Diversity of nitrogen-fixing rhizobacteria associated with sugarcane: a comprehensive study of plant-microbe interactions for growth enhancement in Saccharum spp.

Abstract

Background: Nitrogen is an essential element for sugarcane growth and development and is generally applied in the form of urea often much more than at recommended rates, causing serious soil degradation, particularly soil acidification, as well as groundwater and air pollution. In spite of the importance of nitrogen for plant growth, fewer reports are available to understand the application and biological role of N 2 fixing bacteria to improve N 2 nutrition in the sugarcane plant.

Complete Genome Sequence of Enterobacter roggenkampii ED5, a Nitrogen Fixing Plant Growth Promoting Endophytic Bacterium With Biocontrol and Stress Tolerance Properties, Isolated From Sugarcane Root

“…The interactions between beneficial microbes of the host plant might play an essential part in the achievement of microbial bioinoculant for improving the production of crops, but there is no strong thought on the entire role of colonization on the plant microbiome. The GFP pictures showed a signal of the occurrence of E. roggenkampii in the intercellular regions also as cell aggregates or isolated single cells in the root of sugarcane (Li et al, 2017;Singh et al, 2020). The SEM images confirmed of selected inoculated strain E. roggenkampii in stem and roots specified that the forms of adherence in sugarcane, and similar observation were also found in other endophytic strains like Paenibacillus polymyxa, Rhizobium sp.…”

“…Therefore, this study explored almost all PGP traits like nitrogen fixation, IAA, siderophore, phosphate, ACC, HCN, and ammonia production of the selected strains isolated from sugarcane root. Several other studies also showed that all PGP traits comprising bacterial strains from sugarcane used as bio-inoculants and increased sugarcane yield (Li et al, 2017;Singh et al, 2020). The genome of E. roggenkampii covers several genes contributing to plantbeneficial roles, such as ACC, siderophore, ammonia, IAA production, phosphate metabolism, and nitrogen fixation.…”

“…All endophytic strains were assessed for their in vitro antifungal activities against Fusarium moniliforme, Fusarium cubense, Botrytis cinereal, Ceratocystis paradoxa, and Sporisorium scitamineum with the slight modification of Singh et al (2020) method on NA plus PDA (1:1) medium. These all fungal pathogens were obtained from Agriculture College, Guangxi University, Nanning.…”

“…After 72 h plantlets were taken away and washed with autoclaved water. Micro-propagated cultivated sugarcane plantlets were cut into a small section and observed by a CLSM (Leica DMI 6000, Germany) (Singh et al, 2020). Sugarcane plant samples (stem and root tissues) were selected for the SEM analysis, both samples were cut into small pieces by knife and fixed in glutaraldehyde solution (Catalog G1102, Servicebio) overnight at 4 • C. The samples were washed three times with distilled water and dehydrated in ethanol 30, 50, 70, 90, 95, and 100% for 15 min and finally isoamyl acetate for 15 min.…”

Nodule and Root Zone Microbiota of Salt-Tolerant Wild Soybean in Coastal Sand and Saline-Alkali Soil

“…PGPR can fix nitrogen via non-symbiotic bacteria, thus enabling a biologically inactive form of nitrogen to be readily used by organisms (Singh et al, 2020;Soares et al, 2020). Since diverse microbial communities use this process to fulfill their nitrogen demands (Liu et al, 2012), the phylogenetic diversity of nifH genes, a molecular marker of nitrogen fixation (Gaby et al, 2018), has been examined extensively in the microbiota of various plants and invertebrates, including humans (Yamada et al, 2007;Xiao et al, 2010;Liu et al, 2012;Igai et al, 2016).…”