Diversity of Hot Environments and Thermophilic Microbes

Mehta, Deepika; Satyanarayana, T.

doi:10.1007/978-94-007-5899-5_1

Cited by 30 publications

(20 citation statements)

References 415 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, it is important to bear in mind that the compost-borne fungi, which were present in mesh bags at both horizons and treatments, may or may not be adapted to the new conditions imposed in the environment. For instance, the saprotrophic fungi Myceliophthora heterothallica and Aspergillus fumigatus are compost-borne fungi that grow well under thermophilic conditions (Mehta and Satyanarayana 2013), but they would not be expected to grow at the low temperatures of the studied forest soils. In addition, some portion of these sequence reads likely arose from DNA extracted from dormant spores rather than active hyphae.…”

Section: Discussionmentioning

confidence: 99%

Chemical changes in organic matter after fungal colonization in a nitrogen fertilized and unfertilized Norway spruce forest

et al. 2017

View full text Add to dashboard Cite

Background and aims Decomposition and transformation of organic matter (OM) in forest soils are conducted by the concomitant action of saprotrophic and mycorrhizal fungi. Here, we examine chemical changes in OM after fungal colonization in nitrogen fertilized and unfertilized soils from a Norway spruce forest. Methods Sand-filled bags amended with composted maize leaves were placed in the forest soil and harvested after 17 months. Infrared and near edge X-ray absorption fine structure spectroscopies were used to study the chemical changes in the OM. Fungal community composition of the bags was also evaluated. Results The proportion of ectomycorrhizal fungi declined in the fertilized plots, but the overall fungal community composition was similar between N treatments. Decomposition of the OM was, independently of the N level or soil horizon, accompanied by an increase of C/N ratio of the mesh-bag content. Moreover, the proportions of carboxylic compounds in the incubated OM increased in the mineral horizon, while heterocyclic-N compounds decreased, especially in unfertilized plots with higher N demand from the trees. Conclusions Our results indicate that more oxidized organic C and less heterocyclic-N proportions in the OM remain after fungal colonization in the mineral layers, and suggest that ectomycorrhizal fungi transfer less heterocyclic-N from the mesh bags to the host trees under high N levels.

show abstract

Section: Discussionmentioning

confidence: 99%

Chemical changes in organic matter after fungal colonization in a nitrogen fertilized and unfertilized Norway spruce forest

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Most Actinobacteria are saprophytic and soil-dwelling organisms, thus they are abundant in soil (Barka et al 2016). Actinobacteria occur in various environments, including extreme environments defined by high temperatures, such as geothermal and volcanic areas, terrestrial hot springs, and geysers (Mehta and Satyanarayana 2013;Shivlata and Satyanarayana 2015). Thermophilic Actinobacteria can grow at relatively high temperatures ranging from 40 to 80 o C (Shivlata and Satyanarayana 2015).…”

Section: Introductionmentioning

confidence: 99%

“…The discovery of a thermostable enzyme produced by Actinobacteria has attracted the interest of researchers and prompted them to explore geothermal regions (e.g. volcanic areas, hydrothermal areas, and geysers) (Mehta and Satyanarayana 2013). Cisolok is one of the geothermal areas in West Java, Indonesia (Purnomo and Pichler 2014), that is still less exploited, and is thus a high-potential area for isolation of novel thermophilic Actinobacteria.…”

Section: Introductionmentioning

confidence: 99%

Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a Geothermal Area in West Java, Indonesia

et al. 2019

View full text Add to dashboard Cite

Abstract. Syafitri WA, Ningsih F, Setyaningsih PP, Rachmania MK, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridzal W. 2019. Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a geothermal area in West Java, Indonesia. Biodiversitas 20: 1929-1938. In this study, we describe the screening for amylolytic activity of 17 thermophilic Actinobacteria isolates obtained from the soil of Cisolok geysers, a geothermal area in West Java, Indonesia. All isolates were screened for amylolytic activity by the starch-agar plate method at various temperatures. The results showed that all of isolates were able to grow at 45oC. The growth abilities of the isolates grown in ISP 1 medium varied at temperatures from 45 to 60oC. Fifteen of the 17 isolates showed amylolytic activity at 45oC, 13 showed such activity at 50oC, and four showed activity at 55oC. Only three isolates, designated SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4, showed growth and amylolytic activity at 60oC. These three isolates were selected for molecular identification. The nearly full-length of 16S rRNA gene sequences data showed that these three isolates have a similarity of 99.93-100% with Actinomadura keratinilytica WCC-2265T and of 98.74-98.91% with A. miaoliensis BC 44T-5T. Phylogenetic tree shows that all three isolates are clustered together in a monophyletic group with the type strain of A. keratinilytica WCC-2265T as their most closely related species, with 100% bootstrap support. Based on sequencing of the 16S rRNA gene, phylogenetic comparison, and phenotypic characterization, the three isolates were identified as A. keratinilytica.

show abstract

“…Although several thermostable enzymes have been recently described and characterized (e.g. [ 2 – 4 ]), thermophiles are still highly unexplored [ 5 ], especially because the majority of prokaryotic diversity cannot be cultured [ 6 ]. There have only been a few attempts to characterize enzymes or microorganisms from Neotropics hot springs (e.g.…”

Section: Introductionmentioning

confidence: 99%

CLAME: a new alignment-based binning algorithm allows the genomic description of a novel Xanthomonadaceae from the Colombian Andes

et al. 2018

View full text Add to dashboard Cite

BackgroundHot spring bacteria have unique biological adaptations to survive the extreme conditions of these environments; these bacteria produce thermostable enzymes that can be used in biotechnological and industrial applications. However, sequencing these bacteria is complex, since it is not possible to culture them. As an alternative, genome shotgun sequencing of whole microbial communities can be used. The problem is that the classification of sequences within a metagenomic dataset is very challenging particularly when they include unknown microorganisms since they lack genomic reference. We failed to recover a bacterium genome from a hot spring metagenome using the available software tools, so we develop a new tool that allowed us to recover most of this genome.ResultsWe present a proteobacteria draft genome reconstructed from a Colombian’s Andes hot spring metagenome. The genome seems to be from a new lineage within the family Rhodanobacteraceae of the class Gammaproteobacteria, closely related to the genus Dokdonella. We were able to generate this genome thanks to CLAME. CLAME, from Spanish “CLAsificador MEtagenomico”, is a tool to group reads in bins. We show that most reads from each bin belong to a single chromosome. CLAME is very effective recovering most of the reads belonging to the predominant species within a metagenome.ConclusionsWe developed a tool that can be used to extract genomes (or parts of them) from a complex metagenome.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5191-y) contains supplementary material, which is available to authorized users.

show abstract

Diversity of Hot Environments and Thermophilic Microbes

Cited by 30 publications

References 415 publications

Chemical changes in organic matter after fungal colonization in a nitrogen fertilized and unfertilized Norway spruce forest

Chemical changes in organic matter after fungal colonization in a nitrogen fertilized and unfertilized Norway spruce forest

Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a Geothermal Area in West Java, Indonesia

CLAME: a new alignment-based binning algorithm allows the genomic description of a novel Xanthomonadaceae from the Colombian Andes

Contact Info

Product

Resources

About