene in PCR-Based Microbial Community Analyses and Genetic Profiling

Antimicrobial activity of Paenibacillus peoriae strain NRRL BD-62 against a broad spectrum of phytopathogenic bacteria and fungi

Production of a newly isolated Paenibacillus polymyxa biocontrol agent using monosodium glutamate wastewater and potato wastewater

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