Diversity of Lactic Acid Bacteria Associated with Fish and the Fish Farm Environment, Established by Amplified rRNA Gene Restriction Analysis

Michel, Christian; Pelletier, Claire; Boussaha, Mekki; Douet, Diane-Gaëlle; Lautraite, Armand; Tailliez, Patrick

doi:10.1128/aem.01852-06

Cited by 66 publications

(49 citation statements)

References 44 publications

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“…Some studies have examined genotypic heterogeneity amongst Carnobacterium spp. and other closely related bacteria recovered primarily from food products using the 16S-23S rDNA intergenic spacer region (Kabadjova et al, 2002;Chenoll et al, 2003;Rachman et al, 2004), while Michel et al (2007) examined inter-and intra-species variability in a number of lactic acid bacteria recovered from fish according to amplified rDNA gene restriction analysis and found evidence for intra-species variability in C. maltaromaticum. However, we are unaware of any studies that have examined the genotypic heterogeneity of C. maltaromaticum isolates recovered from fish using 16S rDNA sequence analysis.…”

Section: Biochemical Testmentioning

confidence: 99%

Carnobacterium maltaromaticum infections in feral Oncorhynchus spp. (Family Salmonidae) in Michigan

Loch

Kumar

et al. 2011

J Microbiol.

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Members of the genus Oncorhynchus were introduced from the Pacific Northwest to the Laurentian Great Lakes basin and now constitute one of its most commercially and ecologically valuable fisheries. Recently, infections by a group of Gram-positive atypical lactobacilli belonging to the genus Carnobacterium have been detected in feral and captive Oncorhynchus spp. broodstock, some of which were associated with mortalities. Out of 1564 rainbow and steelhead trout (O. mykiss), coho salmon (O. kisutch), and Chinook salmon (O. tshawytscha) that were bacteriologically examined, 57 Carnobacterium spp. isolates were recovered from the kidneys, spleen, swimbladder, and/or external ulcerations of 51 infected fish. Phenotypic and biochemical characterization, as well as partial 16S rDNA sequencing and phylogenetic analyses of 30 representative isolates identified 29 as Carnobacterium maltaromaticum and 1 as C. divergens, though some phenotypic and genotypic heterogeneity was observed. Infections with C. maltaromaticum were associated with signitures typical of pseudokidney disease, but on occasion were also observed in fish displaying the gross and histopathological changes characteristic of nephrocalcinosis. While C. maltaromaticum infections were found to be widespread in both feral and farmed spawning populations of Oncorhynchus spp. residing within the Great Lakes basin, infection prevalence varied significantly according to fish species and strain, gender, and across time, but not by sampling location according to logistic regression analysis. The findings of this study further underscore the presence of phenotypic variations among Carnobacterium maltaromaticum strains that necessitate genotypic analysis to achieve definitive identification.

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Section: Biochemical Testmentioning

confidence: 99%

Carnobacterium maltaromaticum infections in feral Oncorhynchus spp. (Family Salmonidae) in Michigan

Loch

Kumar

et al. 2011

J Microbiol.

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“…7 and 8). Over the last 60 years, a marked change has occurred in the habitat of S. agalactiae, from predominantly bovine infections to several ecological niches in the environment, animals, and humans, with each colonizing population displaying specific features with respect to the various S. agalactiae lineages (23,29,39,41,50). As shown for other species (38), such changes in habitat expose bacteria to a wide range of environmental and nutritive constraints, imposing stressful conditions that lead to the induction of mutation and contribute to adaptive evolution.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Lytic Activities of Streptococcus agalactiae Bacteriophages and Determination of Lysogenic-Strain Features

