Diversity of Mycobacterium tuberculosis Complex Lineages Associated with Pulmonary Tuberculosis in Southwestern, Uganda

Micheni, Lisa Nkatha; Kassaza, Kennedy; Kinyi, Hellen; Ntulume, Ibrahim; Bazira, Joel

doi:10.1155/2021/5588339

Cited by 5 publications

(8 citation statements)

References 33 publications

(53 reference statements)

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“…Tropical Medicine and Health Oryema et al Tropical Medicine and Health (2021) 49:92 one-third of the world's population currently infected and more than 1.3 million deaths per year [1,2]. It is a contagious disease caused by Mycobacterium tuberculosis.…”

Section: Open Accessmentioning

confidence: 99%

“…Tuberculosis (TB) is an ancient communicable disease that has been in existence over the past millennia and remains a major global public health burden, with one-third of the world’s population currently infected and more than 1.3 million deaths per year [ 1 , 2 ]. It is a contagious disease caused by Mycobacterium tuberculosis .…”

Section: Introductionmentioning

confidence: 99%

“…It usually attacks the lungs, although it can also spread to other parts of the body, and is transmitted through coughing and sneezing [ 2 ]. In most Asian and some African countries, the prevalence of TB per 100,000 population peaks among those aged 35‒54 years [ 1 ]. This covers the productive age group, which consequently affects the growth of any country.…”

Section: Introductionmentioning

confidence: 99%

“…This covers the productive age group, which consequently affects the growth of any country. Globally, an estimated 10 million people developed active TB disease in 2019, with 1.4 million TB deaths [ 1 ]. Progress in achieving the United Nations (UN) General Assembly End TB targets remains slow.…”

Section: Introductionmentioning

confidence: 99%

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Ethnobotanical plants used in the management of symptoms of tuberculosis in rural Uganda

et al. 2021

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Background Tuberculosis (TB) caused by Mycobacterium tuberculosis is the 13th leading cause of death worldwide. The emergence of multidrug-resistant TB (MDR-TB) poses a major health security threat. Plants have traditionally been used as a source of medicine, since olden days and 80% of the communities in Africa still rely on herbal medicines for their healthcare. In many parts of Uganda, some plants have shown ethno-pharmacological prospects for the treatment of TB, and yet they have not been fully researched. Aim This study aimed to document plant species used traditionally by the herbalists and non-herbalist communities of Kitgum and Pader districts for managing symptoms of TB. Methods An ethnobotanical study was carried out in 42 randomly selected villages in Kitgum and Pader districts between August 2020 and January 2021. Information was obtained by administering semi-structured questionnaires to 176 respondents identified by snowball and random sampling methods. Data were analysed and presented using descriptive statistics and Informant Consensus Factor (ICF). Results Overall, only 27% of the respondents were knowledgeable about plants used for managing symptoms of TB. Nine plant species belonging to six families (Mimosaceae, Apiaceae, Lamiaceae, Rutaceae, Loganiaceae and Rubiaceae) were used to manage symptoms of TB. The most representative family was Rutaceae with three species, followed by Rubiaceae (two species) and the rest of the families were represented by one species each. The most frequently recorded species were Steganotaenia araliacea Hochst. (8.5%), Gardenia ternifolia Schumach. & Thonn (6.8%) and Albizia adianthifolia (Schum.) W.Wight (6.8%). Most of the medicinal plants were trees, and roots (69%) were the most frequently plant part used, followed by the bark (16%) and leaves (15%). The most common method of preparation was by pounding and mixing concoction with water. The administration of the concoctions was mostly done orally. Conclusions The results established the existence of few medicinal plants for managing symptoms of TB among the Acholi communities which could be used in developing new, effective plant-based antimycobacterial drugs. The few plants mentioned might face conservation threats due to exploitations of the roots. Phytochemical and toxicological studies are recommended to identify active compounds responsible for antimycobacterial activity.

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Section: Open Accessmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ethnobotanical plants used in the management of symptoms of tuberculosis in rural Uganda

et al. 2021

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“…Recording 1.3 million deaths per year across the globe, Tuberculosis (TB) is one of the major public health concerns, affecting primarily the respiratory system [ 1 ]. It is a contagious disease primarily attacking the lungs and is caused by a single infectious agent, Mycobacterium tuberculosis (Mtb) [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Anti Mtb Medicinal Plants Database (AMMPDB): A curated database of Indian anti-tubercular medicinal plants

Kanneganti¹,

Mina²,

Singh³

et al. 2023

Journal of Ayurveda and Integrative Medicine

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Detection of Mycobacterium tuberculosis multiple strains in sputum samples from patients with pulmonary tuberculosis in south western Uganda using MIRU-VNTR

Micheni

Kassaza

Kinyi

et al. 2022

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Infections with multiple strains of Mycobacterium tuberculosis are now widely recognized as a common occurrence. Identification of patients infected with multiple strains provides both insight into the disease dynamics and the epidemiology of tuberculosis. Analysis of Mycobacterial Interspersed Repetitive Unit-Variable-Number Tandem Repeats (MIRU-VNTR) has been shown to be highly sensitive in detecting multiple M. tuberculosis strains even in sputum. The goal of this study was to identify cases of multiple M. tuberculosis strain infections among patients diagnosed with pulmonary tuberculosis in Southwestern Uganda and assessment of factors associated with multiple strain infections. DNA extracted directly from 78 sputum samples, each from an individual patient, was analyzed using the standard 24 loci MIRU-VNTR typing. Five (6.4%) of the 78 patients were infected with multiple strains of M. tuberculosis with all of them being the newly diagnosed cases while two-thirds of them were co-infected with HIV. Exact regression analysis projected that the natives were more likely to harbor multiple strains (OR; 0.981, 95% CI 0–7.926) as well as those with a high microbial load (OR; 0.390, 95% CI 0–3.8167). Despite these findings being not statistically significant due to the small sample size, this points to a critical component of disease dynamics that has clinical implications and emphasizes a need for a study using a larger cohort. It is also essential to study the potential factors associated with higher risk of exposure to newly diagnosed and HIV positive patients at the community level. In addition, our ability to detect multiple M. tuberculosis strains using the standard 24 loci MIRU-VNTR typing especially with allelic diversity in loci 2059 and 3171, which are excluded from the 15-locus MIRU-VNTR, lead us to recommend the use of this genotyping technique, especially in areas with tuberculosis endemicity similar to this study.

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Diversity of Mycobacterium tuberculosis Complex Lineages Associated with Pulmonary Tuberculosis in Southwestern, Uganda

Cited by 5 publications

References 33 publications

Ethnobotanical plants used in the management of symptoms of tuberculosis in rural Uganda

Ethnobotanical plants used in the management of symptoms of tuberculosis in rural Uganda

Anti Mtb Medicinal Plants Database (AMMPDB): A curated database of Indian anti-tubercular medicinal plants

Detection of Mycobacterium tuberculosis multiple strains in sputum samples from patients with pulmonary tuberculosis in south western Uganda using MIRU-VNTR

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