Diversity ofBacillus-like bacterial community in the sediments of the Bamenwan mangrove wetland in Hainan, China

Abstract

Members of the genus Bacillus and related spore-forming genera are ubiquitous. However, Bacillus-like species isolated from marine sediments have attracted less interest than their terrestrial relatives. Here, we investigated the diversity of Bacillus-like bacterial communities in the sediments of the Bamenwan mangrove wetland in Hainan, China, using culture-dependent and culture-independent methods, and present the first report on this subject. We also discovered some potential novel species from the sediment samples. Four families, Bacillaceae (58%), Paenibacillaceae (22%), Alicyclobacillaceae (15%), and Planococcaceae (5%), and 9 genera, Bacillus (42%), Paenibacillus (16%), Halobacillus (13%), Alicyclobacillus (11%), Rummeliibacillus (5%), Cohnella (5%), Tumebacillus (4%), Pontibacillus (3%), and Aneurinibacillus (2%), were identified by pyrosequencing. In contrast, only 4 genera, Bacillus (57%), Paenibacillus (23%), Halobacillus (14%), and Virgibacillus (6%), were detected by the culture-dependent method. In the 16S rDNA sequencing analysis, the isolates HB12036 and HB12037 were closest to Bacillus okuhidensis Kh10-101 and Paenibacillus xylanilyticus XIL14 with similarities of 94.8% and 95.9%, respectively, indicating that these were novel species. Bacillus sp. HB12035 and HB12040 exhibited antimicrobial activity against Staphylococcus aureus ATCC 25923, and Bacillus sp. HB12033 exhibited antimicrobial activity against Ustilago scitaminea Syd.

Identification of Novel Microsatellite Markers to Assess the Population Structure and Genetic Differentiation of Ustilago hordei Causing Covered Smut of Barley

“…The results of the present study revealed low but significant genetic differentiation in U. hordei isolates with a similarity coefficient of over 60%. Parallel to these findings, Xu et al (2004) also documented the phenomenon of diverse geographical origin of U. scitaminea population with small values of dissimilarity coefficients (Owati et al, 2019). The low level of genetic differentiation and formation of two distinct sub-groups within U. hordei isolates of PZ reflected an extreme event of genetic discrimination that happened at a small scale, probably due to migration.…”

The Diversity and Geographic Distribution of Cultivable Bacillus-Like Bacteria Across Black Soils of Northeast China

“…The traditional way to analyze the diversity of Bacillus -like bacteria is based on bacterial strain isolation and 16S rRNA gene identification (Maughan and Van der Auwera, 2011; Dheemana et al, 2017; Panosyan et al, 2018). Recently, the use of HTS surveying of Bacillus -like bacteria directly in soils and combining it with a one-by-one isolation method was also reported (Liu et al, 2017; Mukhtar et al, 2018). Those studies showed that more Bacillus -like bacterial genera were detected by HTS than by the culture-dependent method, which indicated that HTS was a more effective way to discover specific microbial diversity (Li et al, 2015).…”

“…Given that most of Bacillus -like bacteria are cultivable (Seldin et al, 1998; McSpadden Gardener, 2014), and most of studies investigating the distribution of Bacillus -like bacteria in soils are based on the isolation method, and the isolated strains were identified by 16S rRNA gene sequencing one by one (Liu et al, 2016, 2017; Mukhtar et al, 2018). This protocol is a laborious and time-consuming, which restricts us to investigate the diversity of Bacillus -like bacteria with high efficiency.…”

Diversity of Bacillus-like bacterial community in the rhizospheric and non-rhizospheric soil of halophytes (Salsola stocksii and Atriplex amnicola), and characterization of osmoregulatory genes in halophilic Bacilli

“…In saline environments, members of the phylum Firmicutes, e.g., Bacillus, Virgibacillus, Halobacillus, Oceanobacillus, Paenibacillus and Brevibacillus have found to be more abundant as compared to other bacteria (Liszka et al 2012). Halophilic Bacilli have a wide range of applications in bioenzyme production, biodefense, biofuel production and bioremediation of organic toxic compounds (Lundberg et al 2012;Liu et al 2017). These Bacilli are a good source of novel enzymes that function under salt stress conditions, such as proteases, xylanases, cellulases and amylases with polyextremophilic properties (Taprig et al 2013).…”