Diversity of microsatellite markers in the populations of Picea asperata originating from the mountains of China

Wang, Yuhua; Luo, Jianxun; Xue, Xudong; Korpelainen, Helena; Li, Chunyang

doi:10.1016/j.plantsci.2004.10.002

Cited by 26 publications

(23 citation statements)

References 45 publications

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“…Such excesses of heterozygosity within populations might be the result of a high level of outbreeding and extensive population mixing at the local scale. The level of genetic differentiation among populations (G ST = 0.373) was much higher than that of most other forest tree species investigated using SSR markers (e.g., MAGUIRE et al, 2002;WANG et al, 2005), or that of the genus Populus based on isozyme (AGNES and FRANCOIS, 1996) and SSR markers (IMBERT and LEFÈVRE, 2003), but equal to that detected in P. cathayana based on RAPD markers (LI et al, 1997).…”

Section: Discussionmentioning

confidence: 79%

“…Therefore, abundant species, including many presently rare and endangered species have been preserved in this region. During the long evolutionary process, changes in distributions and adaptations to the harsh conditions have taken place (YU et al, 2003;WANG et al, 2005). Previous studies have showed that geographical isolation, complex and variable climate conditions are the main factors contributing to the genetic composition of plant populations (GEHRING and LINHART, 1992;STRAUS et al, 1992;YEH et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Population Genetic Survey of Populus cathayana Originating From Southeastern Qinghai-Tibetan Plateau of China Based on SSR Markers

Peng

Chen

et al. 2005

Silvae Genetica

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In this study, the genetic diversity of Populus cathayana Rehd was investigated using microsatellite markers. In a total of 150 individuals collected from six natural populations in the southeastern part of the Qinghai-Tibetan Plateau in China, a high level of microsatellite polymorphism was detected. At the seven investigated microsatellite loci, the number of alleles per locus ranged from 5 to 16, with a mean of 11.3, the observed heterozygosities across populations ranged from 0.408 to 0.986, with a mean of 0.792, and the expected heterozygosities across populations ranged from 0.511 to 0.891, with a mean of 0.802. The proportion of genetic differentiation among populations accounted for 37.3% of the whole genetic diversity. The presence of such a high level of genetic diversity could be attributed to the features of the species and the habitats where the sampled populations occur: The southeastern part of the Qinghai-Tibetan Plateau is regarded as the natural distribution and variation center of the genus Populus in China. Variation in environmental conditions and selection pressures in different populations, and topographic dispersal barriers could be factors associated with the high level of genetic differentiation found among populations. The populations possessed significant heterozygosity excesses, which may be due to extensive population mixing at the local scale. The cluster analysis showed that the populations are not strictly grouped according to their geographic distances but the habitat characteristics also influence the divergence pattern. In addition, we suggest that population SHY should be regarded as an ecologically divergent species of P. cathayana.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Population Genetic Survey of Populus cathayana Originating From Southeastern Qinghai-Tibetan Plateau of China Based on SSR Markers

Peng

Chen

et al. 2005

Silvae Genetica

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“…Wright's analysis of hierarchical F-statistics (Fst, ratio of gene diversities of heterozygosities) (Wright 1978) and gene divergence (Gst) (Slatkin and Barton 1989) were used to assess genetic differentiation between populations. Flow parameters of gene Nm (Fst) (Wang et al 2005) and Nm (Gst) (Francis and Yang 1993) were measured indirectly from Fst and Gst, respectively. SPSS (version 10.3) was applied to conduct cluster analysis among the accessions.…”

Section: Discussionmentioning

confidence: 99%

Using SSR markers to determine the population genetic structure of wild apricot (Prunus armeniaca L.) in the Ily Valley of West China

