Diversity of Mycobacteriaceae from aquatic environment at the São Paulo Zoological Park Foundation in Brazil

Romagnoli, Camila Lopes; Pellegrino, Kátia Cristina Machado; Silva, Natalia Maria; Brianesi, Urze Adomaitis; Leão, Sylvia Cardoso; Rabello, Michelle Christiane da Silva; Viana-Niero, Cristina

doi:10.1371/journal.pone.0227759

Cited by 6 publications

(5 citation statements)

References 55 publications

(48 reference statements)

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“…None of these organisms were found in water samples during our study so we were unable to evaluate their growth from environmental sources. However, at least some of these species including M. haemophilum [1], M. neoaurum [31], M. phlei [32] and M. xenopi [33] have been isolated from water samples by others. Only M. haemophilum and M. xenopi were noted by the CDC as environmental organisms of concern for HAIs.…”

Section: Plos Onementioning

confidence: 99%

Evaluation of a new culture medium for isolation of nontuberculous mycobacteria from environmental water samples

Alexander¹,

Furlong²,

Baron³

et al. 2021

PLoS ONE

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Nontuberculous mycobacteria (NTM) are waterborne pathogens commonly found in building water systems where they are a primary concern to vulnerable patient populations and can cause severe disease. The recovery of NTM from environmental samples can be a laborious undertaking and current pre-treatment methods and selective media lack sensitivity. We explored the use of the highly selective Rapidly Growing Mycobacteria (RGM) medium for culturing NTM from environmental water samples compared to existing methods. In total, 223 environmental water samples, including potable and non-potable water, were cultured for NTM using three culture media. In addition to direct culture on RGM medium, each sample was cultured on Middlebrook 7H10 medium and Mitchison 7H11 medium after pre-treatment with 0.2M KCl-HCl. Additionally, 33 distinct species of NTM were inoculated onto RGM medium and 7H10 medium in parallel to directly compare their growth. The use of RGM medium alone without pre-treatment provided a sensitivity (91%) comparable to that offered by culture on both 7H10 and 7H11 with acid pretreatment (combined sensitivity; 86%) with significantly less overgrowth and interference from other organisms on RGM medium. The average concentration of NTM observed on RGM medium alone was comparable to or greater than the NTM concentration on either medium alone or combined. Thirty-three species were examined in parallel and all tested strains of 27 of these species successfully grew on RGM medium, including 19 of 21 from the CDC’s healthcare-associated infections species list. RGM medium was successful at recovering environmental NTM without a pre-treatment, greatly reducing labor and materials required to process samples. Simplification of culture processing for environmental NTM will allow for a better assessment of their presence in building water systems and the potential for reduced exposure of susceptible populations.

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Section: Plos Onementioning

confidence: 99%

Evaluation of a new culture medium for isolation of nontuberculous mycobacteria from environmental water samples

Alexander¹,

Furlong²,

Baron³

et al. 2021

PLoS ONE

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“…In this sense, studies are essential for advancing our knowledge of microbial diversity, which has implications for fields as diverse as medicine, ecology, industry and environmental sciences. In our previous study, a large sampling of mycobacteria from water samples from the São Paulo Zoological Park was evaluated and the results suggested the presence of a putative new species ( Romagnoli et al, 2020 ). From these, five isolates were selected for further characterization regarding their taxonomic position.…”

Section: Discussionmentioning

confidence: 99%

“…Strains MYC017, MYC098, MYC101, MYC123 and MYC340 were isolated from sewage at the São Paulo Zoological Park in Brazil ( Romagnoli et al, 2020 ). Bacteria were cultivated in Middlebrook 7H10 medium supplemented with 0.5% glycerol and 10% OADC (oleic acid, albumin, dextrose, and catalase - Becton Dickinson, Franklin Lakes, USA) at 30°C for up to 10 days.…”

Section: Methodsmentioning

confidence: 99%

“…In a previous study, we assessed a large diversity of bacteria belonging to the Mycobacteriaceae family isolated from different sources of water samples in a zoo in the state of São Paulo - Brazil, as well as a variety of potential new species ( Romagnoli et al, 2020 ). Here, we present five of these isolates as candidates for being new species belonging to MTC, after being characterized by biochemical and cultural tests, matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) and genomic features.…”

Section: Introductionmentioning

confidence: 99%

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Description of new species of Mycobacterium terrae complex isolated from sewage at the São Paulo zoological park foundation in Brazil

Romagnoli,

Conceição,

Machado

et al. 2024

Front. Microbiol.

