Diversity of Escherichia coli strains involved in vertebral osteomyelitis and arthritis in broilers in Brazil

Braga, Juliana Fortes Vilarinho; Chanteloup, Nathalie; Trotereau, Angélina; Baucheron, Sylvie; Guabiraba, Rodrigo; Ecco, Roselene; Schouler, Catherine

doi:10.1186/s12917-016-0762-0

Cited by 46 publications

(43 citation statements)

References 43 publications

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“…hly A was reported only in 0.8% of APEC although it was observed in 31% of UPEC [31]. While in Brazil, studies have compared several APEC and ExPEC isolates and did not report cnf 1 and hly A in any avian strains [10, 33]. …”

Section: Discussionmentioning

confidence: 99%

“…Here, we report that 4% of the tested isolates were positive for the ibe A gene. A number of studies compared the prevalence of NMEC-related genes among APEC strains and UPEC observing overlaps between the pathotypes [10, 30, 33]. Ewers et al (2007), described ibe A in 26.2% of APEC which suggested that APEC could be a source for other ExPEC of plasmid and chromosomally located genes [7].…”

Section: Discussionmentioning

confidence: 99%

“…A number of sequence types are shared among poultry and human ExPEC strains, including dominant pandemic clones that have also been reported in cases of colibacillosis in Brazil, [4, 10, 33]. The O6-B2-ST73 clone had not been described in poultry isolates to date, and thus this finding highlights the description of an ST that was up to now considered to be an adapted strain in humans without a recognized food source background [1, 4, 16].…”

Section: Discussionmentioning

confidence: 99%

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Pandemic extra-intestinal pathogenic Escherichia coli (ExPEC) clonal group O6-B2-ST73 as a cause of avian colibacillosis in Brazil

et al. 2017

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Extra-intestinal pathogenic Escherichia coli (ExPEC) represent an emerging pathogen, with pandemic strains increasingly involved in cases of urinary tract infections (UTIs), bacteremia, and meningitis. In addition to affecting humans, the avian pathotype of ExPEC, avian pathogenic E. coli (APEC), causes severe economic losses to the poultry industry. Several studies have revealed overlapping characteristics between APEC and human ExPEC, leading to the hypothesis of a zoonotic potential of poultry strains. However, the description of certain important pandemic clones, such as Sequence Type 73 (ST73), has not been reported in food sources. We characterized 27 temporally matched APEC strains from diverse poultry farms in Brazil belonging to the O6 serogroup because this serogroup is frequently described as a causal factor in UTI and septicemia in humans in Brazil and worldwide. The isolates were genotypically characterized by identifying ExPEC virulence factors, phylogenetically tested by phylogrouping and multilocus sequence type (MLST) analysis, and compared to determine their similarity employing the pulsed field gel electrophoresis (PFGE) technique. The strains harbored a large number of virulence determinants that are commonly described in uropathogenic E. coli (UPEC) and sepsis associated E. coli (SEPEC) strains and, to a lesser extent in neonatal meningitis associated E. coli (NMEC), such as pap (85%), sfa (100%), usp (100%), cnf1 (22%), kpsMTII (66%), hlyA (52%), and ibeA (4%). These isolates also yielded a low prevalence of some genes that are frequently described in APEC, such as iss (37%), tsh, ompT, and hlyF (8% each), and cvi/cva (0%). All strains were classified as part of the B2 phylogroup and sequence type 73 (ST73), with a cluster of 25 strains showing a clonal profile by PFGE. These results further suggest the zoonotic potential of some APEC clonal lineages and their possible role in the epidemiology of human ExPEC, in addition to providing the first description of the O6-B2-ST73 clonal group in poultry.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Pandemic extra-intestinal pathogenic Escherichia coli (ExPEC) clonal group O6-B2-ST73 as a cause of avian colibacillosis in Brazil

et al. 2017

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“…Interestingly, the EC121 strain belongs to ST101, which has been previously reported to be involved in nosocomial outbreaks caused by Metallo-β-lactamases-producing strains in many countries from Europe, Asia, and Oceania [26–30]. Furthermore, ST101 has also been detected among strains of non-outbreak related extraintestinal infections [31–38], water [39], poultry infection [40], retail food [31,41– 43], and healthy human and animal intestinal microbiota [38,44–47], mostly presenting an MDR phenotype. Shrestha et al [48] drew attention to ST101 due to the PDR phenotype presented by some strains of this ST, and mainly because it is not considered a pandemic clone, although it has been isolated worldwide.…”

