Diversity of filamentous and yeast fungi in soil of citrus and grapevine plantations in the Assiut region, Egypt.

Abstract

An extensive survey of soil mycobiota on citrus and grapevine plantations in Sahel-Saleem City, Assiut Governorate, Egypt was carried out using the dilution-plate method and 2 isolation media at 25°C. Sixty-four genera and 195 species of filamentous fungi and 10 genera and 13 species of yeasts were recovered. A higher diversity (number of genera and species) and gross total counts were recovered from citrus than from grapevine soil. The peak of filamentous fungi recovered from both soils was found to be in February. Aspergillus (45 species) was the most dominant genus; A. ochraceus predominated in citrus plantations, while A. niger and A. aculeatus in grapevine. The Penicillium count came second after Aspergillus in citrus (23 species) and after Aspergillus and Fusarium in grapevine (11 species). Penicillium citrinum, P. ochrochloron and P. olsonii were more common in citrus plantations, but they were replaced by P. oxalicum in grapevine soil. Fusarium (19 species) was represented in 88.9-100% of both soils on both media; F. solani predominated in both soils, while F. incarnatum came next in citrus, and F. babinda and F. oxysporum in grapevine. Humicola (3 species) with the dominant H. fuscoatra was recorded in 61.1-83.3% of soil of both plantations, while Talaromyces (with T. purpureogenus followed by T. pinophilus being the most common) was recorded in 83.3-100% on DRBC and 38.9-50% on DYM from the soil of plantations of both crops. Volutella (5 species) was common in citrus but missing from grapevine soil. The present study reveals that hyaline fungi predominated over dark-coloured ones. Yeasts comprised only minor proportions in both soils (maximum 0.5%). They showed their peak in the soil of citrus plantations in April and in grapevine in February. All species were recovered in one or two samples only. Diutina catenulata, Debaryomyces hansenii, Galactomyces (3 species), Hanseniaspora occidentalis, Kluyveromyces marxianus, Meyerozyma caribbica, and Schwanniomyces pseudopolymorphus were encountered in citrus only, while Cryptococcus laurentii, Pichia kudriavzevii, Meyerozyma guilliermondii and Rhodotorula sp. in grapevine only. Physiological and growth characteristics were obtained for most of the recovered yeasts.

Diversity of yeasts and filamentous fungi in mud from hypersaline and freshwater bodies in Egypt.

“…Our previous experience (Abdel-Sater et al 2016, Moubasher et al 2017 revealed that zygomycetes prevailed on most of the isolation media used, therefore the first two media were finally chosen since both contained dichloran, which restricts mucoraceous growth without affecting other species (King et al 1979, Hocking & Pitt 1980. The first medium (a w = 1.0) was used as a general isolation medium (King et al 1979), the second (a w = 0.95) for xerotolerant fungi and the third (a w = 0.89) for xerophilic ones (Gunde-Cimerman et al 2000, Butinar et al 2005.…”

“…Then these bands were eluted and sequenced. Each sample was sequenced in both directions (Abdel-Sater et al 2016). Raw sequences were assembled in contigs with the CLCBio Main Workbench program.…”

Cadmium biosorption potential and kinetic behavior of endophytic Fusarium verticillioides and its green synthesized silver nanoparticles (GSNPs)

“…The genomic DNA of Fusarium isolate was extracted using the cetyltrimethyl ammonium bromide method. Universal internal transcribed spacer (ITS) primers ITS1 and ITS4 were used for the amplification of ~520 bp rDNA fragments using a polymerase chain reaction (PCR) as described by Abdel-Sater et al (2016). The Basic Local Alignment Search Tool of Nucleotides program was used to compare the sequences and to retrieve the homologous nucleotide sequences from the National Center for Biotechnology Information (NCBI) database (www.ncbi.nlm.nih.gov/BLAST/).…”

A noteworthy record of endophytic Quambalaria cyanescens from Punica granatum in Iran.

“…Quambalaria cyanescens has been isolated from a broad range of ecological niches, including air, plant materials, soil and insects from several countries in North Africa, North America, South Asia, Australia, Europe and the Middle East (de Hoog & de Vries 1973, Kolařík et al 2006, Abdel-Sater et al 2016 pova et al 2014). This fungal species, as a potential opportunistic pathogen in immunocompromised or debilitated patients, has also been isolated from blood, human skin and hospital-acquired infections in individuals with pneumonia, peritoneal inflammation and invasive pulmonary infection (Jackson et al 1990, Tambini et al 1996, Fan et al 2014, Kuan et al 2015.…”