Diversity of methicillin-resistant coagulase-negative Staphylococcus spp. and methicillin-resistant Mammaliicoccus spp. isolated from ruminants and New World camelids

Schauer, Bernhard; Szostak, Michael P.; Ehricht, Ralf; Monecke, Stefan; Feßler, Andrea T.; Schwarz, Štefan; Spergser, Joachim; Krametter-Frötscher, Reinhild; Loncaric, Igor

doi:10.1016/j.vetmic.2021.109005

Cited by 19 publications

(27 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, we describe the insertion of a novel 27.6-kb genomic island, RI fosY , carrying the fosY gene. The structure of the island and the fosY gene were found to be similar to that in other species, such as M. sciuri and S. saprophyticus associated with livestock [ 30–32 ], implying that RI fosY may originate and recombine in the microbiome in livestock and then integrate into CC1 S. aureus . Therefore, RI fosY facilitates cross-species gene transfer, and the transfer of RI fosY to other clones may also be possible.…”

Section: Discussionmentioning

confidence: 89%

The novel fosfomycin resistance gene fosY is present on a genomic island in CC1 methicillin-resistant Staphylococcus aureus

Chen

Sun

et al. 2022

Emerging Microbes & Infections

View full text Add to dashboard Cite

Fosfomycin has gained attention as a combination therapy for methicillin-resistant Staphylococcus aureus infections. Hence, the detection of novel fosfomycin-resistance mechanisms in S. aureus is important. Here, the minimal inhibitory concentrations (MICs) of fosfomycin in CC1 methicillin-resistant S. aureus were determined. The pangenome analysis and comparative genomics were used to analyse CC1 MRSA. The gene function was confirmed by cloning the gene into pTXΔ. A phylogenetic tree was constructed to determine the clustering of the CC1 strains of S. aureus. We identified a novel gene, designated fosY , that confers fosfomycin resistance in S. aureus . The FosY protein is a putative bacillithiol transferase enzyme sharing 65.9–77.5% amino acid identity with FosB and FosD, respectively. The function of fosY in decreasing fosfomycin susceptibility was confirmed by cloning it into pTXΔ. The pTX- fosY transformant exhibited a 16-fold increase in fosfomycin MIC. The bioinformatic analysis showed that fosY is in a novel genomic island designated RI fosY (for “resistance island carrying fosY ”) that originated from other species. The global phylogenetic tree of ST1 MRSA displayed this fosY -positive ST1 clone, originating from different regions, in the same clade. The novel resistance gene in the fos family, fosY , and a genomic island, RI fosY , can promote cross-species gene transfer and confer resistance to CC1 MRSA causing the failure of clinical treatment. This emphasises the importance of genetic surveillance of resistance genes among MRSA isolates.

show abstract

Section: Discussionmentioning

confidence: 89%

The novel fosfomycin resistance gene fosY is present on a genomic island in CC1 methicillin-resistant Staphylococcus aureus

Chen

Sun

et al. 2022

Emerging Microbes & Infections

View full text Add to dashboard Cite

show abstract

“…Except one S. hyicus strain, whose MLST scheme has not yet been developed, MLST was performed on all S. aureus , S. epidermidis , and M. sciuri isolates as described previously ( Enright et al, 2000 ; Schauer et al, 2021 ; Thomas et al, 2007 ). For MLST analyses, internal fragments of seven housekeeping genes from each strain were PCR amplified and sequenced.…”

Section: Methodsmentioning

confidence: 99%

“…For MLST analyses, internal fragments of seven housekeeping genes from each strain were PCR amplified and sequenced. The seven genes amplified from each species of staphylococci are: arcC, aroE, gmk, glpF, pta, tpi, and yqiL for S. aureus ( Enright et al, 2000 ); arcC, aroE, gtr, pyrR, mutS, yqiL, and tpiA for S. epidermidis ( Thomas et al, 2007 ); ack, aroE, glpK, ftsZ, gmk, pta1, and tpiA for M. sciuri ( Schauer et al, 2021 ). The alleles and sequence types (STs) of each staphylococcal species were assigned according to the MLST databases ( https://www.pubmlst.org/ ).…”

