Diversity of rhizospheric and endophytic bacteria isolated from dried fruit of Ficus carica

Abid, Lamis; Smiri, Marwa; Federici, Ermanno; Lievens, Bart; Manaï, Mohamed; Yan, Yunjun; Sadfi‐Zouaoui, Najla

doi:10.1016/j.sjbs.2022.103398

Cited by 4 publications

(2 citation statements)

References 70 publications

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“…The samples (10 g/sample) were placed in a sterile stomacher bag containing sterile physiological saline solution (190 mL) and homogenized for two minutes. Further, the suspensions were serially diluted and plated onto NA medium [43].…”

Section: Bacteria Isolation From Citrullus Colocynthis Rhizosphere An...mentioning

confidence: 99%

Plant-Disease-Suppressive and Growth-Promoting Activities of Endophytic and Rhizobacterial Isolates Associated with Citrullus colocynthis

Al-Shuaibi,

Kazerooni,

Hussain

et al. 2023

Pathogens

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This study was conducted to investigate the antagonistic potential of endophytic and rhizospheric bacterial isolates obtained from Citrullus colocynthis in suppressing Fusarium solani and Pythium aphanidermatum and promoting the growth of cucumber. Molecular identification of bacterial strains associated with C. colocynthis confirmed that these strains belong to the Achromobacter, Pantoea, Pseudomonas, Rhizobium, Sphingobacterium, Bacillus, Sinorhizobium, Staphylococcus, Cupriavidus, and Exiguobacterium genera. A dual culture assay showed that nine of the bacterial strains exhibited antifungal activity, four of which were effective against both pathogens. Strains B27 (Pantoea dispersa) and B28 (Exiguobacterium indicum) caused the highest percentage of inhibition towards F. solani (48.5% and 48.1%, respectively). P. aphanidermatum growth was impeded by the B21 (Bacillus cereus, 44.7%) and B28 (Exiguobacterium indicum, 51.1%) strains. Scanning electron microscopy showed that the strains caused abnormality in phytopathogens’ mycelia. All of the selected bacterial strains showed good IAA production (>500 ppm). A paper towel experiment demonstrated that these strains improved the seed germination, root/shoot growth, and vigor index of cucumber seedlings. Our findings suggest that the bacterial strains from C. colocynthis are suppressive to F. solani and P. aphanidermatum and can promote cucumber growth. This appears to be the first study to report the efficacy of these bacterial strains from C. colocynthis against F. solani and P. aphanidermatum.

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Section: Bacteria Isolation From Citrullus Colocynthis Rhizosphere An...mentioning

confidence: 99%

Plant-Disease-Suppressive and Growth-Promoting Activities of Endophytic and Rhizobacterial Isolates Associated with Citrullus colocynthis

Al-Shuaibi,

Kazerooni,

Hussain

et al. 2023

Pathogens

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“…Currently, with the development of biochemistry and sequencing techniques, some new molecular biology techniques, such as PCR-RFLP techniques, DGGE analysis and metagenomic sequencing technologies, have brought great convenience to bacterial identification and microbial community structure ( Saini et al, 2013 ; Beghalem et al, 2017 ; Fadiji & Babalola, 2020 ; Abid et al, 2022 ). PCR-RFLP technology has unique advantages over metagenomic sequencing technology.…”

Section: Introductionmentioning

confidence: 99%

Diversity analysis of Populus euphratica endophytic bacteria in Tarim River Basin, China

Cheng,

Rahman

2023

PeerJ

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The bacterial diversity in Populus euphratica stem storage liquid samples grown in Shaya County and Yuli County of the Tarim River Basin was investigated. A culture-dependent (dilution spread plate method) and culture-independent method (PCR-RFLP technique) were used to identify the endophytic bacteria community structure and composition in P. euphratica in Tarim River Basin. Sixty-six bacterial strains were isolated from P. euphratica stem storage liquid samples on three agar media. The 16S rDNA gene was amplified and sequenced using bacterial universal primers. Phylogenetic analysis showed that the 66 strains belonged to three phyla (Firmicutes, Actinomycetes, and Gamma-Proteobacteria) and included 16 genera and 29 species. Among them, Pseudomonas (27.27%) and Bacillus (19.69%) were the dominant isolates. CGM-17 was a potentially new species of Pantoea. Restriction fragment length polymorphism of 16S rDNA gene amplified by polymerase chain reaction (PCR-RFLP) revealed 48 operational taxonomic units (OTUs). Phylogenetic analysis indicated that the 48 OTUs belonged to Firmicutes, Actinobacteria, Proteobacteria (α-, β-, γ-subgroup), Bacteroidetes, and Verrucomicrobia. Gamma-Proteobacteria was the dominant group, similarly to the culture-dependent method, accounting for 53% of the entire bacterial clone library. Our results indicate that P. euphratica endophytic bacteria diversity in the Tarim River Basin was rich, and the resources of endophytic bacteria were high. They provide valuable reference data and species resources for screening indigenous and functional strains of endophytic bacteria in P. euphratica.

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Isolation and identification of Rhizospheric and Endophytic Bacteria from Cucumber plants irrigated with wastewater: Exploring their roles in plant growth promotion and disease suppression

Shreshtha,

Raj,

Pal

et al. 2024

Current Research in Microbial Sciences

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Diversity of rhizospheric and endophytic bacteria isolated from dried fruit of Ficus carica

Cited by 4 publications

References 70 publications

Plant-Disease-Suppressive and Growth-Promoting Activities of Endophytic and Rhizobacterial Isolates Associated with Citrullus colocynthis

Plant-Disease-Suppressive and Growth-Promoting Activities of Endophytic and Rhizobacterial Isolates Associated with Citrullus colocynthis

Diversity analysis of Populus euphratica endophytic bacteria in Tarim River Basin, China

Isolation and identification of Rhizospheric and Endophytic Bacteria from Cucumber plants irrigated with wastewater: Exploring their roles in plant growth promotion and disease suppression

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