Diversity of <i>Borrelia burgdorferi</i> Sensu Lato in Russian Far East

Mediannikov, Oleg; Иванов, Л. И.; Zdanovskaya, Nina I.; Vorobyova, Rimma; Sidelnikov, Yuri; Fournier, Pierre‐Edouard; Тарасевич, И. В.; Raoult, Didier

doi:10.1111/j.1348-0421.2005.tb03717.x

Cited by 14 publications

(9 citation statements)

References 22 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Borrelia burgdorferi sensu stricto remains the only human pathogen in the United States, while three species ( B. burgdorferi sensu stricto , B. afzelii and B. garinii ) are aetiological agents in Europe, and B. afzelii is the predominated cause of skin lesions . In a previous study, B. afzelii and B. garinii were detected from ticks, rodents and patients in far east Russia and Korea, which is geographically close to Mudanjiang City . In this study, we found three genospecies of B. burgdorferi ( B. garinii , B. afzelii and B. valaisiana ‐related).…”

Section: Discussionmentioning

confidence: 63%

Lyme borreliosis caused by diverse genospecies of Borrelia burgdorferi sensu lato in northeastern China

Jia

Jiang

et al. 2014

Clinical Microbiology and Infection

View full text Add to dashboard Cite

The variety of Borrelia burgdorferi sensu lato (B. burgdorferi) genospecies leads to distinction in clinical manifestations of Lyme borreliosis (LB). There are reports of LB clinical characteristics in China, where the B. burgdorferi genospecies in ticks and animal hosts are different from those in Europe and North America. During May to September in 2010 and 2011, all patients who had erythema migrans (EM, more than 5 cm in diameter) after a recent tick-bite, and sought medical care at Mudanjiang Forestry Central Hospital, Heilongjiang Province of northeastern China, were enrolled in the study. Specific PCR was used to determine the B. burgdorferi genospecies in the disseminated patients. Of 265 EM patients, B. burgdorferi DNA was detected in blood specimens from 15 of 55 disseminated patients. Sequence analyses of 5S-23S rRNA, flagellin, ospC, 16S rRNA and ospA genes revealed that 11 patients were infected with Borrelia garinii, three with Borrelia afzelii and one with Borrelia valaisiana-related genospecies. Among 15 patients, 40%, 13.3% and 13.3% manifested pruritus, pain and ulceration, respectively. Systemic symptoms, arthralgia or a swollen joint and lymphadenopathy were observed in 26.7%, 13.3% and 6.7% patients, respectively. In northeastern China, three genospecies of LB patients were detected. The B. burgdorferi genospecies identified in this study was predominantly B. garinii. A case infected with B. valaisiana-related genospecies was reported for the first time.

show abstract

Section: Discussionmentioning

confidence: 63%

Lyme borreliosis caused by diverse genospecies of Borrelia burgdorferi sensu lato in northeastern China

Jia

Jiang

et al. 2014

Clinical Microbiology and Infection

View full text Add to dashboard Cite

show abstract

“…The presence of multiple strains in an area of Lyme disease endemicity could also complicate diagnosis (2,13,19,25,26,31). An antigen that contains epitopes which remain conserved and expressed across the B. burgdorferi sensu lato population is of extraordinary importance to diagnostic sensitivity and accuracy.…”

mentioning

confidence: 99%

Borrelia burgdorferi Spirochetes That Harbor Only a Portion of the lp28-1 Plasmid Elicit Antibody Responses Detectable with the C ₆ Test for Lyme Disease

Embers

Wormser

Schwartz

et al. 2007

Clin Vaccine Immunol

View full text Add to dashboard Cite

Detection of antibody to C 6 , a peptide that reproduces the sequence of the sixth invariable region within the central domain of the VlsE protein of Borrelia burgdorferi, is used currently for the serologic diagnosis of Lyme disease in humans. B. burgdorferi isolates taken from infected humans can be categorized into specific genetic subtypes (designated RST1, -2, and -3) by restriction fragment length polymorphisms in the 16S to 23S rRNA spacer sequence. Many of these, usually categorized as RST2, retain only segments of the linear plasmid lp28-1, which encodes VlsE. The VlsE genetic region is retained, but altered expression of this molecule could affect diagnosis by the C 6 enzyme-linked immunosorbent assay (ELISA). Serum samples from patients infected with each of the three genotypes and from mice infected with three RST2 isolates were tested with the C 6 ELISA. Such isolates elicited marked C 6 responses in infected mice. The sensitivity of C 6 antibody detection in patients infected with RST2 spirochetes was statistically indistinguishable from detection of RST1 and RST3 infections. These findings demonstrate that diagnosis by C 6 ELISA remains effective for infection with all B. burgdorferi genotypes, including those with incomplete lp28-1 plasmids.The clinical progression of Lyme disease, a tick-borne illness that is caused by the spirochete Borrelia burgdorferi, is divided into early localized, early disseminated, and late stages. During the early localized phase, the disease usually manifests by a characteristic skin lesion, erythema migrans (EM). Several days or weeks later, the spirochetes may spread hematogenously and patients may develop early disseminated disease with dermatologic, cardiac, neurologic, and/or rheumatologic involvement. During the early disseminated phase, some patients exhibit dermatologic signs that appear as multiple EM (24,27,29,33). Late disease presents mainly with arthritis or neurologic manifestations (28).Evidence has been put forward to suggest that a major determinant of the risk of B. burgdorferi dissemination in Lyme disease patients with EM is the genotype of the infecting spirochetal strain. Three genotypes have been identified on the basis of restriction fragment length polymorphisms in the rRNA spacer region and have been designated RST1, -2, and -3 (16). Patients infected with RST1 spirochetes had the highest proportion of blood culture positivity (43%), those infected with RST2 spirochetes were intermediate (20%), and those with RST3 had the lowest proportion (3%). In general, persons with an RST1 infection had more symptoms and symptoms of greater severity than those with either RST2 or RST3 infection (32). Mice that were inoculated with RST1 organisms were significantly more likely to yield cultivable spirochetes from different organs and showed a significantly higher prevalence of both carditis and arthritis than did animals inoculated with RST3 spirochetes (30); RST2 isolates were not assessed. When the plasmid contents of the different human infectious type...

