Diversity of lactic acid bacteria in <i>sian‐sianzih</i> (fermented clams), a traditional fermented food in Taiwan

Chen, Yisheng; Wu, Heng; Li, Ya-han; Leong, Kun-hon; Pua, Xiao-hui; Weng, Ming-kai; Yanagida, Fujitoshi

doi:10.1002/jsfa.4578

Cited by 9 publications

(7 citation statements)

References 29 publications

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“…Besides L. garvieae E‐9 and E‐10, another three L. garvieae strains, which were previously isolated from wild clams (Chen et al . ), fermented clams (Chen et al . ) and fermented ginger (Chang et al .…”

Section: Resultsmentioning

confidence: 99%

“…), fermented clams (Chen et al . ) and fermented ginger (Chang et al . ), were also used as indicator strains.…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, the PPBS from B3-22 showed inhibitory activity against at least 22 indicator strains, indicating a broad antibacterial spectrum. Besides L. garvieae E-9 and E-10, another three L. garvieae strains, which were previously isolated from wild clams (Chen et al 2012), fermented clams (Chen et al 2012) and fermented ginger (Chang et al 2011), were also used as indicator strains. Obvious inhibitory activity against all tested L. garvieae strains was observed ( Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…Isolation was mainly performed following the procedures described by Chen et al (2012). Instead of distilled water, artificial seawater (Tetra Marine Salt Pro, Tetra, Germany) was used for preparing the isolation medium.…”

Section: Isolation Of Lactic Acid Bacteriamentioning

confidence: 99%

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Screening and characterization of LAB-produced bacteriocin-like substances from the intestine of grey mullet (Mugil cephalus L.) as potential biocontrol agents in aquaculture

Chen

Pan

et al. 2012

J Appl Microbiol

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Aim: To isolate and characterize bacteriocin-like inhibitory substance (BLIS)-producing lactic acid bacteria from the intestine of grey mullet. Methods and Results: Inhibitory activity against at least one or more indicator strains was observed in one Enterococcus thailandicus, one Enterococcus faecium and two Lactococcus garvieae strains. Enterococcus faecium B3-8 and Ent. thailandicus B3-22 showed the greatest inhibitory activities against Listeria monocytogenes ATCC 19111 and were therefore further characterized. The results suggested that the inhibitory substances from the two strains showed similar characteristics with respect to sensitivity to heat and proteolytic enzymes. BLIS from Ent. thailandicus B3-22 was characterized by a broader inhibitory spectrum than that from Ent. faecium B3-8. SDS-PAGE revealed that the molecular size of partially purified BLISs from Ent. faecium B3-8 and Ent. thailandicus B3-22 was c. 5 and 3 kDa, respectively. The molecular mass of purified bacteriocin from Ent. thailandicus B3-22 was further determined to be 6319 Da by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The results indicated that BLIS from Ent. thailandicus B3-22 can effectively inhibit the growth of all tested L. garvieae strains. Conclusions: The findings obtained in this study suggest the potential use of Ent. thailandicus B3-22 as a biocontrol agent against pathogenic L. garvieae in the aquaculture. Significance and Impact of the Study: This is the first report describing the characteristics of BLIS from Ent. thailandicus that showed potential for use as a biocontrol agent in the aquaculture.

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Section: Resultsmentioning

confidence: 99%

“…), fermented clams (Chen et al . ) and fermented ginger (Chang et al . ), were also used as indicator strains.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Isolation Of Lactic Acid Bacteriamentioning

confidence: 99%

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Screening and characterization of LAB-produced bacteriocin-like substances from the intestine of grey mullet (Mugil cephalus L.) as potential biocontrol agents in aquaculture

Chen

Pan

et al. 2012

J Appl Microbiol

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“…In a previous study, we reported that W. hellenica 4-7, isolated from fermented clams (sian-sianzih), is capable of producing a bacteriocin-like inhibitory substance (BLIS) (Chen et al 2012). However, the primary structure and biochemical characteristics of the BLIS from W. hellenica 4-7 have not yet been discussed.…”

Section: Introductionmentioning

confidence: 99%

Weissellicin L, a novel bacteriocin from sian-sianzih -isolated Weissella hellenica 4-7

et al. 2013

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Aim: To identify and characterize novel bacteriocins from Weissella hellenica 4-7. Methods and Results: Weissella hellenica 4-7, isolated from the traditional Taiwanese fermented food sian-sianzih (fermented clams), was previously found to produce a bacteriocin active against Listeria monocytogenes and some other Gram-positive bacteria. Bacteriocin activity decreased slightly after autoclaving (121°C for 15 min), but was inactivated by protease K and trypsin. Mass spectrometry analysis revealed the bacteriocin mass to be approximately 3205Á6 Da. N-terminal amino acid sequencing yielded a partial sequence, NH 2 -KGFLSWASKATSWLVGP, by Edman degradation. The obtained partial sequence showed high homology with leucocin B-TA33a; however, at least two different residues were observed. No identical peptide or protein was found, and this peptide was therefore considered to be a novel bacteriocin produced by W. hellenica 4-7 and termed weissellicin L. Conclusions: The findings obtained in the current study suggest a novel bacteriocin produced by W. hellenica 4-7. Significance and Impact of the Study: Bacteriocins from Weissella remain rare, and this study is the second report of a bacteriocin produced by W. hellenica.

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Isolation and characterization of lactic acid bacteria from yan-taozih (pickled peaches) in Taiwan

Chen

Pan

et al. 2012

Ann Microbiol

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Diversity of lactic acid bacteria in sian‐sianzih (fermented clams), a traditional fermented food in Taiwan

Abstract: The results demonstrate that various LAB species are distributed in sian-sianzih and have a great effect on the flavor of sian-sianzih.

Cited by 9 publications

References 29 publications

Screening and characterization of LAB-produced bacteriocin-like substances from the intestine of grey mullet (Mugil cephalus L.) as potential biocontrol agents in aquaculture

Screening and characterization of LAB-produced bacteriocin-like substances from the intestine of grey mullet (Mugil cephalus L.) as potential biocontrol agents in aquaculture

Weissellicin L, a novel bacteriocin from sian-sianzih -isolated Weissella hellenica 4-7

Isolation and characterization of lactic acid bacteria from yan-taozih (pickled peaches) in Taiwan

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