Diversity of Multi-Drug Resistant Avian Pathogenic Escherichia coli (APEC) Causing Outbreaks of Colibacillosis in Broilers during 2012 in Spain

Solà-Ginés, Marc; Cameron-Veas, Karla; Badiola, Ignacio; Dolz, Roser; Majó, Natàlia; Dahbi, Ghizlane; Viso, Susana; Mora, Azucena; Blanco, Jorge; Piedra-Carrasco, Nuria; González-López, Juan José; Migura-García, Lourdes

doi:10.1371/journal.pone.0143191

Cited by 95 publications

(87 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar to other studies worldwide, the majority of the FEC isolates were phylogenetic group C, A and B1 (Hiki et al, 2014;Jakobsen et al, 2010;Sola-Gines et al, 2015). In this current study, fifty percent of the CEC isolates belonged to phylogenetic group A, which is typically identified as a commensal, non-pathogenic E. coli (Clermont et al, 2013).…”

Section: Discussionsupporting

confidence: 86%

“…The majority of the sequence types (ST10, ST57 ST 224, ST354 and S624) identified in the current study from FQ and ESC resistant isolates have been previously described and are globally distributed amongst humans as well as animals (Fernandes et al, 2016;Kim et al, 2011;Maluta et al, 2014;Sola-Gines et al, 2015;Zhao et al, 2014). ST2705 and ST6053 are host restricted and have been previously isolated from poultry and wild birds (Dahms et al, 2015;Day et al, 2016;Jones-Dias et al, 2015).…”

Section: Discussionmentioning

confidence: 60%

“…A number of studies showed that APEC is found in the surrounding environment around farms (Anza et al, 2014;Cortes et al, 2010;Sola-Gines et al, 2015). The reported overlap between APEC strains isolated from farm workers clothes, hands and boots, and poultry farms, suggest that people may act as a significant vehicle for the introduction and spread of APEC and other pathogens to the poultry farms from the external environment (Belanger et al, 2011;Cho et al, 2012;Johnson et al, 2008b;Lynne et al, 2012;Zhu Ge et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…In Australia, there is limited knowledge of the antimicrobial susceptibility of APEC to guide veterinarians in developing successful prevention and treatment schemes. Numbers of overseas studies have reported geographical variations in the antimicrobial susceptibility of APEC (Alvarez-Fernandez et al, 2013;Dheilly et al, 2013;Sola-Gines et al, 2015). The geographical variation in susceptibility of APEC highlights the importance of regional knowledge to control, prevent and treat avian colibacillosis (Aarestrup, 2005).…”

Section: Chapter 1: Literature Reviewmentioning

confidence: 99%

“…However, APEC significantly differs in their phylogenetic assignment as they cluster predominantly in phylogenetic group A (Sola-Gines et al, 2015;Zhu Ge et al, 2014). Caution has to be taken when comparing studies that have used different classification schemes.…”

Section: Phylogenetic Classificationmentioning

confidence: 99%

See 4 more Smart Citations

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

View full text Add to dashboard Cite

Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

show abstract

Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Chapter 1: Literature Reviewmentioning

confidence: 99%

Section: Phylogenetic Classificationmentioning

confidence: 99%

See 3 more Smart Citations

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

View full text Add to dashboard Cite

show abstract

Extraintestinal Pathogenic Escherichia coli

Biran

Ron

2018

Current Topics in Microbiology and Immunology

View full text Add to dashboard Cite

Extraintestinal pathogenic E. coli (ExPEC) present a major clinical problem that has emerged in the past years. Most of the infections are hospital or community-acquired and involve patients with a compromised immune system. The infective agents belong to a large number of strains of different serotypes that do not cross react. The seriousness of the infection is due to the fact that most of the infecting bacteria are highly antibiotic resistant. Here, we discuss the bacterial factors responsible for pathogenesis and potential means to combat the infections.

show abstract

New Concepts on Domestic and Wild Reservoirs and Transmission of E. coli and Its Environment

Bentancor

Crivelli

Piccini

et al. 2023

View full text Add to dashboard Cite

Diversity of Multi-Drug Resistant Avian Pathogenic Escherichia coli (APEC) Causing Outbreaks of Colibacillosis in Broilers during 2012 in Spain

Cited by 95 publications

References 50 publications

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Extraintestinal Pathogenic Escherichia coli

New Concepts on Domestic and Wild Reservoirs and Transmission of E. coli and Its Environment

Contact Info

Product

Resources

About