et al. 2009

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The application of mitomycin C induction to 114 genetically diverse Streptococcus agalactiae strains generated 36 phage suspensions. On electron microscopy of the phage suspensions, it was possible to assign the phages to the Siphoviridae family, with three different morphotypes (A, B, and C). Phage genetic diversity was evaluated by a PCR-based multilocus typing method targeting key modules located in the packaging, structural, host lysis, lysogeny, replication, and transcriptional regulation clusters and in the integrase genes and by DNA digestion with EcoRI, HindIII, and ClaI. Thirty-three phages clustering in six distantly related molecular phage groups (I to VI) were identified. Each molecular group was morphotype specific except for morphotype A phages, which were found in five of the six phage groups. The various phage groups defined on the basis of molecular group and morphotype had specific lytic activities, suggesting that each recognized particular host cell targets and had particular lytic mechanisms. Comparison of the characteristics of lysogenic and propagating strains showed no difference in the serotype or clonal complex (CC) identified by multilocus sequence typing. However, all the lysogenic CC17 and CC19 strains presented catabolic losses due to a lack of catabolic decay of DL-alpha-glycerol-phosphate substrates (CC17) and of alpha-D-glucose-1-phosphate (CC19). Moreover, the phages from CC17 lysogenic strains displayed lytic replication in bacterial hosts from all S. agalactiae phylogenetic lineages other than CC23, whereas phages obtained from non-CC17 lysogenic strains lysed bacteria of similar evolutionary origin. Our findings suggest that the adaptive evolution of S. agalactiae exposed the bacteria of this species to various phage-mediated horizontal gene transfers, which may have affected the fitness of the more virulent clones.The Lancefield group B Streptococcus, Streptococcus agalactiae-a major cause of neonatal infections-has increasingly been reported as a common pathogen in nonpregnant adults since the 1970s (40). The proportion of neonatal infections caused by serotype III multilocus sequence type 17 (ST-17) strains is higher than would be expected on the basis of the proportion of women and infants colonized by ST-17 strains in control populations (3,5,24,26,30,33). The major clonal complexes (CC) 1, 12, 17, 19, and 23 have been associated with infections in adults (4, 5, 14, 21). The presence in the S. agalactiae genome of particular insertion sequences, a group II intron, and prophage DNA fragments (17,36,49) suggests that horizontal genetic transfer may play an important role in genome diversification and the emergence of virulent clones in S. agalactiae.Temperate phages affect bacterial fitness by modifying anchor points for genome rearrangements, by disrupting genes, by protecting against lytic infection, by lysing competing strains through prophage induction, and by introducing new fitness factors (8, 18). Little is currently known about these phages. S. agalactiae phages we...

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“…Both L. lactis and L. garvieae are also associated with fresh and marine water in tropical areas (Michel et al 2007). In addition to their association with fish farm environments, they are also sometimes isolated from human and other mammalian clinical cases (Michel et al 2007).…”

Section: Microbiota Of Frozen Pangasius Products Marketed In Belgiummentioning

confidence: 99%

Microbiota of frozen Vietnamese catfish (Pangasius hypophthalmus) marketed in Belgium

et al. 2016

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Background: Vietnamese catfish (Pangasius hypophthalmus) is highly appreciated in many European countries, the U.S., Canada, Japan etc. This paper presents an overview of the microbiota of frozen Vietnamese catfish products marketed in Belgium. Samples of Pangasius steaks, portions and fillets from six brands were collected from supermarkets located in Ghent, Belgium. Results: The total psychrotrophic and mesophilic aerobic counts of the samples evaluated from each brand did not differ significantly (p > 0.05) and ranged from 3.8-5.2 log CFU/g and 3.8-4.8 log CFU/g, respectively. Lactic acid bacteria counts varied from 2.2 to 4.1 log CFU/g while the counts of presumptive Enterobacteriaceae ranged from 1.6 to 3.8 log CFU/g. A total of 132 isolates were collected from the plates used to enumerate the microbial parameters mentioned above. Fourteen different genera and 18 different species were identified by means of 16S rRNA gene sequencing. The most prevalent genera of Lactococcus (31.2 %), Staphylococcus (11.7 %), Serratia (10.4 %), Acinetobacter (9.1 %), Enterococcus (7.8 %) and Pseudomonas spp. (6.5 %) were identified by means of 16S rRNA gene sequencing. Conclusion:The results obtained provide an overview of the dominant microbiota on frozen Pangasius which is useful for the development of appropriate preservation techniques for thawed Pangasius products.

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Diversity of Lactic Acid Bacteria Associated with Fish and the Fish Farm Environment, Established by Amplified rRNA Gene Restriction Analysis

Cited by 66 publications

References 44 publications

Carnobacterium maltaromaticum infections in feral Oncorhynchus spp. (Family Salmonidae) in Michigan

Carnobacterium maltaromaticum infections in feral Oncorhynchus spp. (Family Salmonidae) in Michigan

Molecular Characterization and Lytic Activities of Streptococcus agalactiae Bacteriophages and Determination of Lysogenic-Strain Features

Microbiota of frozen Vietnamese catfish (Pangasius hypophthalmus) marketed in Belgium

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