“…The higher variability in STR systems observed in Brangus Ibagé as compared to the other two 682 Bovine LEPR gene polymorphisms breeds probably results from its crossbreeding composition. High levels of STR genetic diversity in the BI herd has already been described (Almeida et al, 2003(Almeida et al, , 2007Duarte et al, 2005;Oliveira et al, 2005). The Charolais herd presented the highest variability concerning the T945M polymorphism.…”
The genetic diversity of a single nucleotide polymorphism (SNP) at the exon 20 (T945M) of the leptin receptor gene (LEPR) and of three short tandem repeats (STRs BM7225, BMS694, and BMS2145) linked to LEPR was investigated in three beef cattle herds (Brangus Ibagé, Charolais, and Aberdeen Angus). A cheap and effective new method to analyze the T945M polymorphism in cattle populations was developed and the possible role of these polymorphisms in reproduction and weight gain of postpartum cows was evaluated. High levels of genetic diversity were observed with the average heterozygosity of STRs ranging from 0.71 to 0.81. No significant association was detected between LEPR markers and reproductive parameters or daily weight gain. These negative results suggest that the LEPR gene polymorphisms, at least those herein described, do not influence postpartum cows production.
“…The higher variability in STR systems observed in Brangus Ibagé as compared to the other two 682 Bovine LEPR gene polymorphisms breeds probably results from its crossbreeding composition. High levels of STR genetic diversity in the BI herd has already been described (Almeida et al, 2003(Almeida et al, , 2007Duarte et al, 2005;Oliveira et al, 2005). The Charolais herd presented the highest variability concerning the T945M polymorphism.…”
The genetic diversity of a single nucleotide polymorphism (SNP) at the exon 20 (T945M) of the leptin receptor gene (LEPR) and of three short tandem repeats (STRs BM7225, BMS694, and BMS2145) linked to LEPR was investigated in three beef cattle herds (Brangus Ibagé, Charolais, and Aberdeen Angus). A cheap and effective new method to analyze the T945M polymorphism in cattle populations was developed and the possible role of these polymorphisms in reproduction and weight gain of postpartum cows was evaluated. High levels of genetic diversity were observed with the average heterozygosity of STRs ranging from 0.71 to 0.81. No significant association was detected between LEPR markers and reproductive parameters or daily weight gain. These negative results suggest that the LEPR gene polymorphisms, at least those herein described, do not influence postpartum cows production.
“…However, Duarte et al (2005) did not find any significant effect of microsatellite markers in Brangus Ibagé females (ILSTS027, MBO22 and BM4325) that could be linked to the follicle-stimulating hormone (FSH) genes, sub-unit β (FSHβ) on PPP, while IP has been proved to be positively influenced by the BM4325 marker. The authors indicate the use of the BM4325 marker for the FSH gene in selection based on markers aimed at improvement of the reproductive performance of the bovine females, suggesting, however, the confirmation of the information obtained within other cattle breeds.…”
ABSTRACT. The purpose of the present research was to investigate the effects of polymorphisms of luteinizing hormone receptor (LHR) and follicle-stimulating hormone receptor (FSHR) genes, evaluated by polymerase chain reaction-restriction fragment length polymorphism in EuropeanZebu composite beef heifers from six different breed compositions. The polymorphism site analysis from digestion with HhaI and AluI restriction endonucleases allowed the genotype identification for LHR (TT, CT and CC) and FSHR (GG, CG and CC) genes. A high frequency of heterozygous animals was recorded in all breed compositions for both genes, except in two compositions for LHR. The probability of pregnancy (PP) at first breeding was used to evaluate the polymorphism effect on sexual precocity. The PP was analyzed as a binary trait, with a value of 1 (success) assigned to heifers that were diagnosed pregnant by rectal palpation and a value of 0 (failure) assigned to those that were not pregnant at that time. Heterozygous heifers showed a higher pregnancy rate (67 and 66% for LHR and FSHR genes, respectively), but no significant effects were observed for the genes studied (P = 0.9188 and 0.8831 for LHR and FSHR, respectively) on the PP. These results do not justify the inclusion of LHR and FSHR restriction fragment length polymorphism markers in selection programs for sexual precocity in beef heifers. Nevertheless, these markers make possible the genotype characterization and may be used in additional studies to evaluate the genetic structure in other bovine populations.
“…This synthetic breed was created by the Brazilian Agricultural Research Corporation (EMBRAPA Pecuária Sul, Bagé, RS, Brasil). Details of the population could be found in DUARTE et al (2005). Genomic DNA was extracted from total blood by the method of MILLER et al (1988).…”
In the detection phase of a bovine marker assisted selection program, this paper investigated the genetic variability of three microsatellites on the chromosome 18 (BTA 18). The possible associations between genotypes or alleles of these markers versus weight at first calving and a lifetime calving interval (as indicators of reproductive performance) were evaluated in a beef cattle herd (5/8 Aberdeen Angus x 3/8 Nelore). Eleven alleles were detected in TGLA227 and ILSTS002 and three in BMS3004, the most frequent being TGLA227*79, ILSTS002*133, ILSTS002*135 and BMS3004*129. Polymorphic information content ranged from 0.41 to 0.84, while heterozygosity ranged from 49% to 86%, with an average value of 77%. The association analyses performed between genotype classes for the genetic markers versus weight at first calving indicated no significant result. Also, no correlation was observed between calving interval (CI) and TGLA227 genotypes. However, positive associations were detected between ILSTS002 and BMS3004 and CI. Animals carrying at least one ILSTS002*135 allele presented a CI about 39 days longer than the individuals with other genotypes; animals heterozygous for BMS3004 presented a CI about 35 days shorter than the homozygous. On these grounds, it can be concluded that these markers can be useful as an aid to fertility selection, in this herd.
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