Section: Methodsmentioning

confidence: 99%

Profiles of Non-aureus Staphylococci in Retail Pork and Slaughterhouse Carcasses: Prevalence, Antimicrobial Resistance, and Genetic Determinant of Fusidic Acid Resistance

Yang¹,

Lee²,

Kim³

et al. 2022

Food Sci Anim Resour

View full text Add to dashboard Cite

As commensal colonizers in livestock, there has been little attention on staphylococci, especially non-aureus staphylococci (NAS), contaminating meat production chain. To assess prevalence of staphylococci in retail pork and slaughterhouse carcass samples in Korea, we collected 578 samples from Korean slaughterhouses (n = 311) and retail markets (n = 267) for isolation of staphylococci and determined antimicrobial resistance phenotypes in all the isolates. The presence of and prevalence of fusB-family genes (fusB, fusC, fusD, and fusF) and mutations in fusA genes were examined in fusidic acid resistant isolates. A total of 47 staphylococcal isolates of 4 different species (S. aureus, n = 4; S. hyicus, n = 1; S. epidermidis, n = 10; M. sciuri, n = 32) were isolated. Fusidic acid resistance were confirmed in 9/10 S. epidermidis and all of the 32 Mammaliicoccus sciuri (previously Staphylococcus sciuri) isolates. Acquired fusidic acid resistance genes were detected in all the resistant strains; fusB and fusC in S. epidermidis and fusB/C in M. sciuri. MLST analysis revealed that ST63 (n =10, 31%) and ST30 (n =8, 25%) genotypes were most prevalent among fusidic acid resistant M. sciuri isolates. In conclusion, the high prevalence of fusB-family genes in S. epidermidis and M. sciuri strains isolated from pork meat indicated that NAS might act as a reservoir for fusidic acid resistance gene transmissions in pork production chains.

show abstract

“…Commercially available software ( ClinProTools 3.0 software, Bruker Billerica) was used to uncover differences between spectra which can be used as biomarkers. Schauer et al [74] collected nasal swabs from 723 cattle, calves, goats, sheep, llamas, and alpacas were collected in Vienna, Austria. Isolates of staphylococci or mammaliicocci grown on selective media were obtained and species were verified using MALDI-TOF.…”

Section: Streptococcus Salivarius Streptococcus Equinus Andmentioning

confidence: 99%

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in veterinary medicine: Recent advances (2019–present)

Thompson

2022

Vet World

View full text Add to dashboard Cite

Matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry (MS) has become a valuable laboratory tool for rapid diagnostics, research, and exploration in veterinary medicine. While instrument acquisition costs are high for the technology, cost per sample is very low, the method requires minimal sample preparation, and analysis is easily conducted by end-users requiring minimal training. Matrix-assisted laser desorption ionization-time-of-flight MS has found widespread application for the rapid identification of microorganisms, diagnosis of dermatophytes and parasites, protein/lipid profiling, molecular diagnostics, and the technique demonstrates significant promise for 2D chemical mapping of tissue sections collected postmortem. In this review, an overview of the MALDI-TOF technique will be reported and manuscripts outlining current uses of the technology for veterinary science since 2019 will be summarized. The article concludes by discussing gaps in knowledge and areas of future growth.

show abstract

Diversity of methicillin-resistant coagulase-negative Staphylococcus spp. and methicillin-resistant Mammaliicoccus spp. isolated from ruminants and New World camelids

Cited by 19 publications

References 32 publications

The novel fosfomycin resistance gene fosY is present on a genomic island in CC1 methicillin-resistant Staphylococcus aureus

The novel fosfomycin resistance gene fosY is present on a genomic island in CC1 methicillin-resistant Staphylococcus aureus

Profiles of Non-aureus Staphylococci in Retail Pork and Slaughterhouse Carcasses: Prevalence, Antimicrobial Resistance, and Genetic Determinant of Fusidic Acid Resistance

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in veterinary medicine: Recent advances (2019–present)

Contact Info

Product

Resources

About