Diversity of foliar endophytic ascomycetes in the endemic Corsican pine forests

Taudière, Adrien; Bellanger, Jean‐Michel; Carcaillet, Christopher; Hugot, Laëtitia; Kjellberg, Finn; Lecanda, Aarón; Lesne, Annick; Moreau, Pierre‐Arthur; Scharmann, Karin; Leidel, Sebastian A.; Richard, Franck

doi:10.1016/j.funeco.2018.07.008

Cited by 15 publications

(17 citation statements)

References 64 publications

(83 reference statements)

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“…Ascomycota and Basidiomycota are the two most abundant fungal taxa in host-microbial communities [ 71 , 72 , 73 ]. In our study, Basidiomycota was more abundant in the needles of healthy trees, whereas Ascomycota had a higher abundance in diseased trees.…”

Section: Discussionmentioning

confidence: 99%

The Impact of Pine Wood Nematode Infection on the Host Fungal Community

Liu

et al. 2021

Microorganisms

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Pine wilt disease (PWD), caused by pinewood nematode (PWN) Bursaphelenchus xylophilus, is globally one of the most destructive diseases of pine forests, especially in China. However, little is known about the effect of PWD on the host microbiome. In this study, the fungal community and functional structures in the needles, roots, and soil of and around Pinus thunbergii naturally infected by PWN were investigated by using high-throughput sequencing coupled with the functional prediction (FUNGuild). The results showed that fungal richness, diversity, and evenness in the needles of diseased trees were significantly lower than those of healthy ones (p < 0.05), whereas no differences were found in the roots and soil. Principal coordinate analysis (PCoA) showed that the fungal community and functional structures significantly differed only in the needles of diseased and healthy trees, but not in the soil and roots. Functionally, the saprotrophs had a higher abundance in the needles of diseased trees, whereas symbiotrophs abundance was higher in the needles of healthy trees (linear discriminant analysis (LDA) > 2.0, p < 0.05). These results indicated that PWN infection primarily affected the fungal community and functional structures in the needles of P. thunbergii, but not the roots and soil.

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Section: Discussionmentioning

confidence: 99%

The Impact of Pine Wood Nematode Infection on the Host Fungal Community

Liu

et al. 2021

Microorganisms

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“…Foliar fungal endophyte assemblages have been shown to vary between the developmental stage of the host (mature vs. seedling (Helander et al, 2011;Koukol et al, 2012;Skaltsas et al, 2019). The core foliar endophytes seem to be the same in seedlings and mature Scots pine (Blumenstein et al, 2020;Bußkamp et al, 2020) but community compositions and species differences are probably related to the age of the host (Taudière et al, 2018). The genesis of the Scots pine endophytic community is poorly known.…”

Section: Pre-colonization Of the Trees With Fungal Communitymentioning

confidence: 99%

Sphaeropsis sapinea and Associated Endophytes in Scots Pine: Interactions and Effect on the Host Under Variable Water Content

Blumenstein

Bußkamp

Langer

et al. 2021

Front. For. Glob. Change

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The ascomycete Sphaeropsis sapinea is the causal agent of the Diplodia Tip Blight disease on pines and other conifer species. This fungus has a symptomless endophytic life stage. Disease symptoms become visible when trees have been weakened by abiotic stress, usually related to warmer temperatures and drought. Currently, this disease is observed regularly in Scots pine (Pinus sylvestris) sites in parts of Europe, such as Germany, increasing dramatically in the last decade. Changes in climatic conditions will gradually increase the damage caused by this fungus, because it is favored by elevated temperature. Thus, host trees with reduced vitality due to climate change-related environmental stress are expected to be more susceptible to an outbreak of Diplodia Tip Blight disease. There is currently no established and effective method to control S. sapinea. This project aims to reveal the nature of the endophyte community of Scots pine. Utilizing the antagonistic core community of endophytes could serve as a novel tool for disease control. Results from this study provide a starting point for new solutions to improve forest health and counter S. sapinea disease outbreaks. We screened potential antagonistic endophytes against S. sapinea and infected Scots pine seedlings with the most common endophytes and S. sapinea alone and combination. The host was stressed by limiting access to water. The antagonism study revealed 13 possible fungi with the ability to inhibit the growth of S. sapinea in vitro, for example Sydowia polyspora. None of the tested co-infected fungi (Desmazierella acicola, Didymellaceae sp., Microsphaeropsis olivacea, Sydowia polyspora, and Truncatella conorum-piceae) showed strong necrosis development in vivo, even when host stress increased due to drought. However, the infection experiment demonstrated that drought conditions enhance the effect of the disease outbreak, triggering S. sapinea to cause more necrosis in the infected twigs.

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“…The species composition and relative abundance of endophytic fungi in the leaves of broadleaf plants can vary widely between developmental stages of the diverse hosts (natural old forests, managed mature forests and seedling stands) [143]. Taudière et al [144] found that young (two-year old) and mature trees (10-15 years) host similar numbers of foliar endophytes with some differences in community compositions and species richness. Similarly, Koukol et al [145] documented differences in fungal endophyte species composition of P. abies needles obtained from diverse host sources (seedlings, mature trees, clonal cuttings, wind-fallen).…”

Section: Aerial Endophytesmentioning

confidence: 99%

Forest Tree Microbiomes and Associated Fungal Endophytes: Functional Roles and Impact on Forest Health

Terhonen

Blumenstein

Kovalchuk

et al. 2019

Forests

159

113

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Terrestrial plants including forest trees are generally known to live in close association with microbial organisms. The inherent features of this close association can be commensalism, parasitism or mutualism. The term “microbiota” has been used to describe this ecological community of plant-associated pathogenic, mutualistic, endophytic and commensal microorganisms. Many of these microbiota inhabiting forest trees could have a potential impact on the health of, and disease progression in, forest biomes. Comparatively, studies on forest tree microbiomes and their roles in mutualism and disease lag far behind parallel work on crop and human microbiome projects. Very recently, our understanding of plant and tree microbiomes has been enriched due to novel technological advances using metabarcoding, metagenomics, metatranscriptomics and metaproteomics approaches. In addition, the availability of massive DNA databases (e.g., NCBI (USA), EMBL (Europe), DDBJ (Japan), UNITE (Estonia)) as well as powerful computational and bioinformatics tools has helped to facilitate data mining by researchers across diverse disciplines. Available data demonstrate that plant phyllosphere bacterial communities are dominated by members of only a few phyla (Proteobacteria, Actinobacteria, Bacteroidetes). In bulk forest soil, the dominant fungal group is Basidiomycota, whereas Ascomycota is the most prevalent group within plant tissues. The current challenge, however, is how to harness and link the acquired knowledge on microbiomes for translational forest management. Among tree-associated microorganisms, endophytic fungal biota are attracting a lot of attention for their beneficial health- and growth-promoting effects, and were preferentially discussed in this review.

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Diversity of foliar endophytic ascomycetes in the endemic Corsican pine forests

Cited by 15 publications

References 64 publications

The Impact of Pine Wood Nematode Infection on the Host Fungal Community

The Impact of Pine Wood Nematode Infection on the Host Fungal Community

Sphaeropsis sapinea and Associated Endophytes in Scots Pine: Interactions and Effect on the Host Under Variable Water Content

Forest Tree Microbiomes and Associated Fungal Endophytes: Functional Roles and Impact on Forest Health

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