Diversity of Fusarium species associated with malformed inflorescences of Syzygium cordatum

Kvas, Marija; Steenkamp, Emma Theodora; Wingfield, B. D.; Marasas, W. F. O.; Wingfield, M.J.

doi:10.1016/j.sajb.2008.01.072

Cited by 5 publications

(3 citation statements)

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“…Analysing 11 new Illumina genome assemblies of five F. xylarioides strains (Table S1, 5 Figure 1) and six public Fusarium genomes (Table 2), together with 25 published Fusarium genomes (Table S2), assigned protein-encoding genes to 26,000 orthologous groups, of which 3544 had members in all genomes with 1685 single-copy genes. A species tree based on these 3544 orthogroups supported phylogenetic relationships in the Fusarium genus from earlier studies with lower sampling [26,27], Figure 2A). Fusarium xylarioides forms a monophyletic clade within the F. fujikuroi species complex (Figure 2A), a result that is supported by the majority of gene trees (94%) (Figure S1) and by the high average coding sequence identities among F. xylarioides (98%) contrasting with lower similarity with F. phyllophilum (96%), other species of the F. fujikuroi complex (91-92%) and F. oxysporum strains (90%) (Figure 2B).…”

Section: Resultssupporting

confidence: 66%

Horizontal transfers between fungalFusariumspecies contributed to successive outbreaks of coffee wilt disease

Peck,

Llewellyn,

Bennetot

et al. 2023

Preprint

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Outbreaks of fungal disease have devastated plants and animals throughout history. Over the past century, the repeated emergence of coffee wilt disease caused by the fungal pathogenFusarium xylarioidesseverely impacted coffee production across sub-Saharan Africa. To improve the disease management of such pathogens, it is crucial to understand their genetic structure and evolutionary potential. We compared the genomes of 13 historic strains spanning six decades and multiple disease outbreaks to investigate population structure and host specialisation. We foundF. xylarioidescomprises at least four distinct lineages: one host-specific toCoffea arabica, one toC. canephoravar.robusta, and two historic lineages isolated from variousCoffeaspecies. Mapping variation onto a new long-read reference genome showed that host-specificity appears to be acquired through horizontal transfer of effector genes from members of theF. oxysporumspecies complex. This species complex is known to cause wilt disease in over 100 plant species. Multiple transfers into theF. xylarioidespopulations matched to different parts of theF. oxysporummobile pathogenicity chromosome and were enriched in effector genes and transposons. Effector genes in this region and other horizontally transferred carbohydrate-active enzymes important in the breakdown of plant cell walls were shown by transcriptomics to be highly expressed during infection ofC. arabicaby the fungal arabica strains. Widespread sharing of specific transposons betweenF. xylarioidesandF. oxysporum, and the presence of largeStarshipelements, indicate that transposons were involved in horizontal transfers. Our results support the hypothesis that horizontal gene transfers contributed to the repeated emergence of this fungal disease.

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Section: Resultssupporting

confidence: 66%

Horizontal transfers between fungalFusariumspecies contributed to successive outbreaks of coffee wilt disease

Peck,

Llewellyn,

Bennetot

et al. 2023

Preprint

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“…The representatives of F. subglutinans came from the study of Steenkamp et al (2001) . All of the isolates from Syzigium cordatum originated from a previous survey of the diversity of Fusarium species associated with this host in South Africa ( Kvas et al 2008 ; E. Steenkamp, unpublished data).…”

Section: Methodsmentioning

confidence: 99%

Genome-Based Selection and Characterization ofFusarium circinatum-Specific Sequences

Maphosa

Steenkamp

Wingfield

2016

G3 Genes|Genomes|Genetics

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Fusarium circinatum is an important pathogen of pine trees and its management in the commercial forestry environment relies largely on early detection, particularly in seedling nurseries. The fact that the entire genome of this pathogen is available opens new avenues for the development of diagnostic tools for this fungus. In this study we identified open reading frames (ORFs) unique to F. circinatum and determined that they were specific to the pathogen. The ORF identification process involved bioinformatics-based screening of all the putative F. circinatum ORFs against public databases. This was followed by functional characterization of ORFs found to be unique to F. circinatum. We used PCR- and hybridization-based approaches to confirm the presence of selected unique genes in different strains of F. circinatum and their absence from other Fusarium species for which genome sequence data are not yet available. These included species that are closely related to F. circinatum as well as those that are commonly encountered in the forestry environment. Thirty-six ORFs were identified as potentially unique to F. circinatum. Nineteen of these encode proteins with known domains while the other 17 encode proteins of unknown function. The results of our PCR analyses and hybridization assays showed that three of the selected genes were present in all of the strains of F. circinatum tested and absent from the other Fusarium species screened. These data thus indicate that the selected genes are common and unique to F. circinatum. These genes thus could be good candidates for use in rapid, in-the-field diagnostic assays specific to F. circinatum. Our study further demonstrates how genome sequence information can be mined for the identification of new diagnostic markers for the detection of plant pathogens.

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“…Although presumably a disease far back in antiquity, the first recording of one such pathogen occurred in Japan in 1828 with a disease named, bakanae or "Foolish Rice Seedling Disease." (Aoki et al, 2014;Kvas et al, 2009;Stowe & Amakl2, n.d.) Plants infected with this disease rapidly elongate and take on a sickly yellow hue before ultimately falling over and dying. As they weaken, hyphae from the ascomycete fungus Fusarium fujikuroi invade and consume the plant tissue, ultimately developing spores that diffuse readily to neighboring plants in the wet patty conditions or on seeds to be sown in the following season.…”

Section: Conceptual Backgroundmentioning

confidence: 98%

Transcending eons; evolutionary and mechanistic insight into plant Class I Diterpene synthases

Brown

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Diversity of Fusarium species associated with malformed inflorescences of Syzygium cordatum

Cited by 5 publications

References 0 publications

Horizontal transfers between fungalFusariumspecies contributed to successive outbreaks of coffee wilt disease

Horizontal transfers between fungalFusariumspecies contributed to successive outbreaks of coffee wilt disease

Genome-Based Selection and Characterization ofFusarium circinatum-Specific Sequences

Transcending eons; evolutionary and mechanistic insight into plant Class I Diterpene synthases

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