Diversity of saproxylic basidiomycetes in <i>Quercus ilex</i> woodlands of central and insular Greece

Polemis, Elias; Dimou, D.; Fryssouli, Vassiliki; Zervakis, Georgios I.

doi:10.1080/11263504.2018.1492991

Cited by 4 publications

(3 citation statements)

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“…Due to indistinguishable morphology between Fmed and F. punctata, but the highlighted phylogenetic differences, previous isolates and findings attributed to F. punctata should be reconsidered as possibly assignable to Fmed (Fischer, 2002;Ciccarone et al, 2004;Fischer, 2006). Recent findings on P. punctatus and P. pseudopunctatus A. David Dequatre and Fiasson by Polemis et al (2019) and Markakis et al (2019) have reinforced that possible misidentification led to the underestimation of F. mediterranea incidence in the Mediterranean region.…”

Section: Introductionmentioning

confidence: 99%

Fomitiporia mediterranea M. Fisch., the historical Esca agent: a comprehensive review on the main grapevine wood rot agent in Europe

Moretti

Pacetti

Pierron

et al. 2021

Phytopathol. Mediterr.

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Fomitiporia mediterranea M. Fisch. (Fmed) is a basidiomycete first described in 2002, and was considered up to then as part of Fomitiporia punctata (P. Karst) Murrill. This fungus can degrade lignocellulosic biomass, causing white rot and leaving bleached fibrous host residues. In Europe Fmed is considered the main grapevine wood rot (Esca) agent within the Esca disease complex, which includes some of the most economically important Grapevine Trunk Diseases (GTDs). This review summarises and evaluates published research on Fmed, on white rot elimination by curettage or management by treatments with specific products applied to diseased grapevines, and on the relationship between wood symptoms and Grapevine Leaf Stripe Disease (GLSD) in the Esca disease complex. Information is also reviewed on the fungus biology, mechanisms of pathogenicity, and their possible relationships with external foliar symptoms of the Esca disease complex. Information on Fmed control strategies is also reviewed.

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Section: Introductionmentioning

confidence: 99%

Fomitiporia mediterranea M. Fisch., the historical Esca agent: a comprehensive review on the main grapevine wood rot agent in Europe

Moretti

Pacetti

Pierron

et al. 2021

Phytopathol. Mediterr.

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“…It should be considered that misidentification with P. punctatus has probably led to the underestimation of F. mediterranea in the Mediterranean area [85]. Analogously, Polemis et al [86] suggested that the relationship with P. pseudopunctatus A. David, Dequatre and Fiasson should be reconsidered as well, enclosing the latter in F. mediterranea clade. It may be observed that the P. pseudopunctatus is apparently more related to the South Mediterranean region and climates [87,88].…”

Section: Introductionmentioning

confidence: 99%

An Italian Research Culture Collection of Wood Decay Fungi

et al. 2020

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One of the main aims of the University of Pavia mycology laboratory was to collect wood decay fungal (WDF) strains in order to deepen taxonomic studies, species distribution, officinal properties or to investigate potential applications such as biocomposite material production based on fungi. The Italian Alps, Apennines and wood plains were investigated to collect Basidiomycota basidiomata from living or dead trees. The purpose of this study was to investigate the wood decay strains of the Mediterranean area, selecting sampling sites in North and Central Italy, including forests near the Ligurian and Adriatic seas, or near the Lombardy lakes. The isolation of mycelia in pure culture was performed according to the current methodology and the identity of the strains was confirmed by molecular analyses. The strains are maintained in the Research Culture Collection MicUNIPV of Pavia University (Italy). Among the 500 WDF strains in the collection, the most interesting isolates from the Mediterranean area are: Dichomitus squalens (basidioma collected from Pinus pinea), Hericium erinaceus (medicinal mushroom), Inocutis tamaricis (white-rot agent on Tamarix trees), Perenniporia meridionalis (wood degrader through Mn peroxidase) and P. ochroleuca. In addition, strains of species related to the Mediterranean climate (e.g., Fomitiporia mediterranea and Cellulariella warnieri) were obtained from sites with a continental-temperate climate.

