Diversity of Pseudomonas plasmids: To what extent?

Boronin, A. M.

doi:10.1111/j.1574-6968.1992.tb05740.x

Cited by 17 publications

(13 citation statements)

References 12 publications

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“…The identity of inserted DNA may be mainly of plasmid origin, since P. aeruginosa is known to frequently harbor those elements (5). The 95-kb plasmid which is integrated between hemL and pilA was found free in clonal variants of clone C. In strain C itself, it was shown to be stably integrated during several rounds of subcultivation (data not shown).…”

Section: Discussionmentioning

confidence: 96%

Comparative genome mapping of Pseudomonas aeruginosa PAO with P. aeruginosa C, which belongs to a major clone in cystic fibrosis patients and aquatic habitats

1996

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A physical and genetic map was constructed for Pseudomonas aeruginosa C. Mainly, two-dimensional methods were used to place 47 SpeI, 8 PacI, 5 SwaI, and 4 I-CeuI sites onto the 6.5-Mb circular chromosome. A total of 21 genes, including the rrn operons and the origin of replication, were located on the physical map. Comparison of the physical and genetic map of strain C with that of the almost 600-kb-smaller genome of P. aeruginosa reference strain PAO revealed conservation of gene order between the two strains. A large-scale mosaic structure which was due to insertions of blocks of new genetic elements which had sizes of 23 to 155 kb and contained new SpeI sites was detected in the strain C chromosome. Most of these insertions were concentrated in three locations: two are congruent with the ends of the region rich in biosynthetic genes, and the third is located in the proposed region of the replication terminus. In addition, three insertions were scattered in the region rich in biosynthetic genes. The arrangement of the rrn operons around the origin of replication was conserved in C, PAO, and nine other examined independent strains.

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Section: Discussionmentioning

confidence: 96%

Comparative genome mapping of Pseudomonas aeruginosa PAO with P. aeruginosa C, which belongs to a major clone in cystic fibrosis patients and aquatic habitats

1996

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“…The Pseudomonas IncP-9 group consists of both degradative, for example pWW0 ("117 kb), SAL ("68 kb) and NAH ("81 kb), and resistance plasmids, for example R2 ("73 kb) and pMG18 ("100 kb) The GenBank accession number for the sequence reported in this paper is AF078924. (Bayley et al, 1979 ;Boronin, 1992b ;Lehrbach et al, 1983). The host range of at least some of these plasmids is clearly wider than Pseudomonas species because pWW0, when tagged with the transposon Tn401 has been shown to transfer to, and be maintained in, Escherichia coli (Benson & Shapiro, 1978).…”

Section: Introductionmentioning

confidence: 99%

The replication and stable-inheritance functions of IncP-9 plasmid pM3 The GenBank accession number for the sequence reported in this paper is AF078924.

et al. 2000

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Little is known of the transfer and maintenance machinery of the IncP-9 plasmids, which are found in Pseudomonas spp. and include both degradative and resistance plasmids. One such plasmid, pM3, which confers resistance to streptomycin and tetracycline, was found repeatedly in Pseudomonas species from numerous locations in Belarus. pM3 has a broad host range, but is unable to replicate in enterobacteria at 37 SC and above. A mini derivative, pMT2, was constructed by partial PstI digestion and ligation with a fragment encoding Km R . The complete sequence of pMT2 was determined. Analysis of its 8526 bp of pM3 DNA revealed several ORFs whose predicted polypeptide products were found to have similarity to previously analysed proteins involved in plasmid replication (rep gene), transfer (mpf ; mating-pair formation gene) and stable maintenance (par, mrs genes). The organization of these genes showed similarity to several plasmid systems including the Ti and pSYM plasmids as well as IncP-1 plasmids. Subcloning narrowed down the region required for replication, and identified the putative rep gene and putative par promoter region as able to express incompatibility. rep deletion mutants were lost from the cell line, and expression of the rep gene was shown to be controlled by negative autoregulation. A pMT2 derivative with an insertion between the rep and par genes showed very weak, if any, ability to replicate autonomously, suggesting that plasmid maintenance may depend on a close interaction of rep and par functions.

