Diversity of resistant determinants, virulence factors, and mobile genetic elements in Acinetobacter baumannii from India: A comprehensive in silico genome analysis

Kumkar, Shital N.; Kamble, Ekta E.; Chavan, Nikeeta S.; Dhotre, Dhiraj; Pardesi, Karishma

doi:10.3389/fcimb.2022.997897

Cited by 5 publications

(3 citation statements)

References 97 publications

(90 reference statements)

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“…A recent whole genome-based study on A. baumannii isolates (n=47) from India revealed the presence of as many as 79 types of ARGs of intrinsic and acquired nature [45]. Similar to our observations, high prevalence of sul2 and tetB among A. baumannii has been reported in previous studies from India [32,45], Pakistan [46] and Algeria [47].…”

Section: Discussionsupporting

confidence: 91%

“…This included genes conferring resistance to fluoroquinolones (qnrS and aac(6')-Ib-cr), aminoglycosides (strA, strB and aphA1-Iab), sulphonamides (sul1 and sul2) and tetracycline (tetB). A recent whole genome-based study on A. baumannii isolates (n=47) from India revealed the presence of as many as 79 types of ARGs of intrinsic and acquired nature [45]. Similar to our observations, high prevalence of sul2 and tetB among A. baumannii has been reported in previous studies from India [32,45], Pakistan [46] and Algeria [47].…”

Section: Discussionsupporting

confidence: 91%

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Genotyping of Acinetobacter baumannii isolates from a tertiary care hospital in Cochin, South India

Rajan,

Vijayan,

Puthiyottu Methal

et al. 2023

Access Microbiology

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Acinetobacter baumannii poses a significant challenge in healthcare settings across the globe, with isolates exhibiting carbapenem resistance at unprecedented rates. Here, we characterized a collection of A. baumannii isolates (n=64) recovered during the period September 2020 – November 2021 at a teaching hospital in Cochin, South India. The species identity of the isolates was confirmed with bla OXA-51-like PCR. The major carbapenemase determinants identified were bla OXA-23-like (45, 70.3 %) and bla NDM-1 (31, 48.4 %); co-occurrence of these genes was also observed in 27 (42.2 %) isolates. Other resistance genes identified included bla PER (34, 53.1 %), aac(6')-Ib-cr (42, 65.6 %), qnrS (25, 39.1 %), sul1 (32, 50 %), sul2 (33, 51.6 %), strA/strB (36, 56.3 %), aphA1-Iab (35, 54.7 %) and tetB (32, 50 %). Mapping PCR revealed the insertion element, ISAbaI upstream of bla OXA-23-like in all isolates possessing this gene. Concerning disinfectant resistance, all isolates carried the quaternary ammonium compound (QAC) resistance gene, qacEΔ1. Minimal inhibitory concentration (MIC) of benzalkonium chloride was high among the isolates and ranged from 8 to 128 µg ml−1. However, low MICs were observed for chlorhexidine and triclosan, with the majority (54, 80.6 %) of isolates showing an MIC of 2 µg ml−1 for chlorhexidine and all isolates exhibiting MICs of ≤0.125 µg ml−1 for triclosan. Further, all isolates were strong biofilm-producers, as assessed by the crystal violet-based microtitre plate assay. The ApaI-pulsed-field gel electrophoresis (PFGE) revealed the multi-clonal nature of the isolates, with 16 clusters and 16 unique pulsotypes identified at a cut-off of 80 %. In short, this study provides useful data on the molecular features of A. baumannii from this region, which could be helpful to assess the local epidemiology of this pathogen and also to devise infection control strategies.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Genotyping of Acinetobacter baumannii isolates from a tertiary care hospital in Cochin, South India

Rajan,

Vijayan,

Puthiyottu Methal

et al. 2023

Access Microbiology

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“…MGEs play a pivotal role in the horizontal transfer of genes (Kumkar et al., 2022). Plasmids are particularly important MGEs, serve as crucial carriers for the transmission of ARGs (Zhu et al., 2023).…”

Section: Discussionmentioning

confidence: 99%

Effects of antibiotic resistance genes on health risks of rivers in habitat of wild animals under human disturbance – based on analysis of antibiotic resistance genes and virulence factors in microbes of river sediments

Hu,

Ren,

Liang

et al. 2024

Ecology and Evolution

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Studying the ecological risk of antibiotic resistance genes (ARGs) to wild animals from human disturbance (HD) is an important aspect of “One Health”. The highest risk level of ARGs is reflected in pathogenic antibiotic‐resistant bacteria (PARBs). Metagenomics was used to analyze the characteristics of PARBs in river sediments. Then, the total contribution of ARGs and virulence factors (VFs) were assessed to determine the health risk of PARBs to the rivers. Results showed that HD increased the diversity and total relative abundance of ARG groups, as well as increased the kinds of PARBs, their total relative abundance, and their gene numbers of ARGs and VFs. The total health risks of PARBs in wild habitat group (CK group), agriculture group (WA group), grazing group (WG group), and domestic sewage group (WS group) were 0.067 × 10−3, −1.55 × 10−3, 87.93 × 10−3, and 153.53 × 10−3, respectively. Grazing and domestic sewage increased the health risk of PARBs. However, agriculture did not increase the total health risk of the rivers, but agriculture also introduced new pathogenic mechanisms and increased the range of drug resistance. More serious was the increased transfer risk of ARGs in the PARBs from the rivers to wild animals under agriculture and grazing. If the ARGs in the PARBs are transferred from the rivers under HD to wild animals, then wild animals may face severe challenges of acquiring new pathogenic mechanisms and developing resistance to antibiotics. Further analysis showed that the total phosphorus (TP) and dissolved organic nitrogen (DON) were related to the risk of ARGs. Therefore, controlling human emissions of TP and DON could reduce the health risk of rivers.

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Exploration of potential hit compounds targeting 1-deoxy-d-xylulose 5-phosphate reductoisomerase (IspC) from Acinetobacter baumannii: an in silico investigation

Zia,

Jain,

Muthukumaran

et al. 2024

3 Biotech

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Diversity of resistant determinants, virulence factors, and mobile genetic elements in Acinetobacter baumannii from India: A comprehensive in silico genome analysis

Cited by 5 publications

References 97 publications

Genotyping of Acinetobacter baumannii isolates from a tertiary care hospital in Cochin, South India

Genotyping of Acinetobacter baumannii isolates from a tertiary care hospital in Cochin, South India

Effects of antibiotic resistance genes on health risks of rivers in habitat of wild animals under human disturbance – based on analysis of antibiotic resistance genes and virulence factors in microbes of river sediments

Exploration of potential hit compounds targeting 1-deoxy-d-xylulose 5-phosphate reductoisomerase (IspC) from Acinetobacter baumannii: an in silico investigation

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