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Five mycobacterial isolates from sewage were classified as members of the genus Mycobacterium but presented inconclusive species assignments. Thus, the isolates (MYC017, MYC098, MYC101, MYC123 and MYC340) were analyzed by phenotypical, biochemical, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and genomic features to clarify their taxonomic position. Phenotypic analysis and biochemical tests did not distinguish these isolates from other non-pigmented mycobacteria. In contrast, MALDI-TOF MS analysis showed that isolates were not related to any previously described Mycobacterium species. Comparative genomic analysis showed values of ANI and dDDH between 81.59–85.56% and 24.4–28.8%, respectively, when compared to the genomes of species of this genus. In addition, two (MYC101 and MYC123) presented indistinguishable protein spectra from each other and values of ANI = 98.57% and dDDH = 97.3%, therefore being considered as belonging to the same species. Phylogenetic analysis grouped the five isolates within the Mycobacterium terrae complex (MTC) but in a specific subclade and separated from the species already described and supported by 100% bootstrap value, confirming that they are part of this complex but different from earlier described species. According to these data, we propose the description of four new species belonging to the Mycobacterium genus: (i) Mycobacterium defluvii sp. nov. strain MYC017T (= ATCC TSD-296T = JCM 35364T), (ii) Mycobacterium crassicus sp. nov. strain MYC098T (= ATCC TSD-297T = JCM 35365T), (iii) Mycobacterium zoologicum sp. nov. strain MYC101T (= ATCC TSD-298T = JCM 35366T) and MYC123 (= ATCC BAA-3216 = JCM 35367); and (iv) Mycobacterium nativiensis sp. nov. strain MYC340T (= ATCC TSD-299T = JCM 35368T).

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“…Strains MYC038, MYC040, MYC211, MYC221, and MYC223, identified as M. austroafricanum , were isolated from sewage and a lake at São Paulo Zoological Park Foundation in Brazil [ 33 ]. Bacteria were grown on solid Middlebrook 7H10 media supplemented with 10% OADC (oleic acid, albumin, dextrose, and catalase) (Becton Dickinson, Franklin Lakes, NJ, USA) at 30 °C for 5 days.…”

Section: Methodsmentioning

confidence: 99%

Multi-Approach Characterization of Novel Pyrene-Degrading Mycolicibacterium austroafricanum Isolates Lacking nid Genes

Silva,

Romagnoli,

Santiago

et al. 2023

Microorganisms

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Polycyclic aromatic hydrocarbons (PAHs) are chemical compounds that are widespread in the environment, arising from the incomplete combustion of organic material, as well as from human activities involving petrol exploitation, petrochemical industrial waste, gas stations, and environmental disasters. PAHs of high molecular weight, such as pyrene, have carcinogenic and mutagenic effects and are considered pollutants. The microbial degradation of PAHs occurs through the action of multiple dioxygenase genes (nid), which are localized in genomic island denominate region A, and cytochrome P450 monooxygenases genes (cyp) dispersed in the bacterial genome. This study evaluated pyrene degradation by five isolates of Mycolicibacterium austroafricanum using 2,6-dichlorophenol indophenol (DCPIP assay), gas chromatography/mass spectrometry (CG/MS), and genomic analyses. Two isolates (MYC038 and MYC040) exhibited pyrene degradation indexes of 96% and 88%, respectively, over a seven-day incubation period. Interestingly, the genomic analyses showed that the isolates do not have nid genes, which are involved in PAH biodegradation, despite their ability to degrade pyrene, suggesting that degradation may occur due to the presence of cyp150 genes, or even genes that have not yet been described. To the best of our knowledge, this is the first report of isolates without nid genes demonstrating the ability to degrade pyrene.

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Diversity of Mycobacteriaceae from aquatic environment at the São Paulo Zoological Park Foundation in Brazil

Cited by 6 publications

References 55 publications

Evaluation of a new culture medium for isolation of nontuberculous mycobacteria from environmental water samples

Evaluation of a new culture medium for isolation of nontuberculous mycobacteria from environmental water samples

Description of new species of Mycobacterium terrae complex isolated from sewage at the São Paulo zoological park foundation in Brazil

Multi-Approach Characterization of Novel Pyrene-Degrading Mycolicibacterium austroafricanum Isolates Lacking nid Genes

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