Diversity of &lt;i&gt;Magnetospirillum&lt;/i&gt; Sp. from the Southern Coast of India

Jacob, Jobin John; Revathy, Theerthagiri; Jayasri, M. A.; Suthindhiran, K.

doi:10.18520/cs/v111/i1/177-183

Cited by 6 publications

(5 citation statements)

References 28 publications

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“…with several classes of Proteobacteria such as Alpha, Beta, and Gammaproteobacteria [55]. They can make use of the earth's magnetic field to accurately locate environments with a reduced amount of oxygen, (as they are micro-aerophilic in nature) especially in freshwaters, using a special organelle called magnetosomes [56].…”

Section: Discussionmentioning

confidence: 99%

Microbiome Diversity Analysis of the Bacterial Community in Idah River, Kogi State, Nigeria

Adedire¹,

Jimoh²,

Kashim-Bello³

et al. 2022

AiM

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The analysis of bacterial diversity in aquatic systems particularly in rivers, lakes, and streams can provide useful data on the effect of anthropogenic activities on such water bodies to humans and fishes. Idah River, the focal point of this study, is an offshoot of the two major Nigerian rivers characterized by observed human activities and pollution sources. Water samples were collected from four designated sites and assessed for their bacterial assemblages and structure, using PacBio Single-Molecule Real-Time (SMRT) sequencing technology. The full length of the 16S rRNA gene was sequenced, and Amplicon Sequence Variants were generated using the DADA2 workflow optimised for PacBio long-read amplicons in Rstudio. A total of 8751 high-quality reads obtained were taxonomically classified as 24 phyla, 42 classes, 84 orders, 125 families, 156 genera, and 106 species. Taxonomical composition revealed Proteobacteria as the most abundant phyla across all sample sites. At the genera level, Azospira (57.03%) was the most dominant ASV in Docking Point A, while Acinetobacter (66.67%) was the most abundant ASV in Docking Point B. In Idah Axis Confluence, hgcl clade (65.66%) was the most prevalent ASV, whereas Holophaga (42.86%) was the most common ASV in Idah Axis Midstream. Genera analysis also revealed that 12.9% of the total ASVs were discovered across all sample sites. Among these were pathogenic bacteria, reducers, and degraders of domestic and animal wastes. Observed results provide evidence that sampled sites of Idah River are contaminated, most likely through constant human activities and thus, could have an impact on resident fishes as well. This study, therefore, agrees with a previous report from the river, which used standard microbial procedures. However, next-generation se-

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Section: Discussionmentioning

confidence: 99%

Microbiome Diversity Analysis of the Bacterial Community in Idah River, Kogi State, Nigeria

Adedire¹,

Jimoh²,

Kashim-Bello³

et al. 2022

AiM

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“…Magnetospirillum gryphiswaldense (MSR1) was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) Germany. Magnetospirillum RJS2 (KJ570852), RJS5 (KM289194), RJS6 (KT266803), and RJS7 (KT693285) were isolated and maintained in Magnetospirillum growth medium (MSGM) and oxygensulfide gradient medium (OSGM) using Hungate anaerobic technique (18)(19)(20)(21). The strains were subcultured anaerobically in minimal media MS-1.…”

Section: Strains and Culturing In Minimal Mediummentioning

confidence: 99%

“…Recently, magnetotactic bacteria (MTB) have emerged as a promising candidate for biosorption of metals (9)(10)(11). MTB are freshwater microaerophiles that consist of a membranous intracellular structure called magnetosomes formed during the biomineralization process (12). MTB's can accumulate iron by compartmentalization process and utilize it as a component for its metabolism purpose (13).…”

Section: Introductionmentioning

confidence: 99%

Heavy Metal Removal by Magnetospirillum Strains and Its Possible Use in the Treatment of Tannery Effluents

Sannigrahi¹,

Suthindhiran²

2022

ECS Trans.

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Heavy metals are a threat to the sustainable environmental condition causing ecological imbalance. MTBs utilize iron via a metal transport system known as biomineralization Therefore, the strains MSR-1, RJS2, RJS5, RJS6, and RJS7 were exposed to (1,10 ppm) Cd, Mn, Pb, Ni, Zn, Cr, and Co as a sole electron donor, and time/days for efficient biosorption were optimized. The AAS analysis revealed that the RJS2 strain showed maximum biosorption (1 ppm) of Pb (96%). The strains MSR-1, RJS5, RJS6, and RJS7 produced biosorption of all metals. Biosorption by MSR-1 was more at 10 ppm. However, the RJS5 strain displayed better sorption properties against Pb (93.42%) at 50 ppm. Further exposure of strains against metal biosorption from tannery effluents yielded RJS6 [nickel (88%) and zinc (81%)] and RJS2 [manganese (82%) and cobalt (96%)] as potent strains. The heavy metal removal in MTBs can be further explored on an industrial scale.

