Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes

Abstract

Aims: The aim of this study was to determine the diversity of moderately halophilic bacteria with hydrolase activities. Methods and Results: Screening bacteria from different hypersaline environments in South Spain led to the isolation of a total of 122 moderately halophilic bacteria able to produce different hydrolases (amylases, DNases, lipases, proteases and pullulanases). These bacteria are able to grow optimally in media with 5-15% salts and in most cases up to 20-25% salts. In contrast to strains belonging to previously described species, that showed very little hydrolase activities, environmental isolates produced a great variety of hydrolases. These strains were identified as members of the genera: Salinivibrio (55 strains), Halomonas (25 strains), Chromohalobacter (two strains), Bacillus-Salibacillus (29 strains), Salinicoccus (two strains) and Marinococcus (one strain), as well as eight nonidentified isolates. Conclusions: Moderately halophilic bacteria are a source of hydrolytic enzymes such as amylases, DNases, lipases, proteases and pullulanases. Significance and Impact of the Study: Although most culture collection strains are not able to produce hydrolases, it has been shown that environmental isolates can produce these potentially biotechnological important enzymes.

Antibiosis and dark-pigments secretion by the phytopathogenic and environmental fungal species after interaction in vitro with a Bacillus subtilis isolate

“…Biochemical tests were performed for citrate utilization; nitrate reduction; growth on mannitol and growth in 6.5 and 10% NaCl; production of H 2 S, indole, methyl red, Voges-Proskauer (VP); and hydrolysis of esculin (Foldes et al, 2000, Reva et al, 1995, Winn et al, 2006. Enzymatic tests were performed to detect the production of amylase, ornithinedecarboxylase, oxidase, catalase, urease, lecithinase, DNase, lipase, caseinase and haemolysins (Winn et al, 2006, Sanchez-Porro et al, 2003. The growth of the bacterial strain on TSA and tryptic soy broth (TSB, Difco), pH 7.3, and SDA, pH 5.6, was observed at different temperatures (4,20,25,37,42, and 55º C) over intervals of 24 and 48 h.…”

Isolation and Screening of Protease Enzyme Producing Bacteria from Cheese at Dilla University, Ethiopia

“…They accelerate the velocity of the reaction without necessarily initiating it (Oyeleke and Oduwole, 2009). Extracellular hydrolytic enzymes such as amylases, proteases, lipases, DNases, pullulanases, and xylanases have quite diverse potential usages in different areas such as food industry, feed additive, biomedical sciences, and chemical industries (Sanchez-Porro et al, 2003). Protease refers to a group of enzymes whose catalytic function is to hydrolyze proteins.…”

Comparison of Bacterial Biodiversity and Enzyme Production in Three Hypersaline Lakes; Urmia, Howz-Soltan and Aran-Bidgol

“…A few studies have been carried out concerning microbial diversity and extracellular enzymes production in other saline habitats in the world. For example, Sanchez-Porro et al [14], showed the abundance of five hydrolytic enzymes including amylase, protease, lipase, DNase, pullulanase by moderately halophilic bacteria from saltern in Spain; Rohban et al [2], investigated screening of halophilic bacteria producing nine extracellular hydrolyses from Howz-Soltan Lake, Iran, Makhdoumi et al [15], investigated diversity of nine hydrolytic enzymes in haloarchaeal strains from Aran-Bidgol Lake, Iran and finally Kumar et al [31] investigated screening and isolation of halophilic bacteria producing three industrially important enzymes. The aim of the present study was to analyze the ability of moderate and extreme halophilic bacteria to produce nine different extracellular hydrolases from Urmia Lake, Aran-Bidgol playa and Howz-Soltan playa.…”

“…The isolation of moderate and extreme halophiles that are able to produce hydrolytic enzymes will provide the possibility to have optimal activities at different salt concentrations that could be useful in some industrial processes [2,14,15].…”