Diversity of Selected Lupinus angustifolius L. Genotypes at the Phenotypic and DNA Level with Respect to Microscopic Seed Coat Structure and Thickness

Abstract

The paper investigates seed coat characteristics (as a percentage of overall seed diameter) in Lupinus angustifolius L., a potential forage crop. In the study ten L. angustifolius genotypes, including three Polish cultivars, two Australian cultivars, three mutants originated from cv. ‘Emir’, and one Belarusian and one Australian breeding line were evaluated. The highest seed coat percentage was recorded in cultivars ‘Sonet’ and ‘Emir’. The lowest seed coat thickness percentage (below 20%) was noted for breeding lines 11257-19, LAG24 and cultivar ‘Zeus’ (17.87%, 18.91% 19.60%, respectively). Despite having low seed weight, the Australian line no. 11257-19 was characterized by a desirable proportion of seed coat to the weight of seeds. In general, estimation of the correlation coefficient indicated a tendency that larger seeds had thinner coats. Scanning Electron Microscopy images showed low variation of seed coat sculpture and the top of seeds covered with a cuticle. Most of the studied genotypes were characterized by a cristatepapillate seed coat surface, formed by elongated polygonal cells. Only breeding line no. 11267-19 had a different shape of the cells building the surface layer of the coat. In order to illustrate genetic diversity among the genotypes tested, 24 ISSR primers were used. They generated a total of 161 polymorphic amplification products in 10 evaluated narrow-leaved lupin genotypes.

Fatty Acid Composition, Phytochemistry, Antioxidant Activity on Seed Coat and Kernel of Paeonia ostii from Main Geographic Production Areas

“…In future studies, the breeding of new cultivars with lower seed coat rate would be a promising prospect in view of edible oil production. Similar results of seed coat rate have been obtained in Lupinus angustifolius [30], rapeseed [31], and beans [32], which would function as good references for tree peony breeding with the intent of producing higher oil content. In addition, the relatively high level of crude protein in seed kernel (20.21%) indicates that the seed could be included in food formulations as a source of protein after oil processing.…”

“…Then, 2.5 mL of the extraction solvent mixture (petroleum ether:ethyl ether, 1:1) was added and mixed for 5 min and then followed by centrifugation. The ether layer was transferred into a tube, washed with distilled water at least 3 times until it had Foods 2020, 9,30 4 of 20 a slightly less than neutral pH value, and then the water phase was removed and the ether layer evaporated under nitrogen gas flow.…”

Preliminary Genetic Map of a New Recombinant Inbred Line Population for Narrow-leafed Lupin (Lupinus angustifolius L.)

“…ISSR markers represent non_coding areas of the genome [34]. In the case of L. angustifolius such type of molecular system was important mostly in studies on the evaluation of genetic diversity in the collection [25]. However, that type of markers was also successfully used for map construction for other species [35][36][37].…”

“…In the first stage of the study 100 primers were used, 69 of them (Table S1) generated polymorphic products among parental forms and they were selected for genotyping mapping population. PCR and separation of amplification products were performed according to Clements et al [25].…”

The role of Cryptococcus laurentii and mycorrhizal fungi in the nutritional physiology of Lupinus angustifolius L. hosting N2-fixing nodules

“…The blue lupin, Lupinus angustifolius L., is a widelycultivated leguminous plant (Clements et al 2014;Kroc et al 2014) that has gained popularity as a crop over other lupins in the past few years due to its high grain yield and lower seed alkaloid content (Clements et al 2014). In addition to being cultivated for its protein rich grain, this plant is employed in crop rotation practices with cereals in Mediterranean climates (Jones 2001;McNeill and Fillery 2008;Wijayanto et al 2009).…”