Chen

Zheng³

et al. 2006

Genet Resour Crop Evol

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Genetic structure of three wild populations (Xinyuan, Gongliu and Daxigou) of apricot in the Ily Valley, Xinjiang Uygur Autonomous Region of China, was investigated with microsatellite (simple sequence repeat, SSR) markers. The higher polymorphism and greater transportability of these markers between Prunus species proved SSR markers were much efficient for conducting genetic diversity studies in wild apricot. Nei's gene diversity (He) and Shannon's index of diversity (I) were 0.287 and 0.458, respectively. This indicated that the wild apricot in the Ily Valley still maintained a relatively high level of diversity. The Gst of 0.137 and Fst of 0.164 revealed that genetic variation mainly resided among individuals within populations (83.6-86.3%). Population differentiation could also be found according to the distribution of SSR alleles between the populations. Mantel test showed the genetic distance between populations was significantly correlated to the geographical distance. The modest amount of gene flow (2.684) would reduce the disjunction between wild apricots. The long-distance dispersal of pollen by insects was probably the main way of gene flow between populations. Based on the study of population genetic structure, an effective conservation strategy of the species was discussed.

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“…It has high levels of alleles per polymorphic locus (A ¼ 19:8) and allelic richness (A R ¼ 11:9) and average levels of expected heterozygosity (H E ¼ 0:711) and population differentiation (F ST ¼ 0:131) relative to other conifers assessed with nuclear microsatellite markers (Al-Rabab'ah and Williams 2002; Boys et al 2005;Wang et al 2005;Karhu et al 2006;Potter et al 2008). We observed no significant changes in genetic diversity as P. oocarpa migrated into Central America; by contrast, its subtropical cousin Pinus maximinoi exhibited reduced genetic diversity with distance from its evolutionary center in central Mexico when assessed with RAPD markers (Dvorak et al 2002).…”

Section: Genetic Diversitymentioning

confidence: 99%

Genetic Diversity and Gene Exchange inPinus oocarpa, a Mesoamerican Pine with Resistance to the Pitch Canker Fungus (Fusarium circinatum)

Dvorak¹,

Potter

Hipkins

et al. 2009

International Journal of Plant Sciences

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Eleven highly polymorphic microsatellite markers were used to determine the genetic structure and levels of diversity in 51 natural populations of Pinus oocarpa across its geographic range of 3000 km in Mesoamerica. The study also included 17 populations of Pinus patula and Pinus tecunumanii chosen for their resistance or susceptibility to the pitch canker fungus based on previous research. Seedlings from all 68 populations were screened for pitch canker resistance, and results were correlated to mean genetic diversity and collection site variables. Results indicate that P. oocarpa exhibits average to above-average levels of genetic diversity (A ¼ 19:82, A R ¼ 11:86, H E ¼ 0:711) relative to other conifers. Most populations were out of Hardy-Weinberg equilibrium, and a high degree of inbreeding was found in the species (F IS ¼ 0:150). Bayesian analysis grouped P. oocarpa into four genetic clusters highly correlated to geography and distinct from P. patula and P. tecunumanii. Historic gene flow across P. oocarpa clusters was observed (N m ¼ 1:1-2:7), but the most pronounced values were found between P. oocarpa and P. tecunumanii (low-altitude provenances) in Central America (N m ¼ 9:7). Pinus oocarpa appears to have two main centers of diversity, one in the Eje Transversal Volcánico in central Mexico and the other in Central America. Introgression between P. oocarpa and P. tecunumanii populations appears to be common. Pinus oocarpa populations showed high resistance to pitch canker (stemkill 3%-8%), a disease that the species has presumably coevolved with in Mesoamerica. Resistance was significantly correlated to the latitude, longitude, and altitude of the collection site but not to any genetic-diversity parameters or degree of admixture with P. tecunumanii.

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Diversity of microsatellite markers in the populations of Picea asperata originating from the mountains of China

Cited by 26 publications

References 45 publications

Population Genetic Survey of Populus cathayana Originating From Southeastern Qinghai-Tibetan Plateau of China Based on SSR Markers

Population Genetic Survey of Populus cathayana Originating From Southeastern Qinghai-Tibetan Plateau of China Based on SSR Markers

Using SSR markers to determine the population genetic structure of wild apricot (Prunus armeniaca L.) in the Ily Valley of West China

Genetic Diversity and Gene Exchange inPinus oocarpa, a Mesoamerican Pine with Resistance to the Pitch Canker Fungus (Fusarium circinatum)

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