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“…A major purpose of our current work is to provide information to help rectify this defect. We have therefore set out to sequence the archetypes of the various Pseudomonas plasmid groups (7,24).Plasmid Rms149 was discovered in a clinical strain, Pseudomonas aeruginosa Ps142, from Frankfurt in Germany (37); it confers multiple antibiotic resistances. It has since been shown to replicate in Escherichia coli after mobilization by an IncP-1 plasmid (21,22).…”

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The IncP-6 Plasmid Rms149 Consists of a Small Mobilizable Backbone with Multiple Large Insertions

et al. 2005

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Plasmid Rms149, the archetype of Pseudomonas plasmid incompatibility group IncP-6, was identified in Pseudomonas aeruginosa as an agent conferring resistance to streptomycin, sulfanilamide, gentamicin, and carbenicillin in 1975. It has been classed as a broad-host-range plasmid due to its ability to replicate in both Escherichia coli (where it is designated IncG) and Pseudomonas species, although both species are ␥-proteobacteria. To provide reference information on this Inc group, we have determined the complete sequence of Rms149 and found that, although the genome comprises 57,121 bp, it is essentially a small mobilizable plasmid carrying multiple mobile elements, which make up 79% (>45 kb) of its genome. A replicon has been identified which encodes a single polypeptide with moderate identity to other replication proteins. The region encoding this protein can replicate in Pseudomonas putida and E. coli. This sequence is directly downstream of a putative partitioning region highly similar to that of pRA2. A functional IncQ-type mobilization region is also present. Thus, the backbone appears to be a novel combination of modules already identified in other plasmid systems. Analysis of the segments that fall outside this core of stable inheritance and transfer functions show that this plasmid has been subject to multiple insertion events and that the plasmid appears to carry a considerable load of DNA that no longer should be phenotypically advantageous. The plasmid therefore functions not just as a vehicle for spread of selective traits but also as a store for DNA that is not currently under selection.The key features of a plasmid are the ability to replicate autonomously and to be maintained in a cell lineage without a high rate of segregational loss. An optional but frequently encountered property is the ability to transfer or to be mobilized from one bacterium to another. From these properties it follows that genes that become associated with a plasmid may spread rapidly from one genetic background to another. Some plasmids appear to consist of nothing more than functions that confer the above core abilities, and these have been termed cryptic plasmids.To succeed as such a selfish element, a plasmid must be stable and confer minimal burden on its host, or at least overcome competitive losses by horizontal transfer (42). Conversely, a plasmid carrying genes that promote the growth of its host, relative to competitor bacteria, will benefit by increased propagation. However, since maintenance of a plasmid is generally found to place a metabolic or phenotypic load on the cell, universally useful genes will be selectively reacquired by the chromosome over evolutionary time. Therefore, successful plasmids typically carry payload genes, favorable in some environments but not others (12), or genes with a transient plasmid association that are spreading through a microbial community. In passing through different strains or species, plasmids are exposed to different genetic contexts and provide the opportunity for comb...

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Diversity of Pseudomonas plasmids: To what extent?

Cited by 17 publications

References 12 publications

Comparative genome mapping of Pseudomonas aeruginosa PAO with P. aeruginosa C, which belongs to a major clone in cystic fibrosis patients and aquatic habitats

Comparative genome mapping of Pseudomonas aeruginosa PAO with P. aeruginosa C, which belongs to a major clone in cystic fibrosis patients and aquatic habitats

The replication and stable-inheritance functions of IncP-9 plasmid pM3 The GenBank accession number for the sequence reported in this paper is AF078924.

The IncP-6 Plasmid Rms149 Consists of a Small Mobilizable Backbone with Multiple Large Insertions

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