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“…Abies cephalonica is an endemic fir species in Greece, and it occurs in the continental part of the country and in the islands of Evia and Kefalonia, at altitudes of 900-1,700 m. In addition, the sclerophyllous forests of Quercus ilex (holm oak) exist in the greater Mediterranean area only (Caudullo et al, 2017), and due to their value as biodiversity reserves they are included in the priority Habitats Directive 92/43/EEC (Annex I). As regards Greece in particular, Q. ilex is a rather common maquis element, especially in areas with relatively high precipitation; in Aegean islands, it appears in scattered localities, mostly as relict stands, at altitudes of 200-800 m. Although macrofungi in Greek forests dominated by either A. cephalonica or Q. ilex have been investigated for years and several hundred of plant-associated mushroom species were recorded (Pantidou, 1980;Zervakis et al, 2002;Dimou et al, 2008;Polemis et al, 2013Polemis et al, , 2014Polemis et al, , 2019, this approach which focuses on the collection and examination of visible sporocarps (basidiomata and ascomata), reveals just part of the existing diversity of Basidiomycota and (to an even lesser extent of) Ascomycota. Moreover, no data are available about other key elements of the forest microbiota (e.g., all other fungal groups as well as Bacteria), let alone of information pertinent to the relative abundance and functional diversity of soil microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the lignocellulose degradation potential of Mediterranean forests soil microbial communities through diversity and targeted functional metagenomics

et al. 2023

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The enzymatic arsenal of several soil microorganisms renders them particularly suitable for the degradation of lignocellulose, a process of distinct ecological significance with promising biotechnological implications. In this study, we investigated the spatiotemporal diversity and distribution of bacteria and fungi with 16S and Internally Trascribed Spacer (ITS) ribosomal RNA next-generation-sequencing (NGS), focusing on forest mainland Abies cephalonica and insular Quercus ilex habitats of Greece. We analyzed samples during winter and summer periods, from different soil depths, and we applied optimized and combined targeted meta-omics approaches aiming at the peroxidase-catalase family enzymes to gain insights into the lignocellulose degradation process at the soil microbial community level. The microbial communities recorded showed distinct patterns of response to season, soil depth and vegetation type. Overall, in both forests Proteobacteria, Actinobacteria, Acidobacteria were the most abundant bacteria phyla, while the other phyla and the super-kingdom of Archaea were detected in very low numbers. Members of the orders Agaricales, Russulales, Sebacinales, Gomphales, Geastrales, Hysterangiales, Thelephorales, and Trechisporales (Basidiomycota), and Pezizales, Sordariales, Eurotiales, Pleosporales, Helotiales, and Diaporthales (Ascomycota) were the most abundant for Fungi. By using optimized “universal” PCR primers that targeted the peroxidase-catalase enzyme family, we identified several known and novel sequences from various Basidiomycota, even from taxa appearing at low abundance. The majority of the sequences recovered were manganese peroxidases from several genera of Agaricales, Hysterangiales, Gomphales, Geastrales, Russulales, Hymenochaetales, and Trechisporales, while lignin -and versatile-peroxidases were limited to two to eight species, respectively. Comparisons of the obtained sequences with publicly available data allowed a detailed structural analysis of polymorphisms and functionally relevant amino-acid residues at phylogenetic level. The targeted metagenomics applied here revealed an important role in lignocellulose degradation of hitherto understudied orders of Basidiomycota, such as the Hysterangiales and Gomphales, while it also suggested the auxiliary activity of particular members of Proteobacteria, Actinobacteria, Acidobacteria, Verrucomicrobia, and Gemmatimonadetes. The application of NGS-based metagenomics approaches allows a better understanding of the complex process of lignocellulolysis at the microbial community level as well as the identification of candidate taxa and genes for targeted functional investigations and genetic modifications.

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Diversity of saproxylic basidiomycetes in Quercus ilex woodlands of central and insular Greece

Cited by 4 publications

References 20 publications

Fomitiporia mediterranea M. Fisch., the historical Esca agent: a comprehensive review on the main grapevine wood rot agent in Europe

Fomitiporia mediterranea M. Fisch., the historical Esca agent: a comprehensive review on the main grapevine wood rot agent in Europe

An Italian Research Culture Collection of Wood Decay Fungi

Evaluation of the lignocellulose degradation potential of Mediterranean forests soil microbial communities through diversity and targeted functional metagenomics

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