show abstract

“…In our study, DNA of B. garinii NT29 group was detected in Lyme borreliosis cases with neurological disorders as well as in mixed TBE/LB infection with EM (Fomenko et al, 2006). Previously, B. garinii NT29 was isolated from the skin biopsy of a patient with EM in Khabarovsk region (Medianikov et al, 2005). We detected B. garinii in 74% of all PCR-positive blood samples of patients with EM, B. afzelii in 23%, and both species in 5% (n ¼ 40) ( Table 1).…”

Section: Discussionmentioning

confidence: 68%

“…B. garinii NT29 to 20047 group ratio was about 2:1. B. garinii was also shown to be the predominant species in I. persulcatus in Khabarovsk Territory and in the Da Xing-An Mountains Forest areas of China (Medianikov et al, 2005, Chu et al, 2006. B. garinii NT29 group dominates in I. persulcatus in both Novosibirsk region and Da Xing-An Mountains Forest areas of China.…”

Section: Article In Pressmentioning

confidence: 96%

Diversity of Borrelia burgdorferi sensu lato in natural foci of Novosibirsk region

Фоменко¹,

Ливанова²,

Chernousova³

2008

International Journal of Medical Microbiology

View full text Add to dashboard Cite

Ixodes persulcatus ticks, wild small mammals, and infected people in Novosibirsk region of Russia were investigated to analyze the local distribution and diversity of Borrelia burgdorferi sensu lato. The molecular typing was based on the partial sequencing of the 16S rRNA gene and the 5S-23S rRNA intergenic spacer region. The analysis of our data revealed the presence of two Borrelia species, B. garinii and B. afzelii. B. garinii DNA was found much more often, namely in 74% of all PCR-positive human blood samples, in 72% of all samples from small mammals (including blood and tissues), and in 76% of Borrelia-positive unfed adult I. persulcatus. B. afzelii DNA was found in 21% of the PCR-positive human blood samples, in 20% of the PCR-positive samples from the small mammals (including blood and tissues) and in 19% of the PCR-positive ticks. Mixed infection with B. garinii and B. afzelii was observed in both clinical and environmental samples. Interestingly, four samples isolated from I. persulcatus were closely related to the relapsing fever species B. miyamotoi.

show abstract

Diversity of Borrelia burgdorferi Sensu Lato in Russian Far East

Cited by 14 publications

References 22 publications

Lyme borreliosis caused by diverse genospecies of Borrelia burgdorferi sensu lato in northeastern China

Lyme borreliosis caused by diverse genospecies of Borrelia burgdorferi sensu lato in northeastern China

Borrelia burgdorferi Spirochetes That Harbor Only a Portion of the lp28-1 Plasmid Elicit Antibody Responses Detectable with the C ₆ Test for Lyme Disease

Diversity of Borrelia burgdorferi sensu lato in natural foci of Novosibirsk region

Contact Info

Product

Resources

About

Diversity of Borrelia burgdorferi Sensu Lato in Russian Far East

Cited by 14 publications

References 22 publications

Lyme borreliosis caused by diverse genospecies of Borrelia burgdorferi sensu lato in northeastern China

Lyme borreliosis caused by diverse genospecies of Borrelia burgdorferi sensu lato in northeastern China

Borrelia burgdorferi Spirochetes That Harbor Only a Portion of the lp28-1 Plasmid Elicit Antibody Responses Detectable with the C 6 Test for Lyme Disease

Diversity of Borrelia burgdorferi sensu lato in natural foci of Novosibirsk region

Contact Info

Product

Resources

About

Borrelia burgdorferi Spirochetes That Harbor Only a Portion of the lp28-1 Plasmid Elicit Antibody Responses Detectable with the C ₆ Test for Lyme Disease