Diversity of rhizosphere associated entomopathogenic fungi of perennial herbs, shrubs and coniferous trees

Fisher, Joanna J.; Rehner, Stephen A.; Bruck, Denny J.

doi:10.1016/j.jip.2010.11.001

Cited by 92 publications

(45 citation statements)

References 37 publications

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“…Numerous investigations have relied only on this method of EPF isolation (Chandler et al 1997, Bidochka et al 1998, Ali-Shtayeh et al 2003, Meyling and Eilenberg 2006, Quesada-Moraga et al 2007, Sevim et al 2009, Fisher et al 2011, Muñiz-Reyes et al 2014, Pérez-González et al 2014, Fernández-Salas et al 2017, Gan and Wickings 2017, Kirubakaran et al 2018. The bait-insect Tenebrio molitor Linnaeus (Coleoptera: Tenebrionidae) has also been used solely in some studies (Sánchez-Peña et al 2011, Aguilera Sammaritano et al 2016.…”

Section: Introductionmentioning

confidence: 99%

Entomopathogenic fungi in Portuguese vineyards soils: suggesting a ‘Galleria-Tenebrio-bait method’ as bait-insects Galleria and Tenebrio significantly underestimate the respective recoveries of Metarhizium (robertsii) and Beauveria (bassiana)

Sharma

Oliveira

Torres

et al. 2018

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Entomopathogenic fungi (EPF) are the natural enemies of insect-pests. However, EPF recoveries can be influenced by the soil habitat-type(s) incorporated and/or the bait-insect(s) used. Galleria mellonella (GM) as bait-insect, i.e. 'Galleria-bait', is arguably the most common methodology, which is sometimes used solely, to isolate EPF from soils. Insect baiting using Tenebrio molitor (TM) has also been employed occasionally. Here 183 soils were used to estimate the functional diversity of EPF in Portuguese Douro vineyards (cultivated habitat) and adjacent hedgerows (semi-natural habitat), using the TM bait method. Moreover, to study the effect of insect baiting on EPF recovery, 81 of these 183 soil samples were also tested for EPF occurrences using the GM bait method. Twelve species were found in 44.26% ± 3.67% of the total of 183 soils. Clonostachys rosea f. rosea was found in maximum soils (30.05% ± 3.38%), followed by Beauveria bassiana (12.57% ± 2.37%), Purpureocillium lilacinum (9.29% ± 2.14%) and Metarhizium robertsii (6.01% ± 1.75%). Beauveria pseudobassiana (P < 0.001), C. rosea f. rosea (P = 0.006) and RESEARCH ARTICLELav Sharma et al. / MycoKeys 38: 1-23 (2018) 2 Cordyceps cicadae (P=0.023) were isolated significantly more from hedgerows, highlighting their sensitivities towards agricultural disturbances. Beauveria bassiana (P = 0.038) and M. robertsii (P = 0.003) were isolated significantly more using GM and TM, respectively. Principal component analysis revealed that M. robertsii was associated both with TM baiting and cultivated habitats, however, B. bassiana was slightly linked with GM baiting only. Ecological profiles of B. bassiana and P. lilacinum were quite similar while M. robertsii and C. rosea f. rosea were relatively distant and distinct. To us, this is the first report on (a) C. cicadae isolation from Mediterranean soils, (b) Purpureocillium lavendulum as an EPF worldwide; and (c) significant recoveries of M. robertsii using TM over GM. Overall, a 'Galleria-Tenebrio-bait method' is advocated to study the functional diversity of EPF in agroecosystems.

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Section: Introductionmentioning

confidence: 99%

Entomopathogenic fungi in Portuguese vineyards soils: suggesting a ‘Galleria-Tenebrio-bait method’ as bait-insects Galleria and Tenebrio significantly underestimate the respective recoveries of Metarhizium (robertsii) and Beauveria (bassiana)

Sharma

Oliveira

Torres

et al. 2018

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“…Recent studies have shown that some species, including Metarhizium robertsii (formerly known as M. anisopliae var. anisopliae) also colonize the rhizosphere (the layer of soil influenced by root metabolism) of specific plant types (13)(14)(15). This result was unexpected, and given the abundance of Metarhizium could have profound implications for plant biology.…”

mentioning

confidence: 99%

Local adaptation of an introduced transgenic insect fungal pathogen due to new beneficial mutations

Wang

O’Brien²,

Pava‐Ripoll³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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Genetically modified Metarhizium spp represent a major new arsenal for combating insect pests and insect-borne diseases. However, for these tools to be used safely and effectively, we need a much better understanding of their evolutionary potential and invasion ecology. In order to model natural as well as anthropogenic dispersal scenarios, we investigated evolutionary processes in a green fluorescent protein tagged strain of Metarhizium robertsii following transfer from a semitropical to a temperate soil community. Adaptive changes occurred over four years despite recurrent genetic bottlenecks and lack of recombination with locally well adapted strains. By coupling microarray-based functional analysis with DNA hybridizations we determined that expression of cell wall and stress response genes evolved at an accelerated rate in multiple replicates, whereas virulence determinants, transposons, and chromosome structure were unaltered. The mutable genes were enriched for TATA boxes possibly because they are larger mutational targets. In further field trials, we showed that the new mutations increased the fitness of M. robertsii in the new range by enhancing saprophytic associations, and these benefits were maintained in subsequent years. Consistent with selection being habitat rather than host specific, populations of an avirulent mutant cycled with seasons similarly to the wild type, whereas a mutant unable to adhere to plant roots showed a linear decrease in population. Our results provide a mechanistic basis for understanding postrelease adaptations, show that agents can be selected that lack gene flow and virulence evolution, and describe a means of genetically containing transgenic strains by disrupting the Mad2 gene.adaptive evolution | biocontrol | transgenic microbes | entomopathogenic fungi | soil fungi

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“…Metarhizium community in temperate North America appeared to be dominated by M. brunneum, M. robertsii and M. guizhouense (FISHER et al, 2011;WYREBEK et al, 2011;, with M. anisopliae a minor component or absent altogether. Our results corroborate other studies regarding the most frequent…”

Section: Discussionmentioning

confidence: 98%

“…distribution is more associated with habitats than with host insects as firstly hypothesized (BIDOCHKA et al, 2001;FISHER et al, 2011;WYREBEK et al, 2011;, which shows the importance to study ecological aspects of this fungi group in each crop system.…”

Section: Metarhizium Anisopliaementioning

confidence: 92%

Abundance, genetic diversity and persistence of <i>Metarhizium</i> spp. fungi from soil of strawberry crops and their potential as biological control agents against the two-spotted spider mite <i>Tetranychus urticae</i>

Castro¹

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Diversity of rhizosphere associated entomopathogenic fungi of perennial herbs, shrubs and coniferous trees

Cited by 92 publications

References 37 publications

Entomopathogenic fungi in Portuguese vineyards soils: suggesting a ‘Galleria-Tenebrio-bait method’ as bait-insects Galleria and Tenebrio significantly underestimate the respective recoveries of Metarhizium (robertsii) and Beauveria (bassiana)

Entomopathogenic fungi in Portuguese vineyards soils: suggesting a ‘Galleria-Tenebrio-bait method’ as bait-insects Galleria and Tenebrio significantly underestimate the respective recoveries of Metarhizium (robertsii) and Beauveria (bassiana)

Local adaptation of an introduced transgenic insect fungal pathogen due to new beneficial mutations

Abundance, genetic diversity and persistence of <i>Metarhizium</i> spp. fungi from soil of strawberry crops and their potential as biological control agents against the two-spotted spider mite <i>Tetranychus urticae</i>

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