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“…Magnetospirillum gryphiswaldense (MSR1) was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) Germany. Magnetospirillum RJS2 (KJ570852), Magnetospirillum RJS5 (KM289194), Magnetospirillum RJS6 (KT266803), and Magnetospirillum RJS7 (KT693285) were isolated from the southern coast of India and maintained in Marine Biotechnology and Bioproducts laboratory, Vellore Institute of Technology, Vellore, India (Jacob et al, 2016). MSR-1, RJS2, and RJS-5 were cultured in Magnetospirillum growth medium (MSGM) (Blakemore et al, 1979) and RJS6, RJS7 were cultured in oxygen-sul de gradient medium (Schuler et al, 1999)…”

Section: Strainsmentioning

confidence: 99%

Heavy Metal Removal by Magnetospirillum Strains and its Possible Use in the Treatment of Tannery Effluents

Sannigrahi

Suthindhiran

2021

Preprint

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Heavy metals pose a serious threat to the environment and their continuous accumulation cause ecological imbalance. Biosorption of heavy metals by bacteria is a conventional process. However, the meagre performance of bacterial biomass in situ limits their applications. Magnetotactic bacteria are microaerophilic organism that possesses an active metal transport system for the biomineralization of iron oxide particles. On this basis, this work investigates the ability of chosen Magnetospirillum strains, viz, MSR-1 RJS2, RJS5, RJS6, and RJS7 to uptake the heavy metals through biomineralization. Metals such as cadmium, lead, zinc, manganese, nickel, chromium, and cobalt were supplemented independently (1 ppm and 10 ppm) in previously optimized MS1 media as a sole electron donor and its utilization by the bacteria were determined by Atomic Absorption Spectroscopy (AAS). Further, the optimal time/days and metal concentration for efficient biosorption were optimized. All the strains were grown in presence of metals and the growth pattern was found to be unaltered due to metal concentration. The AAS analysis revealed metal biosorption (1 ppm) by all five strains. RJS5 strain utilized all the metals viz, cadmium (95.8%), manganese (39.3%), lead (58%), nickel (57%), zinc (55%), chromium (27.5%) and cobalt (78%). Similarly, RJS2 strain showed metal biosorption in cadmium (26.4%), manganese (28%), lead (96%), nickel (30%), zinc (9.2%), chromium (51%) and cobalt (48%). The strain MSR-1 displayed biosorption of five metals- chromium (100%), cadmium (55%), manganese (12%), cobalt (40%) and nickel (4%). Both RJS6 and RJS7 displayed significant biosorption of six metals˗ cadmium (52%), manganese (17.2%), nickel (10%), zinc (34%), chromium (100%) and cobalt (59%) and cadmium (24%), manganese (22%), nickel (7.8%), zinc (40%), chromium (69%) and cobalt (28%) respectively. Metal biosorption in MSR-1 was higher in 10 ppm than 1 ppm concentration. Moreover, metals – lead and nickel biosorption was more evident in 10 ppm concentration by MTBs. RJS5 being the effective strain was exposed to a high concentration of lead, cadmium, and zinc. AAS analysis revealed the biosorption of lead (93.42%) at a 50 ppm concentration. The strains were further exposed for metal biosorption from tannery effluents. RJS6 showed higher biosorption of metals compared to other strains with nickel (88%) and zinc (81%). RJS2 displayed significant biosorption of manganese (82%) and cobalt (96%). The other three strains MSR-1, RJS5, and RJS7 showed moderate biosorption of metals from tannery effluent. The MTB strains showed biosorption against all the metals from tannery effluent. The ability of these strains to remove heavy metals from industrial waste can be further explored for a clean environment.

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Diversity of <i>Magnetospirillum</i> Sp. from the Southern Coast of India

Cited by 6 publications

References 28 publications

Microbiome Diversity Analysis of the Bacterial Community in Idah River, Kogi State, Nigeria

Microbiome Diversity Analysis of the Bacterial Community in Idah River, Kogi State, Nigeria

Heavy Metal Removal by Magnetospirillum Strains and Its Possible Use in the Treatment of Tannery Effluents

Heavy Metal Removal by Magnetospirillum Strains and its Possible Use in the Treatment of Tannery Effluents

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