Section: Discussionmentioning

confidence: 99%

Virulence potential of a multidrug-resistant Escherichia coli strain belonging to the emerging clonal group ST101-B1 isolated from bloodstream infection

Santos

Silva

Valiatti

et al. 2019

Preprint

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28Escherichia coli EC121 is a multidrug-resistant (MDR) strain isolated from bloodstream 29 infection of an inpatient with persistent gastroenteritis and Zone T lymphoma that died 30 due to septic shock. Despite causing an extraintestinal infection, strain EC121 harbors 31 very few known virulence factors associated with extraintestinal pathogenic E. coli 32 (ExPEC). Furthermore, this strain was assigned to phylogenetic group B1, which is 33 usually related to commensals or diarrheagenic E. coli (DEC) strains, being rarely linked 34 to extraintestinal infections. The question rises if E. coli EC121 is opportunistic or does 35 have a true virulence potential. To address this question the genome of strain EC121 was 36 sequenced, and an in vitro characterization of some pathogenic-associated properties was 37 performed. The data retrieved from genome analyses showed that E. coli strain EC121 38 belongs to the O154:H25 serotype, and to the sequence type 101-B1 (ST101-B1), which 39 has been epidemiologically linked to extraintestinal infections and antimicrobial 40 resistance spread as well. Moreover, it is shown to be closely related to Shiga-toxin 41 producing E. coli (STEC). Besides, strain EC121 is an MDR strain harboring 14 42 antimicrobial resistance genes, including bla CTX-M-2 , and more than 50 complete virulence 43 genetic clusters, which are reported to be associated either with DEC or ExPEC, or both. 44Strain EC121 also displays the capacity to adhere to and invade HeLa cells, intestinal 45 Caco-2 cells and bladder T24 cells, as well as the ability to form biofilms on abiotic 46 surfaces, and survive the bactericidal serum complement activity. Altogether, the genetic 47 and phenotypic traits presented by E. coli EC121 may be unveiling a pathogen powered 48 by its multi-drug resistance characteristic. The recognition of such MDR pathogens 49 makes it essential to carry out studies providing accurate information about their virulence 50 potential. Such studies will help in the development of alternative therapies of infection 51 management and spread control of MDR strains. [Research article] [Santos el al., 2019] 3 52 53 Author summary 57 Extraintestinal pathogenic Escherichia coli are mostly associated with phylogenetic 58 group B2 and the majority of the studies regarding extraintestinal infection focus on the 59 most virulent strains, which might also possess multidrug-resistant phenotype, like the 60 ST131 strains. On the other hand, strains that belong to phylogroup B1 and are isolated 61 from extraintestinal infections are almost neglected, being considered as merely 62opportunist pathogens, and the majority of studies reporting strains from this phylogroup 63 focus on antimicrobial resistance. We concentrated our research on one multidrug-64 resistant strain isolated from bloodstream infection that belongs to phylogenetic group B1 65 to enlarge the knowledge about the virulence of this kind of strain. We demonstrated that 66 strain EC121 has full potential to be considered a pathogenic strain, bec...

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“…The presence of ehxA, sat and cnf1 have also been reported in APEC strains (Braga et al, 2016;Cunha et al, 2017). However, their role in pathogenesis is not fully elucidated.…”

Section: Toxinsmentioning

confidence: 97%

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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Diversity of Escherichia coli strains involved in vertebral osteomyelitis and arthritis in broilers in Brazil

Cited by 46 publications

References 43 publications

Pandemic extra-intestinal pathogenic Escherichia coli (ExPEC) clonal group O6-B2-ST73 as a cause of avian colibacillosis in Brazil

Pandemic extra-intestinal pathogenic Escherichia coli (ExPEC) clonal group O6-B2-ST73 as a cause of avian colibacillosis in Brazil

Virulence potential of a multidrug-resistant Escherichia coli strain belonging to the emerging clonal group ST101-B1 isolated from bloodstream infection

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

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