Diversity of organophosphorus pesticide-degrading bacteria in a polluted soil and conservation of their organophosphorus hydrolase genes

Abstract

Seven methyl parathion-degrading bacteria were isolated from a long-term methyl parathion contaminated soil and were found to belong to the genera Pseudaminobacter, Achromobacter, Brucella, and Ochrobactrum. Southern blot analysis using an mpd gene probe revealed that their hydrolase genes were similar to the mpd gene from Plesiomonas sp. strain M6 and were all located on the chromosome. Gene libraries were constructed from genomic DNA of each of the 7 organophosphorus pesticide-degrading bacteria, and their mpd genes were cloned and sequenced. Sequence analysis revealed that their hydrolase genes were conserved, and that the G+C content of the mpd genes were distinctly different from that of the chromosome-located 16S rRNA gene, suggesting that the mpd gene could be transferred and expressed among a variety of bacterial hosts.

Biodegradation of Chlorpyrifos (CP) by a Newly Isolated Naxibacter sp. Strain CY6 and Its Ability to Degrade CP in Soil

“…Rani and Lalitha-Kumari (1994) found that Pseudomonas putida could hydrolyze methyl parathion and use ρ-nitrophenol as a sole source of carbon. Zhang et al (2005) isolated seven MPT-degrading bacteria from soil that had long-term MPT contamination. Zhang et al (2006) reported a fenitrothion-degrading strain, Burkholderia sp.…”

“…The CP or OP insecticides degrading bacteria was isolated previously (Sethunathan and Yoshida, 1973;Shelton, 1988;Rani and Lalitha-Kumari, 1994;Singh et al, 2004;Yang et al, 2005Yang et al, , 2006Zhang et al, 2005Zhang et al, , 2006Li et al, 2007;Xu et al, 2008;Anwar et al, 2009;Cho et al, 2009;Wang et al, 2012) but this is the first report of OP insecticides degradation by Naxibacter species. In addition, the several researchers has identified the various genes (opd) and enzymes (OP hydrolase) involved in degradation of OP insecticides (Fu et al, 2004;Liu et al, 2005;Zhang et al, 2006;Li et al, 2007;Yang et al, 2008;Islam et al, 2010).…”

Microbial degradation of pesticide: A review

“…M6 strain (Zhongli et al, 2001). The gene for the organophosphorus hydrolase has 996 nucleotides, a typical promoter sequence of the promoter TTGCAA N17 TATACT from E. coli (Zhang et al, 2005). Table 5 shows the most studied isolates of microorganisms capable of degrading pesticide with their several genes.…”

“…This property can be accelerated by applying genetic engineering techniques. The unique Singh and Walker, 2006;Serdar et al, 1982.Somara andSiddavattam, 1995;Horne et al, 2002;Cheng et al, 1996;Cheng et al, 1997;Zhongli et al, 2001;Zhang et al, 2005;Chen and Mulchandani (1998);Chen et al, 1999;Amitai et al, 1998;Liu et al, 2001;Liu et al, 2004;Liu et al, 2001 Agrobacterium radiobacter Alteromonas sp. …”

Pesticides in the Environment: Impacts and its Biodegradation as a Strategy for Residues Treatment

“…Another gene coding for organophosphates hydrolase, mpd, described originally in Plesiomonas sp. (Zhongli et al, 2001), has also been found in other genera like Achromobacter, Pseudaminobacter, Ochrobactrum and Brucella and is located in the chromosome (Zhang et al, 2005). Other important bacterial genera able to degrade organophosphates include Burkholderia (Zhang, et al, 2006) and Hyphomicrobium (Wang et al, 2010).…”